Brian Mark: '''Figure 3. Overlay of barley β-glucan exohydrolase isoenzyme ExoI with other known X-ray structures of multi-domain b-glucosidases'''. While additional domains may be present (''not shown for clarity''), the core two-domain architectu...

2013-04-10T19:27:20Z

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'''Figure 3. Overlay of barley β-glucan exohydrolase isoenzyme ExoI with other known X-ray structures of multi-domain b-glucosidases'''. While additional domains may be present (''not shown for clarity''), the core two-domain architecture observed for Exo1 and the unusual two-domain active site architecture (Asp nucleophile on domain 1 and general acid/base on domain 2) appears to be a structurally conserved feature of multidomain GH3 β-glucanases. ''Colour coding'': Exo1 domain 1 (magenta) and domain 2 (cyan) bound to Thiocellobiose (salmon) (PDB: 1IEX); ''Pseudoalteromonas sp. ''Exo-1,3/1,4-b-glucanase ExoP (grey) (PDB: 3UTO); ''Thermotoga neapolitan'' β-glucosidase 3B Bgl3B (orange) (PDB: 2X41); ''Kluyveromyces marxianus ''β-glucosidase KmBgl1 (yellow) (PDB: 3ACO); ''Hypocrea jecorina ''β-glucosidase Bgl1 (green) (PDB:3ZYZ); ''Streptomyces venezuelae'' b-glucosidase DesR (blue) (PDB: 4I3G).

File:GH3 2013 Fig3.png - Revision history

Brian Mark: '''Figure 3. Overlay of barley β-glucan exohydrolase isoenzyme ExoI with other known X-ray structures of multi-domain b-glucosidases'''. While additional domains may be present (''not shown for clarity''), the core two-domain architectu...