{{UnderConstruction}}
* [[Author]]: ^^^Kiyotaka Fujita^^^
* [[Responsible Curator]]: ^^^Shinya Fushinobu^^^
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GH121'''
|-
|'''Clan'''
|GH-x
|-
|'''Mechanism'''
|retaining
|-
|'''Active site residues'''
|not known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |{{CAZyDBlink}}GH121.html
|}
</div>


== Substrate specificities ==
This family of [[glycoside hydrolases]] contains β-L-arabinobiosidase, which was recently established for HypBA2 from ''Bifidobacterium longum'' JCM 1217<cite>Fujita2011A</cite>.HypBA2 liberated Ara''f''β1-2Ara''f''(β-Ara2) from unmodified Ara''f''β1-2Ara''f''β1-2Ara''f''β-Hydroxyproline (Ara3-Hyp) but not Ara''f''α1-3Ara''f''β1-2Ara''f''β1-2Ara''f''β-Hyp(Ara4-Hyp) or Arafβ1-2Arafβ-Hyp (Ara2-Hyp).

== Kinetics and Mechanism ==
HypBA2 is a retaining enzyme.The stereochemical course of the reaction was shown by transglycosylation activity toward 1-alkanols, and following 1H-NMR and 13C-NMR analysis of the methanol-adducted transglycosylation product.

== Catalytic Residues ==
Not known.

== Three-dimensional structures ==
Not known.

== Family Firsts ==
;First stereochemistry determination: This was determined with HypBA2 enzyme by measurement of glycosyl transfer reactions to methanol and the 1H-NMR and 13C-NMR spectra.
;First catalytic nucleophile identification: No experimental proof.
;First general acid/base residue identification: No experimental proof.
;First 3-D structure: Not known.

== References ==
<biblio>
#Fujita2011A pmid=21149454
</biblio>

[[Category:Glycoside Hydrolase Families|GH121]]

2012-11-04T23:58:15Z

Kiyotaka Fujita:

Glycoside Hydrolase Family 127

2012-04-27T09:38:36Z

Kiyotaka Fujita:

{{UnderConstruction}}
* [[Author]]: ^^^Kiyotaka Fujita^^^
* [[Responsible Curator]]: ^^^Shinya Fushinobu^^^
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GH127'''
|-
|'''Clan'''
|GH-x
|-
|'''Mechanism'''
|retaining
|-
|'''Active site residues'''
|not known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |{{CAZyDBlink}}GH127.html
|}
</div>


== Substrate specificities ==
This family of [[glycoside hydrolases]] contains β-L-arabinofuranosidase, which was recently established for HypBA1 from ''Bifidobacterium longum'' JCM 1217<cite>Fujita2011B</cite>. The enzymes belonging to this family are also members of Pfam DUF1680 family, conserved in many species of bacteria, actinomycetes, fugi, and plants.

== Kinetics and Mechanism ==
Content is to be added here.

== Catalytic Residues ==
Content is to be added here.

== Three-dimensional structures ==
Content is to be added here.

== Family Firsts ==
;First stereochemistry determination: This was determined with HypBA1 enzyme by measurement of glycosyl transfer reactions to methanol and the 1H-NMR and13C-NMR spectra.
;First catalytic nucleophile identification: Content is to be added here.
;First general acid/base residue identification: Content is to be added here.
;First 3-D structure: Content is to be added here.

== References ==
<biblio>
#Fujita2011B Fujita K, Takashi Y, Obuchi E, Kitahara K, Suganuma T.(2011)Characterization of a novel β-L-Arabinofuranosidase in Bifidobacterium longum: functional elucidation of A DUF1680 family member. J Biol Chem. 286(44), 38079-85.[http://www.ncbi.nlm.nih.gov/pubmed/21914802]
</biblio>

[[Category:Glycoside Hydrolase Families|GH127]]

Glycoside Hydrolase Family 127

2012-04-27T09:25:59Z

Kiyotaka Fujita:

{{UnderConstruction}}
* [[Author]]: ^^^Kiyotaka Fujita^^^
* [[Responsible Curator]]: ^^^Shinya Fushinobu^^^
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GH127'''
|-
|'''Clan'''
|GH-x
|-
|'''Mechanism'''
|retaining
|-
|'''Active site residues'''
|not known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |{{CAZyDBlink}}GH127.html
|}
</div>


== Substrate specificities ==
This family of [[glycoside hydrolases]] contains β-L-arabinofuranosidase, which was recently established for HypBA1 from ''Bifidobacterium longum'' JCM 1217<cite>Fujita2011B</cite>.

== Kinetics and Mechanism ==
Content is to be added here.

== Catalytic Residues ==
Content is to be added here.

== Three-dimensional structures ==
Content is to be added here.

== Family Firsts ==
;First stereochemistry determination: This was determined with HypBA1 enzyme by measurement of glycosyl transfer reactions to methanol and the 1H-NMR and13C-NMR spectra.
;First catalytic nucleophile identification: Content is to be added here.
;First general acid/base residue identification: Content is to be added here.
;First 3-D structure: Content is to be added here.

== References ==
<biblio>
#Fujita2011B Fujita K, Takashi Y, Obuchi E, Kitahara K, Suganuma T.(2011)Characterization of a novel β-L-Arabinofuranosidase in Bifidobacterium longum: functional elucidation of A DUF1680 family member. J Biol Chem. 286(44), 38079-85.[http://www.ncbi.nlm.nih.gov/pubmed/21914802]
</biblio>

[[Category:Glycoside Hydrolase Families|GH127]]

Glycoside Hydrolase Family 121

2012-04-27T09:24:50Z

Kiyotaka Fujita:

Glycoside Hydrolase Family 121

2012-04-27T09:13:31Z

Kiyotaka Fujita:

Glycoside Hydrolase Family 127

2012-04-27T05:31:12Z

2012-03-22T12:31:37Z

Kiyotaka Fujita:

User:Kiyotaka Fujita

2012-03-22T12:29:12Z

Kiyotaka Fujita:

User:Kiyotaka Fujita

2012-03-22T12:22:37Z

Kiyotaka Fujita:

User:Kiyotaka Fujita

2012-03-21T12:20:18Z

Kiyotaka Fujita:

User:Kiyotaka Fujita

2012-03-21T12:17:49Z

Kiyotaka Fujita:

User:Kiyotaka Fujita

2012-03-21T12:11:37Z

Kiyotaka Fujita: