CAZypedia - User contributions [en-ca]

User:Gurvan Michel

2010-08-12T07:19:12Z

Gurvan Michel:

[[Image:Gurvan_Michel-pt.jpg|right| ]]
This is the user page of '''Gurvan Michel'''. I am a permanent researcher of the french national research center "[http://www.cnrs.fr/index.php CNRS]"
(Centre National de Recherche Scientifique), and I am working at the
Marine Station "[http://www.sb-roscoff.fr Station Biologique de Roscoff]", France.

I studied biology at the Institut National Agronomique Paris-Grignon and received my Engineer diploma in 1997. As PhD student I learnt crystallography with Otto Dideberg at the Structural Biology Institute (IBS) in Grenoble and obtained my doctorate in 2000. From 2001-2002 I was research associate in Miroslaw Cygler's group at the Biotechnology Research Institute in Montreal, Canada. I then joined the group of Bernard Kloareg at the Station Biologique de Roscoff, as a permanent researcher of the CNRS. With ^^^Mirjam Czjzek^^^ I participated in the development of a crystallography group in Roscoff. I am now member of the group "[http://www.sb-roscoff.fr/en/glycobiologie.html marine glycobiology]" where my research strategy is to combine '''genomics approaches''' with '''structural methods''' to investigate the function of '''novel polysaccharidases''' from marine bacteria and algae. In collaboration with Rudolph Amann's group (MPI Bremen, Germany) and the Genoscope (Evry, France), I have coordinated together with Tristan Barbeyron the genome project of the Flavobacterium ''Zobellia galactanivorans'', a key marine polysaccharide degrader. I also analysed the carbohydrate metabolism in the genome of the model brown alga, ''Ectocarpus siliculosus''.

[[Category:Contributors|Michel, Gurvan]]

Glycoside Hydrolase Family 82

2010-02-16T17:05:27Z

Gurvan Michel:

{{UnderConstruction}}
* [[Author]]s: ^^^Mirjam Czjzek^^^ and ^^^Gurvan Michel^^^
* [[Responsible Curator]]: ^^^Mirjam Czjzek^^^
----

<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GH82'''
|-
|'''Clan'''
|none
|-
|'''Mechanism'''
|inverting
|-
|'''Active site residues'''
|not known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |http://www.cazy.org/fam/GH82.html
|}
</div>

== Substrate specificities ==
The two known members of [[glycoside hydrolase]] family 82 enzymes cleave the β-1,4 galactosidic bond of the marine algal polysaccharide iota-carrageenan <cite>1</cite> yielding products of the neocarrabiose series.

== Kinetics and Mechanism ==
Family 82 enzymes are [[inverting]] enzymes, as first shown by NMR <cite>1</cite> on the iota-carrageenase from ''Alteromonas fortis''.

== Catalytic Residues ==
From structural analysis predicted to be two out of the three candidate amino acids Glu245, Asp247 or Glu310 in the ''A. fortis'' iota-carrageenase <cite>2</cite>.

== Three-dimensional structures ==
To date, a crystal structure has only been determined for the iota-carrageenase from ''A. fortis'' <cite>2</cite>. The crystal structure of a product complex has shed light on the existance of domain movement of domain A that is closed around the oligo-carrageenan in the complexed form and open in the uncomplexed enzyme <cite>3</cite>.

== Family Firsts ==
;First sequence identification and family creation: iota-carrageenase sequences have been first reported for enzymes from ''A. fortis'' and ''Z. galactanivorans'' <cite>1</cite>.
;First sterochemistry determination: GH82 enzymes are inverting as shown by NMR <cite>1</cite>.
;First general acid residue identification: not determined yet.
;First general base residue identification: not determined yet.
;First 3-D structure:
iota-carrageenase from ''A. fortis'' <cite>2</cite>. The structure belongs to the β-helix fold ([http://www.rcsb.org/pdb/explore/explore.do?structureId=1H80 PDB 1h80] and [http://www.rcsb.org/pdb/explore/explore.do?structureId=1KTW PDB 1ktw]).

== References ==
<biblio>
#1 pmid=10934194
#2 pmid=11493601
#3 pmid=14623184

</biblio>

[[Category:Glycoside Hydrolase Families|GH082]]

Glycoside Hydrolase Family 82

2010-02-16T17:04:48Z

Gurvan Michel:

{{UnderConstruction}}
* [[Author]]s: ^^^Gurvan Michel^^^ and ^^^Mirjam Czjzek^^^
* [[Responsible Curator]]: ^^^Mirjam Czjzek^^^
----

<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GH82'''
|-
|'''Clan'''
|none
|-
|'''Mechanism'''
|inverting
|-
|'''Active site residues'''
|not known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |http://www.cazy.org/fam/GH82.html
|}
</div>

== Substrate specificities ==
The two known members of [[glycoside hydrolase]] family 82 enzymes cleave the β-1,4 galactosidic bond of the marine algal polysaccharide iota-carrageenan <cite>1</cite> yielding products of the neocarrabiose series.

== Kinetics and Mechanism ==
Family 82 enzymes are [[inverting]] enzymes, as first shown by NMR <cite>1</cite> on the iota-carrageenase from ''Alteromonas fortis''.

== Catalytic Residues ==
From structural analysis predicted to be two out of the three candidate amino acids Glu245, Asp247 or Glu310 in the ''A. fortis'' iota-carrageenase <cite>2</cite>.

== Three-dimensional structures ==
To date, a crystal structure has only been determined for the iota-carrageenase from ''A. fortis'' <cite>2</cite>. The crystal structure of a product complex has shed light on the existance of domain movement of domain A that is closed around the oligo-carrageenan in the complexed form and open in the uncomplexed enzyme <cite>3</cite>.

== Family Firsts ==
;First sequence identification and family creation: iota-carrageenase sequences have been first reported for enzymes from ''A. fortis'' and ''Z. galactanivorans'' <cite>1</cite>.
;First sterochemistry determination: GH82 enzymes are inverting as shown by NMR <cite>1</cite>.
;First general acid residue identification: not determined yet.
;First general base residue identification: not determined yet.
;First 3-D structure:
iota-carrageenase from ''A. fortis'' <cite>2</cite>. The structure belongs to the β-helix fold ([http://www.rcsb.org/pdb/explore/explore.do?structureId=1H80 PDB 1h80] and [http://www.rcsb.org/pdb/explore/explore.do?structureId=1KTW PDB 1ktw]).

== References ==
<biblio>
#1 pmid=10934194
#2 pmid=11493601
#3 pmid=14623184

</biblio>

[[Category:Glycoside Hydrolase Families|GH082]]

User:Gurvan Michel

2010-02-16T17:04:06Z

Gurvan Michel:

[[Image:Gurvan_Michel-pt.jpg|right| ]]
This is the user page of '''Gurvan Michel'''. I am a permanent researcher of the french national research center "[http://www.cnrs.fr/index.php CNRS]"
(Centre National de Recherche Scientifique), and I am working at the
Marine Station "[http://www.sb-roscoff.fr Station Biologique de Roscoff]", France.

I studied biology at the Institut National Agronomique Paris-Grignon and received my Engineer diploma in 1997. As PhD student I learnt crystallography with Otto Dideberg at the Structural Biology Institute (IBS) in Grenoble and obtained my doctorate in 2000. From 2001-2002 I was research associate in Miroslaw Cygler's group at the Biotechnology Research Institute in Montreal, Canada. I then joined the group of Bernard Kloareg at the Station Biologique de Roscoff, as a permanent researcher of the CNRS. With Mirjam Czjzek I participated to the development of a crystallography group in Roscoff. I am now member of the group "[http://www.sb-roscoff.fr/UMR7139/en/interaction.html marine glycobiology]" where my research strategy is to combine '''genomics approaches''' with '''structural methods''' to investigate the function of '''novel polysaccharidases''' from marine bacteria and algae. In collab oration with Rudolph Amann's group (MPI Bremen, Germany) and the Genoscope (Evry, France)I have coordinated together with Tristan Barbeyron the genome project of the Flavobacterium ''Zobellia galactanivorans'', a key marine polysaccharide degrader. I also analysed the carbohydrate metabolism in the genome of the model brown alga, ''Ectocarpus siliculosus''.

[[Category:Contributors|Michel, Gurvan]]

User:Gurvan Michel

2010-02-16T17:03:16Z

Gurvan Michel:

[[Image:Gurvan_Michel-pt.jpg|right| ]]
This is the user page of '''Gurvan Michel'''. I am a permanent researcher of the french national research center "[http://www.cnrs.fr/index.php CNRS]"
(Centre National de Recherche Scientifique), and I am working at the
Marine Station "[http://www.sb-roscoff.fr Station Biologique de Roscoff]", France.

I studied biology at the Institut National Agronomique Paris-Grignon and received my Engineer diploma in 1997. As PhD student I learnt crystallography with Otto Dideberg at the Structural Biology Institute (IBS) in Grenoble and obtained my doctorate in 2000. From 2001-2002 I was research associate in Miroslaw Ciegler's group at the Biotechnology Research Institute in Montreal, Canada. I then joined the group of Bernard Kloareg at the Station Biologique de Roscoff, as a permanent researcher of the CNRS. With Mirjam Czjzek I participated to the development of a crystallography group in Roscoff. I am now member of the group "[http://www.sb-roscoff.fr/UMR7139/en/interaction.html marine glycobiology]" where my research strategy is to combine '''genomics approaches''' with '''structural methods''' to investigate the function of '''novel polysaccharidases''' from marine bacteria and algae. In collab oration with Rudolph Amann's group (MPI Bremen, Germany) and the Genoscope (Evry, France)I have coordinated together with Tristan Barbeyron the genome project of the Flavobacterium ''Zobellia galactanivorans'', a key marine polysaccharide degrader. I also analysed the carbohydrate metabolism in the genome of the model brown alga, ''Ectocarpus siliculosus''.

[[Category:Contributors|Michel, Gurvan]]

User:Gurvan Michel

2010-02-16T17:02:19Z

Gurvan Michel: Created page with ' This is the user page of '''Gurvan Michel'''. I am a permanent researcher of the french national research center "[http://www.cnrs.fr/index…'

[[Image:Gurvan_Michel-pt.jpg|right| ]]
This is the user page of '''Gurvan Michel'''. I am a permanent researcher of the french national research center "[http://www.cnrs.fr/index.php CNRS]"
(Centre National de Recherche Scientifique), and I am working at the
Marine Station "[http://www.sb-roscoff.fr Station Biologique de Roscoff]", France.

Normal 0 21 MicrosoftInternetExplorer4 I studied biology at the Institut National Agronomique Paris-Grignon and received my Engineer diploma in 1997. As PhD student I learnt crystallography with Otto Dideberg at the Structural Biology Institute (IBS) in Grenoble and obtained my doctorate in 2000. From 2001-2002 I was research associate in Miroslaw Ciegler's group at the Biotechnology Research Institute in Montreal, Canada. I then joined the group of Bernard Kloareg at the Station Biologique de Roscoff, as a permanent researcher of the CNRS. With Mirjam Czjzek I participated to the development of a crystallography group in Roscoff. I am now member of the group "[http://www.sb-roscoff.fr/UMR7139/en/interaction.html marine glycobiology]" where my research strategy is to combine '''genomics approaches''' with '''structural methods''' to investigate the function of '''novel polysaccharidases''' from marine bacteria and algae. In collab oration with Rudolph Amann's group (MPI Bremen, Germany) and the Genoscope (Evry, France)I have coordinated together with Tristan Barbeyron the genome project of the Flavobacterium ''Zobellia galactanivorans'', a key marine polysaccharide degrader. I also analysed the carbohydrate metabolism in the genome of the model brown alga, ''Ectocarpus siliculosus''.

[[Category:Authors|Michel, Gurvan]]

File:Gurvan Michel-pt.jpg

2010-02-16T16:45:09Z

Gurvan Michel: