Glycoside Hydrolase Family 142 - Revision history

Grete Raba at 22:12, 25 August 2023

2023-08-25T22:12:01Z

Grete Raba at 22:11, 25 August 2023

2023-08-25T22:11:36Z

Grete Raba at 21:49, 25 August 2023

2023-08-25T21:49:13Z

Grete Raba at 20:34, 25 August 2023

2023-08-25T20:34:25Z

Grete Raba at 19:43, 25 August 2023

2023-08-25T19:43:56Z

Grete Raba at 19:37, 25 August 2023

2023-08-25T19:37:37Z

Grete Raba at 19:19, 25 August 2023

2023-08-25T19:19:23Z

Grete Raba at 19:16, 25 August 2023

2023-08-25T19:16:42Z

Grete Raba at 19:15, 25 August 2023

2023-08-25T19:15:48Z

Grete Raba at 19:13, 25 August 2023

2023-08-25T19:13:00Z

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 |-
 |'''Mechanism'''
-|unknown
+|not known
 |-
 |'''Active site residues'''

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 |-
 |'''Mechanism'''
-|retaining/inverting
+|unknown
 |-
 |'''Active site residues'''
-|known/not known
+|not known
 |-
 |{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''

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 == Substrate specificities ==
-The glycoside hydrolases from GH family 142 have β-L-arabinofuranosidase ([{{EClink}}3.2.1.185 EC 3.2.1.185]) activity. The first characterized enzyme from GH142 was the C-terminus of BT1020 from ''Bacteroides thetaiotaomicron'' <cite>Ndeh2017</cite>. BT1020 hydrolyses the β-1,5 linkage between L-arabinofuranosidase and D-DHA in the terminal region of rhamnogalacturonan II (RG II) D chain found in pectin.
+The glycoside hydrolases from GH family 142 have β-L-arabinofuranosidase ([{{EClink}}3.2.1.185 EC 3.2.1.185]) activity. The first characterized enzyme from GH142 was the C-terminus of BT1020 from ''Bacteroides thetaiotaomicron'' <cite>Ndeh2017</cite>. BT1020 C-terminus hydrolyses the β-1,5 linkage between L-arabinofuranosidase and D-DHA at the non-reducing end of rhamnogalacturonan II (RG II) D chain found in pectin.
 == Kinetics and Mechanism ==

@@ Line 29: / Line 29: @@
 == Substrate specificities ==
-The glycoside hydrolases from GH family 142 have β-L-arabinofuranosidase (EC 3.2.1.185) activity. The first characterized enzyme from GH142 was the C-terminus of BT1020 from ''Bacteroides thetaiotaomicron'' <cite>Ndeh2017</cite>. BT1020 hydrolyses the β-1,5 linkage between L-arabinofuranosidase and D-DHA in the terminal region of rhamnogalacturonan II (RG II) D chain found in pectin.
+The glycoside hydrolases from GH family 142 have β-L-arabinofuranosidase ([{{EClink}}3.2.1.185 EC 3.2.1.185]) activity. The first characterized enzyme from GH142 was the C-terminus of BT1020 from ''Bacteroides thetaiotaomicron'' <cite>Ndeh2017</cite>. BT1020 hydrolyses the β-1,5 linkage between L-arabinofuranosidase and D-DHA in the terminal region of rhamnogalacturonan II (RG II) D chain found in pectin.
 == Kinetics and Mechanism ==

@@ Line 29: / Line 29: @@
 == Substrate specificities ==
-The glycoside hydrolases from GH family 142 have β-L-arabinofuranosidase (EC 3.2.1.185) activity. The first characterized enzyme from GH142 was the C-terminus of BT1020 from ''Bacteroides thetaiotaomicron'' <ref>Ndeh2017</ref>. BT1020 hydrolyses the β-1,5 linkage between L-arabinofuranosidase and D-DHA in the terminal region of rhamnogalacturonan II (RG II) D chain found in pectin.
+The glycoside hydrolases from GH family 142 have β-L-arabinofuranosidase (EC 3.2.1.185) activity. The first characterized enzyme from GH142 was the C-terminus of BT1020 from ''Bacteroides thetaiotaomicron'' <cite>Ndeh2017</cite>. BT1020 hydrolyses the β-1,5 linkage between L-arabinofuranosidase and D-DHA in the terminal region of rhamnogalacturonan II (RG II) D chain found in pectin.
 == Kinetics and Mechanism ==
@@ Line 35: / Line 35: @@
 == Catalytic Residues ==
-The β-L-arabinofuranosidase from BT1020 C-terminus contains canonical glycoside hydrolase catalytic apparatus comprising carboxylate residues<ref>Ndeh2017</ref>.
+The β-L-arabinofuranosidase from BT1020 C-terminus contains canonical glycoside hydrolase catalytic apparatus comprising carboxylate residues <cite>Ndeh2017</cite>.
 == Three-dimensional structures ==
-Content is to be added here.
+The β-L-arabinofuranosidase from the C-terminus of BT1020 has a (α/α)6-barrel structure <cite>Ndeh2017</cite>.
 == Family Firsts ==
-;First stereochemistry determination: Content is to be added here.
+;First stereochemistry determination: Not yet identified.
-;First catalytic nucleophile identification: Content is to be added here.
+;First catalytic nucleophile identification: BT1020 from ''Bacteroides thetaiotaomicron'' <cite>Ndeh2017</cite>.
-;First general acid/base residue identification: Content is to be added here.
+;First general acid/base residue identification: Not yet identified.
-;First 3-D structure: Content is to be added here.
+;First 3-D structure: BT1020 from ''Bacteroides thetaiotaomicron'' <cite>Ndeh2017</cite>.
 == References ==

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 == References ==
 <biblio>
-#Ndeh2017(pmid=28329766).
+#Ndeh2017 pmid=28329766
 </biblio>
 [[Category:Glycoside Hydrolase Families|GH142]]