Glycoside Hydrolase Family 139 - Revision history

Harry Brumer: Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

2021-12-18T21:20:39Z

Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

Spencer Williams at 21:45, 16 February 2018

2018-02-16T21:45:07Z

Harry Brumer at 09:00, 14 February 2018

2018-02-14T09:00:07Z

Harry Gilbert at 17:28, 13 February 2018

2018-02-13T17:28:19Z

Harry Gilbert at 17:27, 13 February 2018

2018-02-13T17:27:50Z

Ana Luis at 20:41, 11 February 2018

2018-02-11T20:41:16Z

Ana Luis at 20:27, 11 February 2018

2018-02-11T20:27:47Z

Ana Luis at 20:23, 11 February 2018

2018-02-11T20:23:44Z

Harry Brumer: Created page with " {{UnderConstruct..."

2018-01-08T23:46:09Z

Created page with " {{UnderConstruct..."

New page

{{UnderConstruction}}
* [[Author]]: ^^^Ana Luis^^^
* [[Responsible Curator]]: ^^^Harry Gilbert^^^
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GH139'''
|-
|'''Clan'''
|GH-x
|-
|'''Mechanism'''
|retaining/inverting
|-
|'''Active site residues'''
|known/not known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |{{CAZyDBlink}}GH139.html
|}
</div>


== Substrate specificities ==
Content is to be added here.

Authors may get an idea of what to put in each field from ''Curator Approved'' [[Glycoside Hydrolase Families]]. ''(TIP: Right click with your mouse and open this link in a new browser window...)''

In the meantime, please see these references for an essential introduction to the CAZy classification system: <cite>DaviesSinnott2008 Cantarel2009</cite>.

== Kinetics and Mechanism ==
Content is to be added here.

== Catalytic Residues ==
Content is to be added here.

== Three-dimensional structures ==
Content is to be added here.

== Family Firsts ==
;First stereochemistry determination: Content is to be added here.
;First catalytic nucleophile identification: Content is to be added here.
;First general acid/base residue identification: Content is to be added here.
;First 3-D structure: Content is to be added here.

== References ==
<biblio>
#Cantarel2009 pmid=18838391
#DaviesSinnott2008 Davies, G.J. and Sinnott, M.L. (2008) Sorting the diverse: the sequence-based classifications of carbohydrate-active enzymes. ''The Biochemist'', vol. 30, no. 4., pp. 26-32. [http://www.biochemist.org/bio/03004/0026/030040026.pdf Download PDF version].
</biblio>

[[Category:Glycoside Hydrolase Families|GH139]]

@@ Line 28: / Line 28: @@
 == Substrate specificities ==
-Glycoside hydrolases of the bacterial GH139 family display the novel α-2-O-methyl-L-fucosidase activity. The enzyme BT0984 from ''Bacteroides thetaiotaomicron'' is the only member of this family that has been characterized. This exo-enzyme targets the 2-O-methyl-L-fucose-&alpha;-1,2-D-Galp linkage in chain B of the complex pectin rhamnogalacturonan-II <cite>Ndeh2017</cite>.
+[[Glycoside hydrolases]] of family 139 family display α-2-''O''-methyl-L-fucosidase activity. They are exclusively bacterial in origin. The enzyme BT0984 from ''Bacteroides thetaiotaomicron'' is the only member of this family that has been characterized. This ''exo''-acting enzyme targets the 2-''O''-methyl-L-fucose-&alpha;-1,2-D-Galp linkage in chain B of the complex pectin rhamnogalacturonan-II <cite>Ndeh2017</cite>.
 == Kinetics and Mechanism ==
-The stereochemistry of the reaction catalyzed by GH139 members has not yet been studied.
+The stereochemistry of the reaction catalyzed by GH139 members has not yet been reported.
 == Catalytic Residues ==

@@ Line 29: / Line 29: @@
 == Substrate specificities ==
-Glycoside hydrolases of GH139 family ([http://www.cazy.org/GH139.html CAZy]) display the novel α-2-O-methyl-L-fucosidase activity. The enzyme BT0984 from ''Bacteroides thetaiotaomicron'' is the only member of this family that has been characterized. This exo-enzyme targets the α-2-O-methyl-L-fucose at chain B of the complex pectin rhamnogalacturonan-II <cite>Ndeh2017</cite>. All of genes encoding family 139 members are found in bacteria.
+Glycoside hydrolases of the bacterial GH139 family ([http://www.cazy.org/GH139.html CAZy]) display the novel α-2-O-methyl-L-fucosidase activity. The enzyme BT0984 from ''Bacteroides thetaiotaomicron'' is the only member of this family that has been characterized. This exo-enzyme targets the 2-O-methyl-L-fucose-&alpha;-1,2-D-Galp linkage in chain B of the complex pectin rhamnogalacturonan-II <cite>Ndeh2017</cite>.
 == Kinetics and Mechanism ==
-The stereochemistry of the reaction catalyzed by GH129 members has not yet been studied.
+The stereochemistry of the reaction catalyzed by GH139 members has not yet been studied.
 == Catalytic Residues ==

← Older revision		Revision as of 20:27, 11 February 2018
Line 29:		Line 29:

	== Substrate specificities ==		== Substrate specificities ==
		+	Glycoside hydrolases of GH139 family ([www.cazy.org/GH139.html CAZy]) display the novel a-2-O-methyl-L-fucosidase activity (EC…….). The enzyme BT0984 from Bacteroides thetaiotaomicron is the only member of this family that has been characterized. This exo-enzyme targets the a-2-O-methyl-L-fucose at chain B of the complex rhamnogalacturonan-II present in the plants cell wall. All of genes encoding family 139 members are found in bacteria.
		+
	Content is to be added here.		Content is to be added here.

@@ Line 1: / Line 1: @@
 {{CuratorApproved}}
-* [[Author]]: ^^^Ana Luis^^^
+* [[Author]]: [[User:Ana Luis|Ana Luis]]
-* [[Responsible Curator]]:  ^^^Harry Gilbert^^^
+* [[Responsible Curator]]:  [[User:Harry Gilbert|Harry Gilbert]]
 ----

@@ Line 29: / Line 29: @@
 == Substrate specificities ==
-Glycoside hydrolases of GH139 family ([www.cazy.org/GH139.html CAZy]) display the novel a-2-O-methyl-L-fucosidase activity (EC…….). The enzyme BT0984 from Bacteroides thetaiotaomicron is the only member of this family that has been characterized. This exo-enzyme targets the a-2-O-methyl-L-fucose at chain B of the complex rhamnogalacturonan-II present in the plants cell wall. All of genes encoding family 139 members are found in bacteria.
+Glycoside hydrolases of GH139 family ([http://www.cazy.org/GH139.html CAZy]) display the novel α-2-O-methyl-L-fucosidase activity. The enzyme BT0984 from ''Bacteroides thetaiotaomicron'' is the only member of this family that has been characterized. This exo-enzyme targets the α-2-O-methyl-L-fucose at chain B of the complex pectin rhamnogalacturonan-II <cite>Ndeh2017</cite>. All of genes encoding family 139 members are found in bacteria.
 == Kinetics and Mechanism ==
-Content is to be added here.
+The stereochemistry of the reaction catalyzed by GH129 members has not yet been studied.
 == Catalytic Residues ==
-Content is to be added here.
+Not known.
 == Three-dimensional structures ==
-Content is to be added here.
+No three-dimensional structure has been solved for this family.
 == Family Firsts ==
-;First stereochemistry determination: Content is to be added here.
+;First stereochemistry determination: Unknown.
-;First catalytic nucleophile identification: Content is to be added here.
+;First catalytic nucleophile identification: Unknown.
-;First general acid/base residue identification: Content is to be added here.
+;First general acid/base residue identification: Unknown.
-;First 3-D structure: Content is to be added here.
+;First 3-D structure: Unknown.
 == References ==
 <biblio>
-#Cantarel2009 pmid=18838391
+#Ndeh2017 pmid=28329766
 </biblio>
 [[Category:Glycoside Hydrolase Families|GH139]]

@@ Line 12: / Line 12: @@
 |-
 |'''Clan'''
-|GH-x
+|none
 |-
 |'''Mechanism'''
-|retaining/inverting
+|unknown
 |-
 |'''Active site residues'''
-|known/not known
+|not known
 |-
 |{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''