CAZypedia - User contributions [en-ca]

User:Jonathon Briggs

2018-04-12T23:56:50Z

Jonathon Briggs:

[[File:Picture-1291.jpg|200px|right]]

Jonathon Briggs completed his undergraduate degree in Medical Microbiology and Immunology at Newcastle University before undertaking his PhD studies in the lab of Professor ^^^Harry Gilbert^^^. His PhD project focused on utilization of various glycan substrates encountered by the prominent gut microbes ''Bacteroides thetaiotaomicron'' and ''Bacteroides ovatus''.

Jonathon contributed to the characterization of the ''B. ovatus'' xylan utilization system <cite>Rogowski2015</cite>, the ''B. thetaiotaomicron'' galactan, arabinan, rhamnogalacturonan I <cite>Ndeh2017</cite> and II utilization systems <cite>luis2018</cite>. In the process of characterizing the arabinan and galactan utilization systems he identified the founding members of Glycoside Hydrolase families [[GH146]] and [[GH147]] <cite>luis2018</cite>.

Jonathon has recently joined the lab of ^^^Brumer^^^ at the University of British Columbia.

----

<biblio>
#Rogowski2015 pmid=26112186
#Ndeh2017 pmid=28329766
#Jones2017 pmid=28588026
#luis2018 pmid=29255254

</biblio>


[[Category:Contributors|Briggs,Jonathon]]

File:Picture-1291.jpg

2018-04-12T23:54:16Z

Jonathon Briggs: User Profile Image Jon Briggs

User Profile Image Jon Briggs

User:Jonathon Briggs

2018-04-12T22:10:26Z

Jonathon Briggs:

Jonathon Briggs completed his undergraduate degree in Medical Microbiology and Immunology at Newcastle University before undertaking his PhD studies in the lab of Professor Harry Gilbert. His PhD project focused on utilization of various glycan substrates encountered by the prominent gut microbes ''Bacteroides thetaiotaomicron'' and ''Bacteroides ovatus''.

Jonathon contributed to the characterization of the ''B. ovatus'' xylan utilization system <cite>Rogowski2015</cite>, the ''B. thetaiotaomicron'' galactan, arabinan, rhamnogalacturonan I <cite>Ndeh2017</cite> and II utilization systems <cite>luis2018</cite>. In the process of characterizing the arabinan and galactan utilization systems he identified the founding members of Glycoside Hydrolase families 146 and 147 <cite>luis2018</cite>.

Jonathon has recently joined the Brumer lab at the University of British Columbia.

----

<biblio>
#Rogowski2015 pmid=26112186
#Ndeh2017 pmid=28329766
#Jones2017 pmid=28588026
#luis2018 pmid=29255254

</biblio>


[[Category:Contributors|Briggs,Jonathon]]

User:Jonathon Briggs

2018-04-12T22:08:21Z

Jonathon Briggs:

Jonathon Briggs completed his undergraduate degree in Medical Microbiology and Immunology at Newcastle University before undertaking his PhD studies in the lab of Professor Harry Gilbert. His PhD project focused on utilization of various glycan substrates encountered by the prominent gut microbes ''Bacteroides thetaiotaomicron'' and ''Bacteroides ovatus''.

Jonathon contributed to the characterization of the ''B. ovatus'' xylan utilization system <cite>Rogowski2015</cite>, the ''B. thetaiotaomicron'' galactan, arabinan, rhamnogalacturonan I<cite>Ndeh2017</cite> and II utilization systems <cite>luis2018</cite>. In the process of characterizing the arabinan and galactan utilization systems he identified the founding members of Glycoside Hydrolase families 146 and 147 <cite>luis2018</cite>.

Jonathon has recently joined the Brumer lab at the University of British Columbia.

----

<biblio>
#Rogowski2015 pmid=26112186
#Ndeh2017 pmid=28329766
#Jones2017 pmid=28588026
#luis2018 pmid=29255254

</biblio>


[[Category:Contributors|Briggs,Jonathon]]

Glycoside Hydrolase Family 147

2018-01-18T15:10:29Z

Jonathon Briggs:

{{UnderConstruction}}
* [[Author]]: ^^^Jonathon Briggs^^^
* [[Responsible Curator]]: ^^^Harry Gilbert^^^
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GH147'''
|-
|'''Clan'''
|GH-A
|-
|'''Mechanism'''
|retaining
|-
|'''Active site residues'''
|known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |{{CAZyDBlink}}GH147.html
|}
</div>


== Substrate specificities ==
The founding member of GH147, BACOVA_05493 a β1,4-galactosidase demonstrates a preference towards longer oligosaccharides releasing galacopyranose from the oligosaccharide chain. BACOVA_ is unable to hydrolyse galactobiose <cite>Luis2017</cite>.

== Kinetics and Mechanism ==
NMR analysis of the hydrolysis product revealed a retaining mechanism of action <cite>Luis2017</cite>.

== Catalytic Residues ==
The catalytic nucleophile and general acid/base residues of the founding member of GH147, BACOVA_05493, were identified as Glu300 and Glu203, respectively <cite>Luis2017</cite>.

== Three-dimensional structures ==
Currently there is no crystal structure of any member of GH147.

== Family Firsts ==
;First stereochemistry determination: BACOVA_05493 from B. ovatus <cite>Luis2017</cite>.
;First catalytic nucleophile identification: BACOVA_05493 from B. ovatus <cite>Luis2017</cite>.
;First general acid/base residue identification: BACOVA_05493 from B. ovatus <cite>Luis2017</cite>.
;First 3-D structure: Currently not available.

== References ==
<biblio>
#Luis2017 pmid=29255254
</biblio>

[[Category:Glycoside Hydrolase Families|GH147]]

Glycoside Hydrolase Family 146

2018-01-18T15:02:08Z

Jonathon Briggs:

{{UnderConstruction}}
* [[Author]]: ^^^Jonathon Briggs^^^
* [[Responsible Curator]]: ^^^Harry Gilbert^^^
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GH146'''
|-
|'''Clan'''
|GH-A
|-
|'''Mechanism'''
|retaining
|-
|'''Active site residues'''
|known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |{{CAZyDBlink}}GH146.html
|}
</div>


== Substrate specificities ==
This glycoside hydrolase family has only one assigned activity to date, β-arabinofuranosidease. The founding member of this family, BT_0349, was shown to cleave both β1,2- and β1,3-linked arabinofuranose sidechains present in branched sugar beet arabinan <cite>Luis2017</cite>.

== Kinetics and Mechanism ==
The only characterised GH146 enzyme, BT_0349 displays exo-activity on β-linked arabinofuranose by deploying a retaining mechanism, based on identification of catalytic residues <cite>Luis2017</cite>.

== Catalytic Residues ==
The catalytic nucleophile and general acid/base residues of the founding member of GH146, BT_0349, were identified as Cys414 and Glu318, respectively <cite>Luis2017</cite>.

== Three-dimensional structures ==
The crystal structure of BT_0349, solved using SAD methods to a resolution of 2.1 A, revealed a four-domain structure. The N-terminal catalytic domain comprises an (α/ α)5 barrel followed by three β-sandwich domains (1, 2 & 3). An arabinofuranose is present in the active site pocket of the catalytic domain, while a zinc atom is coordinated by three cystines and a glutamate in the same domain. The catalytic apparatus is completely conserved with GH127, however GH127 lacks β-sandwich domain 3 which is positioned over the active site, effectively burying the bound arabinofuranose <cite>Luis2017</cite>.

== Family Firsts ==
;First stereochemistry determination: BT_0349 from ''B. thetaiotaomicron'' <cite>Luis2017</cite>.
;First catalytic nucleophile identification: BT_0349 from ''B. thetaiotaomicron'' <cite>Luis2017</cite>.
;First general acid/base residue identification: BT_0349 from ''B. thetaiotaomicron'' <cite>Luis2017</cite>.
;First 3-D structure: BT_0349 from ''B. thetaiotaomicron'' <cite>Luis2017</cite>.

== References ==
<biblio>
#Luis2017 pmid=29255254
</biblio>

[[Category:Glycoside Hydrolase Families|GH146]]