Glycoside Hydrolase Family 138 - Revision history

Harry Brumer: Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

2021-12-18T21:14:12Z

Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

Spencer Williams at 21:52, 16 February 2018

2018-02-16T21:52:37Z

Spencer Williams at 21:52, 16 February 2018

2018-02-16T21:52:27Z

Harry Brumer at 08:59, 14 February 2018

2018-02-14T08:59:27Z

Harry Gilbert at 17:24, 13 February 2018

2018-02-13T17:24:07Z

Harry Gilbert at 17:21, 13 February 2018

2018-02-13T17:21:57Z

Harry Gilbert at 17:19, 13 February 2018

2018-02-13T17:19:00Z

Didier Ndeh at 23:16, 1 February 2018

2018-02-01T23:16:23Z

Didier Ndeh at 18:31, 20 January 2018

2018-01-20T18:31:26Z

Harry Brumer at 23:38, 18 January 2018

2018-01-18T23:38:11Z

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 {{CuratorApproved}}
-* [[Author]]: ^^^Didier Ndeh^^^
+* [[Author]]: [[User:Didier Ndeh|Didier Ndeh]]
-* [[Responsible Curator]]:  ^^^Harry Gilbert^^^
+* [[Responsible Curator]]:  [[User:Harry Gilbert|Harry Gilbert]]
 ----

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 == Substrate specificities ==
-[[Glycoside hyddrolases]] of family 138 exhibit α-D-galacturonidase activity. This is based on data from the characterisation of the founding member of the family BT0997 encoded by the human gut bacterium ''B. thetaiotaomicron'' <cite>Ndeh2017</cite>. BT0997 hydrolyses a fragment (GalA-α1,2(GalA-β1,3)(2MeXyl-α1,3-Fuc-α1,4)Rha-&alpha;1,3-Api) of chain A from the pectic polysaccharide rhamnogalacturonan II, producing D-galacturonic acid and the resulting oligosaccharide GalA-β1,3(2MeXyl-α1,3Fuc-α1,4)Rha-α1,3Api <cite>Ndeh2017</cite>.  Several members of this family have been identified in gut and environmental bacteria with a majority of the encoding microbes belonging to the Bacteroidetes phylum <cite>Lombard2014 Cantarel2009</cite>. This phylum is highly represented in human gut microbial populations <cite>Qin2010</cite>.
+[[Glycoside hydrolases]] of family 138 exhibit α-D-galacturonidase activity. This is based on data from the characterisation of the founding member of the family BT0997 encoded by the human gut bacterium ''B. thetaiotaomicron'' <cite>Ndeh2017</cite>. BT0997 hydrolyses a fragment (GalA-α1,2(GalA-β1,3)(2MeXyl-α1,3-Fuc-α1,4)Rha-&alpha;1,3-Api) of chain A from the pectic polysaccharide rhamnogalacturonan II, producing D-galacturonic acid and the resulting oligosaccharide GalA-β1,3(2MeXyl-α1,3Fuc-α1,4)Rha-α1,3Api <cite>Ndeh2017</cite>.  Several members of this family have been identified in gut and environmental bacteria with a majority of the encoding microbes belonging to the Bacteroidetes phylum <cite>Lombard2014 Cantarel2009</cite>. This phylum is highly represented in human gut microbial populations <cite>Qin2010</cite>.
 == Kinetics and Mechanism ==

@@ Line 28: / Line 28: @@
 == Substrate specificities ==
-Members of GH138 exhibit α-D-galacturonidase activity. This is based on data from the characterisation of the founding member of the family BT0997 encoded by the prominent human gut bacterium ''B. thetaiotaomicron'' <cite>Ndeh2017</cite>. BT0997 hydrolyses a fragment (GalA-α1,2(GalA-β1,3)(2MeXyl-α1,3-Fuc-α1,4)Rha-&alpha;1,3-Api) of Chain A from the pectic polysaccharide Rhamnogalacturonan II, producing D-galacturonic acid and the resulting oligosaccharide GalA-β1,3(2MeXyl-α1,3Fuc-α1,4)Rha-α1,3Api <cite>Ndeh2017</cite>, demonstrating that BT0997 cleaved the D-Gal-&alpha;1,2-Rhap linkage.  Several members of this family have been identified in gut and environmental bacteria with a majority of the encoding microbes belonging to the Bacteroidetes phylum <cite>Lombard2014 Cantarel2009</cite>. This phylum is highly represented in human gut microbial populations <cite>Qin2010</cite>.
+[[Glycoside hyddrolases]] of family 138 exhibit α-D-galacturonidase activity. This is based on data from the characterisation of the founding member of the family BT0997 encoded by the human gut bacterium ''B. thetaiotaomicron'' <cite>Ndeh2017</cite>. BT0997 hydrolyses a fragment (GalA-α1,2(GalA-β1,3)(2MeXyl-α1,3-Fuc-α1,4)Rha-&alpha;1,3-Api) of chain A from the pectic polysaccharide rhamnogalacturonan II, producing D-galacturonic acid and the resulting oligosaccharide GalA-β1,3(2MeXyl-α1,3Fuc-α1,4)Rha-α1,3Api <cite>Ndeh2017</cite>.  Several members of this family have been identified in gut and environmental bacteria with a majority of the encoding microbes belonging to the Bacteroidetes phylum <cite>Lombard2014 Cantarel2009</cite>. This phylum is highly represented in human gut microbial populations <cite>Qin2010</cite>.
 == Kinetics and Mechanism ==
@@ Line 44: / Line 44: @@
 ;First general acid/base residue identification:Currently unknown.
 ;First 3-D structure:Currently unknown.
 == References ==
 <biblio>

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 {{CuratorApproved}}
 * [[Author]]: ^^^Didier Ndeh^^^
@@ Line 29: / Line 28: @@
 == Substrate specificities ==
-Glycoside hydrolases of family 138 (GH138) exhibit α-D-galacturonidase activity. This is based on data from the characterisation of the founding member of the family BT0997 encoded by the prominent human gut bacterium ''B. thetaiotaomicron'' <cite>Ndeh2017</cite>. BT0997 hydrolyses a fragment (GalA-α1,2(GalA-β1,3)(2MeXyl-α1,3-Fuc-α1,4)Rha-&alpha;1,3-Api) of Chain A from the pectic polysaccharide Rhamnogalacturonan II, producing D-galacturonic acid and the resulting oligosaccharide GalA-β1,3(2MeXyl-α1,3Fuc-α1,4)Rha-α1,3Api <cite>Ndeh2017</cite>, demonstrating that BT0997 cleaved the D-Gal-&alpha;1,2-Rhap linkage.  Several members of this family have been identified in gut and environmental bacteria with a majority of the encoding microbes belonging to the Bacteroidetes phylum <cite>Lombard2014 Cantarel2009</cite>. This phylum is highly represented in human gut microbial populations <cite>Qin2010</cite>.
+Members of GH138 exhibit α-D-galacturonidase activity. This is based on data from the characterisation of the founding member of the family BT0997 encoded by the prominent human gut bacterium ''B. thetaiotaomicron'' <cite>Ndeh2017</cite>. BT0997 hydrolyses a fragment (GalA-α1,2(GalA-β1,3)(2MeXyl-α1,3-Fuc-α1,4)Rha-&alpha;1,3-Api) of Chain A from the pectic polysaccharide Rhamnogalacturonan II, producing D-galacturonic acid and the resulting oligosaccharide GalA-β1,3(2MeXyl-α1,3Fuc-α1,4)Rha-α1,3Api <cite>Ndeh2017</cite>, demonstrating that BT0997 cleaved the D-Gal-&alpha;1,2-Rhap linkage.  Several members of this family have been identified in gut and environmental bacteria with a majority of the encoding microbes belonging to the Bacteroidetes phylum <cite>Lombard2014 Cantarel2009</cite>. This phylum is highly represented in human gut microbial populations <cite>Qin2010</cite>.
 == Kinetics and Mechanism ==
-The kinetic mechanism for this family has not been reported
+The kinetic mechanism for this family has not been reported.
 == Catalytic Residues ==
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 == Three-dimensional structures ==
-No 3D structure for a member of this family has been currently reported
+No 3D structure for a member of this family has been currently reported.
 == Family Firsts ==

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 <!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption -->
-{{UnderConstruction}}
+{{CuratorApproved}}
 * [[Author]]: ^^^Didier Ndeh^^^
 * [[Responsible Curator]]:  ^^^Harry Gilbert^^^
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 == Substrate specificities ==
-Glycoside hydrolases of family 138 (GH138) exhibit α-D-galacturonidase activity. This is based on data from the characterisation of the founding member of the family BT0997 encoded by the prominent human gut bacterium ''B. thetaiotaomicron'' <cite>Ndeh2017</cite>. BT0997 hydrolyses a fragment (GalA-α1,2(GalA-β1,3)(2MeXyl-α1,3-Fuc-α1,4)Rha-&alpha;1,3-Api) of Chain A from the pectic polysaccharide Rhamnogalacturonan II, producing D-galacturonic acid and the resulting oligosaccharide GalA-β1,3(2MeXylα1,3Fucα1,4)Rha-α1,3Api <cite>Ndeh2017</cite>, demonstrating that BT0997 cleaved the D-Gal-&alpha;1,2-Rhap linkage.  Several members of this family have been identified in gut and environmental bacteria with a majority of the encoding microbes belonging to the Bacteroidetes phylum <cite>Lombard2014 Cantarel2009</cite>. This phylum is highly represented in human gut microbial populations <cite>Qin2010</cite>.
+Glycoside hydrolases of family 138 (GH138) exhibit α-D-galacturonidase activity. This is based on data from the characterisation of the founding member of the family BT0997 encoded by the prominent human gut bacterium ''B. thetaiotaomicron'' <cite>Ndeh2017</cite>. BT0997 hydrolyses a fragment (GalA-α1,2(GalA-β1,3)(2MeXyl-α1,3-Fuc-α1,4)Rha-&alpha;1,3-Api) of Chain A from the pectic polysaccharide Rhamnogalacturonan II, producing D-galacturonic acid and the resulting oligosaccharide GalA-β1,3(2MeXyl-α1,3Fuc-α1,4)Rha-α1,3Api <cite>Ndeh2017</cite>, demonstrating that BT0997 cleaved the D-Gal-&alpha;1,2-Rhap linkage.  Several members of this family have been identified in gut and environmental bacteria with a majority of the encoding microbes belonging to the Bacteroidetes phylum <cite>Lombard2014 Cantarel2009</cite>. This phylum is highly represented in human gut microbial populations <cite>Qin2010</cite>.
 == Kinetics and Mechanism ==

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 <biblio>
 #Cantarel2009 pmid=18838391
 #Ndeh2017 pmid=28329766
 #Qin2010 pmid=20203603