CAZypedia - User contributions [en-ca]

Glycoside Hydrolase Family 12

2010-04-05T22:42:00Z

Mats Sandgren:

{{UnderConstruction}}
* [[Author]]: ^^^Mats Sandgren^^^
* [[Responsible Curator]]: ^^^Mats Sandgren^^^
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GHnn'''
|-
|'''Clan'''
|GH-C
|-
|'''Mechanism'''
|retaining
|-
|'''Active site residues'''
|known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |http://www.cazy.org/fam/GH12.html
|}
</div>


== Substrate specificities ==
The substrate specificities found among the [[glycoside hydrolases]] of family 12 are: ''[[endo]]''-β-1,4-glucanase (EC 3.2.1.4), ''xyloglucan hydrolase (EC 3.2.1.151), ''[[endo]]''-β-1,3-1,4-glucanase (EC 3.2.1.73), and xyloglucan ''[[endo]]''-transglycosylase (EC 2.4.1.207),.

== Kinetics and Mechanism ==
GH Family 12 enzymes are [[retaining]] enzymes, as first shown by NMR studdies <cite>1</cite> on endoglucanase 3 from ''Humicola insolens'', and is believed to follow a classical [[Koshland double-displacement mechanism]] in which a glycosyl-enzyme intermediate is formed and subsequently this intermediate is hydrolysed via oxocarbenium-ion transition states. No detailed studies involving both steady state and pre-steady state kinetic have yet been reported for GH family 12.

== Catalytic Residues ==
The [[catalytic nucleophile]] and the [[general acid/base]] catalyst of GH family 12 enzymes was initialy predicted by sequence homology to the xylanase members of GH family 11, a glycoside hydrolase family where the [[catalytic nucleophile]] was first identified in the ''Bacillus circulans'' [[endo]]-xylanase through trapping of the 2-deoxy-2-fluoroxylobiosyl-enzyme [[intermediate]] and subsequent peptide mapping via LC-MS/MS technologies <cite>2</cite>. GH family 11 and 12 together form clan C of related GH families. The prediction of the [[catalytic nucleophile]] and the [[general acid/base]] of GH family 12 enzymes was later confirmed to be right whe the first three dimensional structure of a GH family 12 enzyme was determined, that of ''Streptomyces Lividans'' CelB <cite>3</cite>. The [[catalytic nucleophile]] was subsequentialy confirmed in ''Streptomyces Lividans'' CelB to be Glu 120 by using the same labeling strategy used for detecting the [[catalytic nucleophile]] of GH family 11 <cite>4</cite>.

== Three-dimensional structures ==
Content is to be added here.

== Family Firsts ==
;First sterochemistry determination: ''Humicola insolens'' endoglucanase 3 by NMR <cite>1</cite>.
;First catalytic nucleophile identification: .
;First general acid/base residue identification: .
;First 3-D structure: .

== References ==
<biblio>
#1 pmid=8223652
#2 pmid=7911679
#2 pmid=9440876
#4 pmid=9806895

</biblio>

[[Category:Glycoside Hydrolase Families|GH012]]

Glycoside Hydrolase Family 12

2010-04-05T22:15:57Z

Mats Sandgren:

{{UnderConstruction}}
* [[Author]]: ^^^Mats Sandgren^^^
* [[Responsible Curator]]: ^^^Mats Sandgren^^^
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GHnn'''
|-
|'''Clan'''
|GH-C
|-
|'''Mechanism'''
|retaining
|-
|'''Active site residues'''
|known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |http://www.cazy.org/fam/GH12.html
|}
</div>


== Substrate specificities ==
The substrate specificities found among the [[glycoside hydrolases]] of family 12 are: ''[[endo]]''-β-1,4-glucanase (EC 3.2.1.4), ''xyloglucan hydrolase (EC 3.2.1.151), ''[[endo]]''-β-1,3-1,4-glucanase (EC 3.2.1.73), and xyloglucan ''[[endo]]''-transglycosylase (EC 2.4.1.207),.

== Kinetics and Mechanism ==
GH Family 12 enzymes are [[retaining]] enzymes, as first shown by NMR studdies <cite>1</cite> on endoglucanase 3 from ''Humicola insolens'', and follow a classical [[Koshland double-displacement mechanism]]. No detailed studies involving both steady state and pre-steady state kinetic have yet been reported for this family.

== Catalytic Residues ==
The [[catalytic nucleophile]] of GH family 12 enzymes was initialy predicted by sequence homology to the xylanase members of GH family 11, a glycoside hydrolase family where the [[catalytic nucleophile]] was first identified in the ''Bacillus circulans'' [[endo]]-xylanase through trapping of the 2-deoxy-2-fluoroxylobiosyl-enzyme [[intermediate]] and subsequent peptide mapping via LC-MS/MS technologies <cite>2</cite>. GH family 11 and 12 together form clan C of related GH families. The [[catalytic nucleophile]] was subsequentialy confirmed in Streptomyces Lividans CelB to be Glu 120 by using the same labeling strategy used for detecting the [[catalytic nucleophile]] of GH family 11 <cite>3</cite>.

== Three-dimensional structures ==
Content is to be added here.

== Family Firsts ==
;First sterochemistry determination: ''Humicola insolens'' endoglucanase 3 by NMR <cite>1</cite>.
;First catalytic nucleophile identification: .
;First general acid/base residue identification: .
;First 3-D structure: .

== References ==
<biblio>
#1 pmid=8223652
#2 pmid=7911679
#3 pmid=9806895

</biblio>

[[Category:Glycoside Hydrolase Families|GH012]]

Glycoside Hydrolase Family 12

2010-04-05T20:59:53Z

Mats Sandgren:

{{UnderConstruction}}
* [[Author]]: ^^^Mats Sandgren^^^
* [[Responsible Curator]]: ^^^Mats Sandgren^^^
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GHnn'''
|-
|'''Clan'''
|GH-C
|-
|'''Mechanism'''
|retaining
|-
|'''Active site residues'''
|known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |http://www.cazy.org/fam/GH12.html
|}
</div>


== Substrate specificities ==
The substrate specificities found among the [[glycoside hydrolases]] of family 12 are: ''[[endo]]''-β-1,4-glucanase (EC 3.2.1.4), ''xyloglucan hydrolase (EC 3.2.1.151), ''[[endo]]''-β-1,3-1,4-glucanase (EC 3.2.1.73), and xyloglucan ''[[endo]]''-transglycosylase (EC 2.4.1.207),.

== Kinetics and Mechanism ==
GH Family 12 enzymes are [[retaining]] enzymes, as first shown by NMR studdies <cite>1</cite> on endoglucanase 3 from ''Humicola insolens'', and follow a classical [[Koshland double-displacement mechanism]]. No detailed studies involving both steady state and pre-steady state kinetic have yet been reported for this family.

== Catalytic Residues ==
The [[catalytic nucleophile]] of GH family 12 enzymes was initialy predicted by sequence homology to the xylanase members of GH family 11, a glycoside hydrolase family where the [[catalytic nucleophile]] was first identified in the ''Bacillus circulans'' [[endo]]-xylanase through trapping of the 2-deoxy-2-fluoroxylobiosyl-enzyme [[intermediate]] and subsequent peptide mapping via LC-MS/MS technologies <cite>2</cite>. GH family 11 and 12 together form clan C of related GH families. The predicted [[catalytic nucleophile]] was subsequentialy confirmed through

== Three-dimensional structures ==
Content is to be added here.

== Family Firsts ==
;First sterochemistry determination: ''Humicola insolens'' endoglucanase 3 by NMR <cite>1</cite>.
;First catalytic nucleophile identification: .
;First general acid/base residue identification: .
;First 3-D structure: .

== References ==
<biblio>
#1 pmid=8223652
#2 pmid=7911679

</biblio>

[[Category:Glycoside Hydrolase Families|GH012]]

Glycoside Hydrolase Family 12

2010-04-05T20:59:28Z

Mats Sandgren:

{{UnderConstruction}}
* [[Author]]: ^^^Mats Sandgren^^^
* [[Responsible Curator]]: ^^^Mats Sandgren^^^
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GHnn'''
|-
|'''Clan'''
|GH-C
|-
|'''Mechanism'''
|retaining
|-
|'''Active site residues'''
|known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |http://www.cazy.org/fam/GH12.html
|}
</div>


== Substrate specificities ==
The substrate specificities found among the [[glycoside hydrolases]] of family 12 are: ''[[endo]]''-β-1,4-glucanase (EC 3.2.1.4), ''xyloglucan hydrolase (EC 3.2.1.151), ''[[endo]]''-β-1,3-1,4-glucanase (EC 3.2.1.73), and xyloglucan ''[[endo]]''-transglycosylase (EC 2.4.1.207),.

== Kinetics and Mechanism ==
GH Family 12 enzymes are [[retaining]] enzymes, as first shown by NMR studdies <cite>1</cite> on endoglucanase 3 from ''Humicola insolens'', and follow a classical [[Koshland double-displacement mechanism]]. No detailed studies involving both steady state and pre-steady state kinetic have yet been reported for this family.

== Catalytic Residues ==
The [[catalytic nucleophile]] of GH family 12 enzymes was initialy predicted by sequence homology to the xylanase members of GH family 11, a glycoside hydrolase family where the [[catalytic nucleophile]] was first identified in the ''Bacillus circulans'' [[endo]]-xylanase through trapping of the 2-deoxy-2-fluoroxylobiosyl-enzyme [[intermediate]] and subsequent peptide mapping via LC-MS/MS technologies <cite>2</cite>. GH family 11 and 12 together form clan C of related GH families. The predicted [[catalytic nucleophile]] was subsequentialy confirmed through

== Three-dimensional structures ==
Content is to be added here.

== Family Firsts ==
;;First sterochemistry determination: ''Humicola insolens'' endoglucanase 3 by NMR <cite>1</cite>.
;First catalytic nucleophile identification: .
;First general acid/base residue identification: .
;First 3-D structure: .

== References ==
<biblio>
#1 pmid=8223652
#2 pmid=7911679

</biblio>

[[Category:Glycoside Hydrolase Families|GH012]]

Glycoside Hydrolase Family 12

2010-04-05T20:41:11Z

Mats Sandgren:

{{UnderConstruction}}
* [[Author]]: ^^^Mats Sandgren^^^
* [[Responsible Curator]]: ^^^Mats Sandgren^^^
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GHnn'''
|-
|'''Clan'''
|GH-C
|-
|'''Mechanism'''
|retaining
|-
|'''Active site residues'''
|known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |http://www.cazy.org/fam/GH12.html
|}
</div>


== Substrate specificities ==
The substrate specificities found among the [[glycoside hydrolases]] of family 12 are: ''[[endo]]''-β-1,4-glucanase (EC 3.2.1.4), ''xyloglucan hydrolase (EC 3.2.1.151), ''[[endo]]''-β-1,3-1,4-glucanase (EC 3.2.1.73), and xyloglucan ''[[endo]]''-transglycosylase (EC 2.4.1.207),.

== Kinetics and Mechanism ==
GH Family 12 enzymes are [[retaining]] enzymes, as first shown by NMR studdies <cite>1</cite> on endoglucanase 3 from ''Humicola insolens'', and follow a classical [[Koshland double-displacement mechanism]]. No detailed studies involving both steady state and pre-steady state kinetic have yet been reported for this family.

== Catalytic Residues ==
The [[catalytic nucleophile]] of GH family 12 enzymes was initialy predicted to be by sequence homology to the xylanase members of GH family 11, a glycoside hydrolase family where the [[catalytic nucleophile]] was first identified in the ''Bacillus circulans'' [[endo]]-xylanase through trapping of the 2-deoxy-2-fluoroxylobiosyl-enzyme [[intermediate]] and subsequent peptide mapping via LC-MS/MS technologies <cite>2</cite>. GH family 11 and 12 together form GH clan C of related GH families. The predicted [[catalytic nucleophile]] was subsequentialy confirmed through

== Three-dimensional structures ==
Content is to be added here.

== Family Firsts ==
;;First sterochemistry determination: ''Humicola insolens'' endoglucanase 3 by NMR <cite>1</cite>.
;First catalytic nucleophile identification: .
;First general acid/base residue identification: .
;First 3-D structure: .

== References ==
<biblio>
#1 pmid=8223652
#2 pmid=7911679

</biblio>

[[Category:Glycoside Hydrolase Families|GH012]]

Glycoside Hydrolase Family 12

2010-04-05T20:13:01Z

Mats Sandgren:

{{UnderConstruction}}
* [[Author]]: ^^^Mats Sandgren^^^
* [[Responsible Curator]]: ^^^Mats Sandgren^^^
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GHnn'''
|-
|'''Clan'''
|GH-C
|-
|'''Mechanism'''
|retaining
|-
|'''Active site residues'''
|known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |http://www.cazy.org/fam/GH12.html
|}
</div>


== Substrate specificities ==
The substrate specificities found among the [[glycoside hydrolases]] of family 12 are: ''[[endo]]''-β-1,4-glucanase (EC 3.2.1.4), ''xyloglucan hydrolase (EC 3.2.1.151), ''[[endo]]''-β-1,3-1,4-glucanase (EC 3.2.1.73), and xyloglucan ''[[endo]]''-transglycosylase (EC 2.4.1.207),.

== Kinetics and Mechanism ==
GH Family 12 enzymes are [[retaining]] enzymes, as first shown by NMR studdies <cite>1</cite> on endoglucanase 3 from ''Humicola insolens'', and follow a classical [[Koshland double-displacement mechanism]]. No detailed studies involving both steady state and pre-steady state kinetic have yet been reported for this family.

== Catalytic Residues ==
The [[catalytic nucleophile]] of GH family 12 enzymes was initialy predicted by sequence homology to the xylanase members of GH family 11, a glycoside hydrolase family where the [[catalytic nucleophile]] was first identified in the ''Bacillus circulans'' [[endo]]-xylanase through trapping of the 2-deoxy-2-fluoroxylobiosyl-enzyme [[intermediate]] and subsequent peptide mapping via LC-MS/MS technologies <cite>2</cite>. GH family 11 and 12 form together GH clan C of related GH families. The predicted [[catalytic nucleophile]] was subsequentialy confirmed through

== Three-dimensional structures ==
Content is to be added here.

== Family Firsts ==
;First sterochemistry determination: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>11</cite>.
;First catalytic nucleophile identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>12</cite>.
;First general acid/base residue identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>13</cite>.
;First 3-D structure: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>14</cite>.

== References ==
<biblio>
#1 pmid=8223652
#3 pmid=7911679

</biblio>

[[Category:Glycoside Hydrolase Families|GH012]]

Glycoside Hydrolase Family 12

2010-04-05T19:54:27Z

Mats Sandgren:

{{UnderConstruction}}
* [[Author]]: ^^^Mats Sandgren^^^
* [[Responsible Curator]]: ^^^Mats Sandgren^^^
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GHnn'''
|-
|'''Clan'''
|GH-C
|-
|'''Mechanism'''
|retaining
|-
|'''Active site residues'''
|known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |http://www.cazy.org/fam/GH12.html
|}
</div>


== Substrate specificities ==
The substrate specificities found among the [[glycoside hydrolases]] of family 12 are: ''[[endo]]''-β-1,4-glucanase (EC 3.2.1.4), ''xyloglucan hydrolase (EC 3.2.1.151), ''[[endo]]''-β-1,3-1,4-glucanase (EC 3.2.1.73), and xyloglucan ''[[endo]]''-transglycosylase (EC 2.4.1.207),.

== Kinetics and Mechanism ==
GH Family 12 enzymes are [[retaining]] enzymes, as first shown by NMR studdies <cite>1</cite> on endoglucanase 3 from ''Humicola insolens'', and follow a classical [[Koshland double-displacement mechanism]]. No detailed studies involving both steady state and pre-steady state kinetic have yet been reported for this family.

== Catalytic Residues ==
The [[catalytic nucleophile]] of GH family 12 enzymes was initialy predicted by sequence homology to the xylanase members of GH family 11, a glycoside hydrolase family where the [[catalytic nucleophile]] was first identified in the ''Bacillus circulans'' [[endo]]-xylanase through trapping of the 2-deoxy-2-fluoroxylobiosyl-enzyme [[intermediate]] and subsequent peptide mapping via LC-MS/MS technologies <cite>3</cite>.

== Three-dimensional structures ==
Content is to be added here.

== Family Firsts ==
;First sterochemistry determination: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>Comfort2007</cite>.
;First catalytic nucleophile identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>MikesClassic</cite>.
;First general acid/base residue identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>He1999</cite>.
;First 3-D structure: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>3</cite>.

== References ==
<biblio>
#1 pmid=8223652
#3 pmid=7911679

</biblio>

[[Category:Glycoside Hydrolase Families|GH012]]

Glycoside Hydrolase Family 12

2010-04-05T19:26:54Z

Mats Sandgren:

{{UnderConstruction}}
* [[Author]]: ^^^Mats Sandgren^^^
* [[Responsible Curator]]: ^^^Mats Sandgren^^^
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GHnn'''
|-
|'''Clan'''
|GH-C
|-
|'''Mechanism'''
|retaining
|-
|'''Active site residues'''
|known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |http://www.cazy.org/fam/GH12.html
|}
</div>


== Substrate specificities ==
The substrate specificities found among the [[glycoside hydrolases]] of family 12 are: ''[[endo]]''-β-1,4-glucanase (EC 3.2.1.4), ''xyloglucan hydrolase (EC 3.2.1.151), ''[[endo]]''-β-1,3-1,4-glucanase (EC 3.2.1.73), and xyloglucan ''[[endo]]''-transglycosylase (EC 2.4.1.207),.

== Kinetics and Mechanism ==
GH Family 12 enzymes are [[retaining]] enzymes, as first shown by NMR studdies <cite>Schou1993</cite> of endoglucanase 3 from ''Humicola insolens'', and follow a classical [[Koshland double-displacement mechanism]]. No detailed studies involving both steady state and pre-steady state kinetic have jet been reported for this family.

== Catalytic Residues ==
Content is to be added here.

== Three-dimensional structures ==
Content is to be added here.

== Family Firsts ==
;First sterochemistry determination: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>Comfort2007</cite>.
;First catalytic nucleophile identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>MikesClassic</cite>.
;First general acid/base residue identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>He1999</cite>.
;First 3-D structure: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>3</cite>.

== References ==
<biblio>
#Schou1993 pmid=8223652

</biblio>

[[Category:Glycoside Hydrolase Families|GH012]]

Glycoside Hydrolase Family 12

2010-03-05T11:17:31Z

Mats Sandgren:

{{UnderConstruction}}
* [[Author]]: ^^^Mats Sandgren^^^
* [[Responsible Curator]]: ^^^Mats Sandgren^^^
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GHnn'''
|-
|'''Clan'''
|GH-C
|-
|'''Mechanism'''
|retaining
|-
|'''Active site residues'''
|known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |http://www.cazy.org/fam/GH12.html
|}
</div>


== Substrate specificities ==
The substrate specificities found among the [[glycoside hydrolases]] of family 12 are: ''[[endo]]''-β-1,4-glucanase (EC 3.2.1.4), ''xyloglucan hydrolase (EC 3.2.1.151), ''[[endo]]''-β-1,3-1,4-glucanase (EC 3.2.1.73), and xyloglucan ''[[endo]]''-transglycosylase (EC 2.4.1.207),.

References that are not in PubMed can be typed in by hand <cite>MikesClassic</cite>.

== Kinetics and Mechanism ==
Content is to be added here.

== Catalytic Residues ==
Content is to be added here.

== Three-dimensional structures ==
Content is to be added here.

== Family Firsts ==
;First sterochemistry determination: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>Comfort2007</cite>.
;First catalytic nucleophile identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>MikesClassic</cite>.
;First general acid/base residue identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>He1999</cite>.
;First 3-D structure: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>3</cite>.

== References ==
<biblio>
#Comfort2007 pmid=17323919
#He1999 pmid=9312086
#3 isbn=978-0-240-52118-3
#MikesClassic Sinnott, M.L. (1990) Catalytic mechanisms of enzymic glycosyl transfer. Chem. Rev. 90, 1171-1202. [http://dx.doi.org/10.1021/cr00105a006 DOI: 10.1021/cr00105a006]

</biblio>

[[Category:Glycoside Hydrolase Families|GH012]]

Glycoside Hydrolase Family 12

2010-03-05T10:41:28Z

Mats Sandgren:

{{UnderConstruction}}
* [[Author]]: ^^^Mats Sandgren^^^
* [[Responsible Curator]]: ^^^Mats Sandgren^^^
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GHnn'''
|-
|'''Clan'''
|GH-C
|-
|'''Mechanism'''
|retaining
|-
|'''Active site residues'''
|known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |http://www.cazy.org/fam/GH12.html
|}
</div>


== Substrate specificities ==
Content is to be added here.

This is an example of how to make references to a journal article <cite>Comfort2007</cite>. (See the References section below). Multiple references can go in the same place like this <cite>Comfort2007 He1999</cite>. You can even cite books using just the ISBN <cite>3</cite>. References that are not in PubMed can be typed in by hand <cite>MikesClassic</cite>.

== Kinetics and Mechanism ==
Content is to be added here.

== Catalytic Residues ==
Content is to be added here.

== Three-dimensional structures ==
Content is to be added here.

== Family Firsts ==
;First sterochemistry determination: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>Comfort2007</cite>.
;First catalytic nucleophile identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>MikesClassic</cite>.
;First general acid/base residue identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>He1999</cite>.
;First 3-D structure: Cite some reference here, with a ''short'' (1-2 sentence) explanation <cite>3</cite>.

== References ==
<biblio>
#Comfort2007 pmid=17323919
#He1999 pmid=9312086
#3 isbn=978-0-240-52118-3
#MikesClassic Sinnott, M.L. (1990) Catalytic mechanisms of enzymic glycosyl transfer. Chem. Rev. 90, 1171-1202. [http://dx.doi.org/10.1021/cr00105a006 DOI: 10.1021/cr00105a006]

</biblio>

[[Category:Glycoside Hydrolase Families|GH012]]

User:Mats Sandgren

2010-03-03T22:18:12Z

Mats Sandgren: Created page with ' Normal 0 0 1 351 2004 Uppsala Universitet 16 4 2461 11.1287 0 21 0 0 '''This is the user page of Mats Sandgren, Ph.D.''…'

Normal 0 0 1 351 2004 Uppsala Universitet 16 4 2461 11.1287 0 21 0 0

'''This is the user page of Mats Sandgren, Ph.D.'''

I am a faculty scientist at the Department of Molecular Biology at the Swedish university of Agricultural Sciences in Uppsala, Sweden. Together with Associate Professor Jerry Stålberg I am leading a research group where we study structure and function of carbohydrate-active enzymes, with particular interest in plant biomass degrading enzymes used for various biofuel and biorefinery applications.

I am an author and responsible curator of the GH12 page of CAZypedia. If you wish to get in touch with me regarding any issue, involving my authorship of the GH12 page of CAZypedia, send me an e-mail to the following e-mail address: mats.sandgren@molbio.slu.Se

'''Short Curriculum vitae'''

I was borne in Stockholm, Sweden, and was during half of my childhood raised in Sweden and the other half in different countries in Africa. I obtained my MSc degree in biotechnology at the Swedish University of Agricultural (1994). This was followed by a Ph.D degree in molecular biology at Uppsala University (2003), in the group of Prof. Alwyn Jones. My PhD thesis work was focused on determining the three dimensional structure of various GH family12 enzymes by X-ray crystallography. After earning my PhD, I stayed an additional four years at Uppsala University, having a junior faculty position there. During these four years I started to build up a new research group, which I was coordinating the research work within and heading. The research focus of the group was, and still is, structure function studies of various glycoside hydrolases, with a particular focus on structure function studies of various plant-degrading enzymes from the soft rot fungus Hypocrea jecorina. During the summer of 2007 the whole research group moved from Uppsala University to the Department of Molecular Biology at The Swedish university of Agricultural Sciences, where I now have a faculty scientist position. At the new department the group joined forces with the research group of Jerry Ståhlberg. The main research focus of the joint research group is structure function studies of glycoside hydrolases, with a particular focus on proteins from GH family 3, 5,6.7, 16 and 61. Lately the group has also started carrying out different types of application testing studies using the various carbohydrate degrading enzymes studied within the group. These application studies are all carried out within the framework for a thematic research program called MicroDrivE (Microbial Derived Energy) at The Swedish University of Agricultural Sciences, which currently I am the vice program director of.