Carbohydrate Binding Module Family 4 - Revision history

Harry Brumer: Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

2021-12-18T21:18:11Z

Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

Elizabeth Ficko-Blean at 16:00, 6 March 2018

2018-03-06T16:00:47Z

Harry Gilbert: /* Family Firsts */

2018-02-19T14:22:30Z

Family Firsts

Harry Gilbert: /* References */

2018-02-19T14:20:37Z

References

Harry Gilbert: /* Functionalities */

2018-02-19T14:16:01Z

Functionalities

Harry Gilbert: /* Ligand specificities */

2018-02-07T12:40:18Z

Ligand specificities

Harry Gilbert: /* Structural Features */

2018-02-07T11:06:57Z

Structural Features

Harry Gilbert: /* Structural Features */

2018-02-07T11:03:32Z

Structural Features

Harry Gilbert: /* Structural Features */

2018-02-07T09:50:24Z

Structural Features

Harry Gilbert: /* Structural Features */

2018-02-07T09:43:45Z

Structural Features

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 <!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption -->
 {{CuratorApproved}}
-* [[Author]]: ^^^Claire Dumon^^^ and ^^^Harry Gilbert^^^
+* [[Author]]: [[User:Claire Dumon|Claire Dumon]] and [[User:Harry Gilbert|Harry Gilbert]]
-* [[Responsible Curator]]:  ^^^Harry Gilbert^^^
+* [[Responsible Curator]]:  [[User:Harry Gilbert|Harry Gilbert]]
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 CBM4 is a bacterial family of protein modules that comprise around 150 amino acids. CBM4 modules target primarily xylans <cite>Abou-Hachem2000</cite>, &beta;1,3-glucans <cite>Boraston2001</cite> or &beta;1,4-glucans <cite>Tomme1996</cite>, although they all display a degree of non-specific binding to other glycans. For example CBM4 members that primarily target xylan or &beta;1,4-glucan also bind to &beta;1,3-&beta;1,4-mixed linked glucans. CBM4s that target predominantly &beta;1,3-glucans also recognise &beta;1,6-glucans <cite>Zverlov2001</cite>. It should also be emphasised that the CBM4 members that preferentially target &beta;1,4-glucans interact with amorphous cellulose but do not bind to crystalline cellulose <cite>Coutinho1992</cite>.
-CBM4 modules derived from thermophilic and mesophilic bacteria display affinities (K<sub>D</sub> at room temperature) for their primary ligands of ~1 and ~50 &micro;M, respectively <cite>Boraston2001</cite>. When measured, binding for non-primary ligands was ~100-fold lower than for the target glycan <cite>Abou-Hachem2000</cite>. The affinity for oligosaccharides increased up to the pentaose suggesting the presence of five major sugar binding sites <cite>Abou-Hachem2000,Boraston2001,Johnson1996a</cite>.  In all cases isothermal titration calorimetry showed that ligand binding was enthalpically driven and coverage of polysaccharides at saturation indicated an endo-binding mode <cite>Abou-Hachem2000,Boraston2001</cite>. Thus CBM4 is a type B family.
+CBM4 modules derived from thermophilic and mesophilic bacteria display affinities (K<sub>D</sub> at room temperature) for their primary ligands of ~1 and ~50 &micro;M, respectively <cite>Boraston2001</cite>. When measured, binding for non-primary ligands was ~100-fold lower than for the target glycan <cite>Abou-Hachem2000</cite>. The affinity for oligosaccharides increased up to the pentaose suggesting the presence of five major sugar binding sites <cite>Abou-Hachem2000,Boraston2001,Johnson1996a</cite>.  In all cases isothermal titration calorimetry showed that ligand binding was enthalpically driven and coverage of polysaccharides at saturation indicated an endo-binding mode <cite>Abou-Hachem2000,Boraston2001</cite>. Thus CBM4 is a [[Carbohydrate-binding_modules#Types|type B]] family.
 == Structural Features ==

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 == Family Firsts ==
 ;First Identified: The N-terminal CBM4 module (CfCBM4-1)from the ''Cellulomonas fimi'' endoglucanase CenC <cite>Coutinho1992</cite>.
-;First Structural Characterization: The first structural characterization of a CBM4 member was a solution NMR structure of CfCBM4-1 <cite>Johnson1996b</cite>. The first crystal structures of a CBM, and the first ligand complex of this family were CfCBM4-1 and TmCBM4-2 <cite>Boraston2002</cite>.
+;First Structural Characterization: The first structural characterization of a CBM4 member was a solution NMR structure of CfCBM4-1 <cite>Johnson1996b</cite>. The first crystal structures of a CBM, and the first ligand complex of this family were CfCBM4-1 1 with cellopentaose and TmCBM4-2 with laminariheptaose <cite>Boraston2002</cite>.
 == References ==
 <biblio>

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 #Dvortsov2012 Dvortsov, I. A., Lunina, N. A., Zverlov, V. V., and Velikodvorskaya, G. A. (2012)Properties of four C-terminal carbohydrate-binding modules (CBM4) of laminarinase Lic16A of
 Clostridium thermocellum Molecular Biology 46, 817-822 https://doi.org/10.1134/S0026893312060039
 #Gunnarsson2006a Gunnarsson, L. C., Karlsson, E. N., Andersson, M., Holst, O., and Ohlin, M. (2006) Molecular engineering of a thermostable carbohydrate-binding module. Biocatalysis and Biotransformation 24, 31-37 https://doi.org/10.1080/10242420500518516

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 == Functionalities ==
-CBM4 members are located in a variety of xylanases and endo-acting glucanases that target &beta;1,3, mixed linked &beta;1,3-&beta;1,4 or &beta;1,4 linkages. In general the specificity of CBM4s are consistent with the substrate specificity of the enzyme, although the four CBM4 modules in a GH16 laminarinase from ''Clostridium thermocellum'' (CtLic16A) bound to a wide range of glycans, many of which share little structural similarities <cite>Dvortsov2012</cite>. An interesting example of this functional relationship between enzyme activity and the specificity of the CBM module is found in a GH16 laminarinase that displays activity against &beta;1,3-glucan (3G) and mixed linked &beta;1,3-&beta;1,4 glucan (34G), consistent with the preference of the N-terminal CBM4 species for 3G and the C-terminal CBM4 module for 34G <cite>Zverlov2001</cite>. The only evidence of increased affinity of CBM4 members through avidity effects is in CtLic16A <cite>Dvortsov2012</cite>.
+CBM4 members are located in a variety of xylanases and endo-acting glucanases that target &beta;1,3, mixed linked &beta;1,3-&beta;1,4 or &beta;1,4 linkages. In general the specificity of CBM4s are consistent with the substrate specificity of the enzyme, although the four CBM4 modules in a GH16 laminarinase from ''Clostridium thermocellum'' (CtLic16A) bound to a wide range of glycans, many of which share little structural similarities <cite>Dvortsov2012</cite>. An interesting example of this functional relationship between enzyme activity and the specificity of the CBM module is found in a GH16 laminarinase that displays activity against &beta;1,3-glucan (3G) and mixed linked &beta;1,3-&beta;1,4 glucan (34G), consistent with the preference of the N-terminal CBM4 species for 3G and the C-terminal CBM4 module for 34G <cite>Zverlov2001</cite>. The only evidence of increased affinity of CBM4 members through avidity effects is in CtLic16A <cite>Dvortsov2012</cite>. CBM4 modules can also be inserted into the GH10 xylanase catalytic module of Bacteroidetes enzymes <cite>Flint1997</cite>, and this architecture is thought to play a role in enzyme specificity <cite>Dodd2011</cite>. These specific xylanases are mainly encoded by ''Bacteroides'' polysaccharide utilization loci that orchestrate xylan degradation, and these enzymes were suggested to be a functional marker of the utilization of this polysaccharide in the gut <cite>Despres2016</cite>.
 Using phage display of random mutant libraries a range of specificities have been introduced into the RmCBM4-2 scaffold <cite>Gunnarsson2006a</cite>. In addition to generating variants with increased specificity for xylan <cite>Cicortas-Gunnarsson2007</cite>, mutants of the CBM4 module were produced that bound specifically to xyloglucan <cite>von-Schantz2009,Gunnarsson2006b</cite> and even the protein component of immunoglobulins <cite>Gunnarsson2006c</cite>. The engineered RmCBM4-2 variants  have been used to probe plant cell wall architectures <cite>Sandquist2010,Filonova2007</cite>.

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 A crystal structure of a mutant of RmCBM4-2 (X-2; see the Functionalities Section below) in complex with xylopentaose was determined. The data revealed the polar and apolar interactions between the CBM and its ligand, and broadly supports predictions made previously <cite>von-Schantz2012</cite>.  Selection of xylan, rather than soluble &beta;1,4-glucans reflects the requirement for a ligand with a precise 3-fold helical conformation, adopted by xylans, as opposed to the twisted orientation of the corresponding glucose polymers. Apart from one C6 hydroxymethyl group, which caused an important arginine to adopt two conformations, the substantial reduction in affinity for gluco-configured ligands was not the result of steric clashes. The structure of an engineered RmCBM4-2 with increased affinity for xyloglucan compared to the wild type protein has been subjected to extensive structural studies <cite>Fisher2015,von-Schantz2012,Gullfot2010</cite>. One of these studies, using neutron crystallography, revealed increased affinity for xyloglucan through the introduction of polar interactions with the xylose side chains of the ligand <cite>Fisher2015</cite>.
-The NMR structure of the N-terminal CBM4 module (CfCBM4-1) of the ''Cellulomonas fimi'' endoglucanase CenC <cite>Johnson1996b</cite> revealed a similar &beta;-jelly-roll fold to RmCBM4-2. The location of the ligand binding site comprised the concave surface of &beta;-sheet 1 and aromatic and polar residues involved in ligand recognition were proposed.  Crystal structures of TmCBM4-2 and CfCBM4-1, which bind primarily to &beta;1,3-glucan and &beta;1,4-glucan, respectively, were determined in complex with appropriate pentaose ligands <cite>Boraston2002</cite>. The location of the ligand binding cleft, in common with the xylan binding RmCBM4-2, comprises &beta;-sheet 1. A constellation of three aromatic residues, conserved in the two CBMs, form a central aromatic cradle that plays a central role in ligand recognition. These apolar contacts are augmented by a small number of hydrogen bonds with similarly conserved polar amino acids. The importance of these residues was confirmed by spectroscopic and mutagenesis data <cite>Boraston2001,Johnson1996a</cite>. Ligand specificity is conferred by the linear and U-shaped clefts of CfCBM4-1 and TmCBM4-2, respectively, that present optimal topographies for the conformations adopted by &beta;1,4 and &beta;1,3 glucans.
+The NMR structure of the N-terminal CBM4 module (CfCBM4-1) of the ''Cellulomonas fimi'' endoglucanase CenC <cite>Johnson1996b</cite> revealed a similar &beta;-jelly-roll fold to RmCBM4-2. The location of the ligand binding site comprised the concave surface of &beta;-sheet 1 and aromatic and polar residues involved in ligand recognition were proposed.  Crystal structures of TmCBM4-2 and CfCBM4-1, which bind primarily to &beta;1,3-glucan and &beta;1,4-glucan, respectively, were determined in complex with appropriate pentaose ligands <cite>Boraston2002</cite>. The location of the ligand binding cleft, consistent with the NMR structures for RmCBM4-2 and CfCBM4-1, comprises &beta;-sheet 1. A constellation of three aromatic residues, conserved in the two CBMs, form a central aromatic cradle that plays a central role in ligand recognition. These apolar contacts are augmented by a small number of hydrogen bonds with similarly conserved polar amino acids. The importance of these residues was confirmed by spectroscopic and mutagenesis data <cite>Boraston2001,Johnson1996a</cite>. Ligand specificity is conferred by the linear and U-shaped clefts of CfCBM4-1 and TmCBM4-2, respectively, that present optimal topographies for the conformations adopted by &beta;1,4 and &beta;1,3 glucans.
 == Functionalities ==

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 [[File:TMCBM4CFCBM4.png|thumb|300px|right|'''Figure 1.'''  The U shaped conformation of the binding site of TmCBM4 ([{{PDBlink}}1GUI PDB ID 1GUI]) which binds &beta;1,3-glucan and the linear cleft of CfCBM4 ([{{PDBlink}}1GU3 PDB ID 1GU3]) that recognizes &beta;1,4-glucans. The conserved aromatic resisdues are shown in magenta and the carbon of the ligands in green.]]
-The solution 3D structure of the C-terminal CBM4 module (RmCBM4-2) of the ''Rhodothermus marinus'' xylanase Xyn10A was determined by NMR <cite>Simpson2002</cite>. RmCBM4-2 displays a classical β-jelly-roll fold consisting of two β-sheets comprising five (β-sheet 1) and six (β-sheets) anti-parallel b strands, respectively (Figure 1). RmCBM4-2 is particularly thermostable with a Tm of 97 <sup>o</sup>C which is, in part, caused by two structural calcium ions. The location of the high affinity metal binding site is conserved in all CBMs with a &beta;-sandwich fold <cite>Boraston2004</cite>, while the low affinity calcium site <cite>Abou-Hachem2002</cite> has not been reported in any other family of CBMs. The ligand binding cleft is located on the concave surface (β-sheet 1) and changes in the chemical shifts of NMR spectra upon oligosaccharide titrations indicated that two aromatic residues and a number of polar amino acids interact with ligand in its 3-fold screw-axis conformation <cite>Simpson2002</cite>.
+The solution 3D structure of the C-terminal CBM4 module (RmCBM4-2) of the ''Rhodothermus marinus'' xylanase Xyn10A was determined by NMR <cite>Simpson2002</cite>. RmCBM4-2 displays a classical β-jelly-roll fold consisting of two β-sheets comprising five (β-sheet 1) and six (β-sheets) anti-parallel b strands, respectively (Figure 1). RmCBM4-2 is particularly thermostable with a Tm of 97 <sup>o</sup>C which is, in part, caused by two structural calcium ions <cite>Abou-Hachem2002</cite>. The location of the high affinity metal binding site is conserved in all CBMs with a &beta;-sandwich fold <cite>Boraston2004</cite>, while the low affinity calcium site <cite>Abou-Hachem2002</cite> has not been reported in any other family of CBMs. The ligand binding cleft is located on the concave surface (β-sheet 1) and changes in the chemical shifts of NMR spectra upon titrations with xylohexaose indicated that two aromatic residues and a number of polar amino acids interact with ligand in its 3-fold screw-axis conformation <cite>Simpson2002</cite>.
 A crystal structure of a mutant of RmCBM4-2 (X-2; see the Functionalities Section below) in complex with xylopentaose was determined. The data revealed the polar and apolar interactions between the CBM and its ligand, and broadly supports predictions made previously <cite>von-Schantz2012</cite>.  Selection of xylan, rather than soluble &beta;1,4-glucans reflects the requirement for a ligand with a precise 3-fold helical conformation, adopted by xylans, as opposed to the twisted orientation of the corresponding glucose polymers. Apart from one C6 hydroxymethyl group, which caused an important arginine to adopt two conformations, the substantial reduction in affinity for gluco-configured ligands was not the result of steric clashes. The structure of an engineered RmCBM4-2 with increased affinity for xyloglucan compared to the wild type protein has been subjected to extensive structural studies <cite>Fisher2015,von-Schantz2012,Gullfot2010</cite>. One of these studies, using neutron crystallography, revealed increased affinity for xyloglucan through the introduction of polar interactions with the xylose side chains of the ligand <cite>Fisher2015</cite>.