Carbohydrate Binding Module Family 82 - Revision history

Harry Brumer: Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

2021-12-18T21:14:59Z

Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

Elizabeth Ficko-Blean at 06:15, 14 May 2019

2019-05-14T06:15:03Z

Darrell Cockburn at 20:31, 13 May 2019

2019-05-13T20:31:02Z

Elizabeth Ficko-Blean: /* Ligand specificities */

2019-05-13T08:50:17Z

Ligand specificities

Elizabeth Ficko-Blean: /* Family Firsts */

2019-05-13T08:38:03Z

Family Firsts

Elizabeth Ficko-Blean at 08:33, 13 May 2019

2019-05-13T08:33:27Z

Darrell Cockburn at 17:57, 9 May 2019

2019-05-09T17:57:28Z

Nicole Koropatkin at 16:44, 9 May 2019

2019-05-09T16:44:09Z

Darrell Cockburn at 15:25, 9 May 2019

2019-05-09T15:25:51Z

Darrell Cockburn at 15:25, 9 May 2019

2019-05-09T15:25:11Z

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 <!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption -->
 {{CuratorApproved}}
-* [[Author]]: ^^^Darrell Cockburn^^^
+* [[Author]]: [[User:Darrell Cockburn|Darrell Cockburn]]
-* [[Responsible Curator]]:  ^^^Nicole Koropatkin^^^
+* [[Responsible Curator]]:  [[User:Nicole Koropatkin|Nicole Koropatkin]]
 ----

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 [[File:Amy13K_CBM82_highlight.jpg|thumb|600px|right|'''Figure 1. Domain architecture of ''E. rectale'' Amy13K''' The CBM and GH families are noted. The 'S' indicate the signal sequence and the 'anchor' is a cell wall anchor region. The unknown domain has no known function, however, deletion eliminates enzyme activity <cite>Cockburn2018</cite>.]]
-The founding member of this family and the first module to be characterized is the first CBM found in the cell-wall anchored Amy13K from ''Eubacterium rectale'' (see Fig1). It was found to bind beta-cyclodextrin and glycogen with similar affinity, with slightly weaker affinity for maltoheptaose as determined by isothermal titration calorimetry. The module was also found to bind to corn starch granules, both from a wild-type source and from a high amylose source (HiMaize 260) with approximately equal affinity but did not demonstrate binding to potato starch or a chemically crosslinked starch (Fibersym) as determined via depletion assays. Binding to amylopectin and pullulan was also demonstrated via affinity electrophoresis.
+The founding member of this family and the first module to be characterized is the first CBM found in the cell-wall anchored [[GH13]] enzyme Amy13K from ''Eubacterium rectale'' (see Fig1). It was found to bind beta-cyclodextrin and glycogen with similar affinity, with slightly weaker affinity for maltoheptaose as determined by isothermal titration calorimetry. The module was also found to bind to corn starch granules, both from a wild-type source and from a high amylose source (HiMaize 260) with approximately equal affinity but did not demonstrate binding to potato starch or a chemically crosslinked starch (Fibersym) as determined via depletion assays. Binding to amylopectin and pullulan was also demonstrated via affinity electrophoresis.
 <cite>Cockburn2018</cite>.

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 == Structural Features ==
-Secondary structure analysis and alignments suggest they are likely to be beta-sandwich type folds similar to the [[CBM41]] family.
+Secondary structure analysis and alignments suggest they are likely to be beta-sandwich type folds similar to the [[CBM41]] family <cite>Cockburn2018</cite>.
 == Functionalities ==
-This family has been found to be exclusively associated with [[GH13]] family amylases from a quite narrow taxonomic range within ''Roseburia'' and ''Eubacterium rectale''. Removal of the only CBM82 from the ''E. rectale'' Amy13K enzyme resulted in an approximately 2-fold decrease in activity of the enzyme towards amylopectin or potato starch, but resulted in a larger 5-fold decrease in the activity of the enzyme towards corn starch granules, suggesting an important role in targeting the enzyme to this substrate. <cite>Cockburn2018</cite>
+This family has been found to be exclusively associated with [[GH13]] family amylases from a quite narrow taxonomic range within ''Roseburia'' and ''Eubacterium rectale'' <cite>Cockburn2018</cite>. Removal of the only CBM82 from the ''E. rectale'' Amy13K enzyme resulted in an approximately 2-fold decrease in activity of the enzyme towards amylopectin or potato starch, but resulted in a larger 5-fold decrease in the activity of the enzyme towards corn starch granules, suggesting an important role in targeting the enzyme to this substrate. <cite>Cockburn2018</cite>
 == Family Firsts ==

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 == Ligand specificities ==
-[[File:Amy13K_CBM82_highlight.jpg|thumb|600px|right|'''Figure 1. Domain architecture of E. rectale Amy13K''' The CBM and GH families are noted. The 'S' indicate the signal sequence and the 'anchor' is a cell wall anchor region. The unknown domain has no known function, however, deletion eliminates enzyme activity <cite>Cockburn2018</cite>.]]
+[[File:Amy13K_CBM82_highlight.jpg|thumb|600px|right|'''Figure 1. Domain architecture of ''E. rectale'' Amy13K''' The CBM and GH families are noted. The 'S' indicate the signal sequence and the 'anchor' is a cell wall anchor region. The unknown domain has no known function, however, deletion eliminates enzyme activity <cite>Cockburn2018</cite>.]]
 The founding member of this family and the first module to be characterized is the first CBM found in the cell-wall anchored Amy13K from ''Eubacterium rectale'' (see Fig1). It was found to bind beta-cyclodextrin and glycogen with similar affinity, with slightly weaker affinity for maltoheptaose as determined by isothermal titration calorimetry. The module was also found to bind to corn starch granules, both from a wild-type source and from a high amylose source (HiMaize 260) with approximately equal affinity but did not demonstrate binding to potato starch or a chemically crosslinked starch (Fibersym) as determined via depletion assays. Binding to amylopectin and pullulan was also demonstrated via affinity electrophoresis.

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 == Family Firsts ==
-;First Identified
+;First Identified: In ''Roseburia inulinivorans'' as a predicted CBM <cite>Ramsay2006</cite>
 == References ==

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 <!-- This is the end of the table -->
 [[File:Amy13K_CBM82_highlight.jpg|thumb|750px|right|'''Figure 1. Domain architecture of E. rectale Amy13K''' The CBM and GH families are noted. The 'S' indicate the signal sequence and the 'anchor' is a cell wall anchor region. The unknown domain has no known function, however, deletion eliminates enzyme activity <cite>Cockburn2018</cite>.]]
 <br>
 == Ligand specificities ==