Glycoside Hydrolase Family 147 - Revision history

Harry Brumer: Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

2021-12-18T21:15:45Z

Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

Kazune Tamura: /* Kinetics and Mechanism */

2019-02-23T23:34:41Z

Kinetics and Mechanism

Spencer Williams at 21:31, 16 February 2018

2018-02-16T21:31:32Z

Harry Gilbert at 18:51, 15 February 2018

2018-02-15T18:51:19Z

Harry Gilbert at 18:50, 15 February 2018

2018-02-15T18:50:31Z

Jonathon Briggs at 15:10, 18 January 2018

2018-01-18T15:10:29Z

Harry Brumer: Created page with " {{UnderConstruct..."

2018-01-10T18:33:58Z

Created page with " {{UnderConstruct..."

New page

{{UnderConstruction}}
* [[Author]]: ^^^Jonathon Briggs^^^
* [[Responsible Curator]]: ^^^Harry Gilbert^^^
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GH147'''
|-
|'''Clan'''
|GH-x
|-
|'''Mechanism'''
|retaining/inverting
|-
|'''Active site residues'''
|known/not known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |{{CAZyDBlink}}GH147.html
|}
</div>


== Substrate specificities ==
Content is to be added here.

Authors may get an idea of what to put in each field from ''Curator Approved'' [[Glycoside Hydrolase Families]]. ''(TIP: Right click with your mouse and open this link in a new browser window...)''

In the meantime, please see these references for an essential introduction to the CAZy classification system: <cite>DaviesSinnott2008 Cantarel2009</cite>.

== Kinetics and Mechanism ==
Content is to be added here.

== Catalytic Residues ==
Content is to be added here.

== Three-dimensional structures ==
Content is to be added here.

== Family Firsts ==
;First stereochemistry determination: Content is to be added here.
;First catalytic nucleophile identification: Content is to be added here.
;First general acid/base residue identification: Content is to be added here.
;First 3-D structure: Content is to be added here.

== References ==
<biblio>
#Cantarel2009 pmid=18838391
#DaviesSinnott2008 Davies, G.J. and Sinnott, M.L. (2008) Sorting the diverse: the sequence-based classifications of carbohydrate-active enzymes. ''The Biochemist'', vol. 30, no. 4., pp. 26-32. [http://www.biochemist.org/bio/03004/0026/030040026.pdf Download PDF version].
</biblio>

[[Category:Glycoside Hydrolase Families|GH147]]

@@ Line 32: / Line 32: @@
 == Kinetics and Mechanism ==
-<sup>1</sup>NMR analysis of the galactose product released from BACOVA_05493 action on galactotriose revealed cleavage with [[retention|retaining]] of anomeric stereochemistry <cite>Luis2017</cite>. The enzyme is believed to operate through a [[classical Koshland double-displacement mechanism]].
+<sup>1</sup>NMR analysis of the galactose product released from BACOVA_05493 action on galactotriose revealed cleavage with retention of anomeric stereochemistry <cite>Luis2017</cite>. The enzyme is believed to operate through a [[classical Koshland double-displacement mechanism]].
 == Catalytic Residues ==

@@ Line 29: / Line 29: @@
 == Substrate specificities ==
-The founding member of GH147, BACOVA_05493 from ''Bacteroides ovatus'' is a β1,4-galactosidase. The enzyme demonstrates a preference towards longer oligosaccharides and &beta;1,4-galactan, releasing galactopyranose from the oligosaccharide or polysaccharide chain. BACOVA_05493 is unable to hydrolyse galactobiose <cite>Luis2017</cite>.
+The founding member of [[glycoside hydrolase]] family 147, BACOVA_05493 from ''Bacteroides ovatus'' is a β1,4-galactosidase. The enzyme demonstrates a preference towards longer oligosaccharides and &beta;1,4-galactan, releasing galactopyranose from the oligosaccharide or polysaccharide chain. BACOVA_05493 is unable to hydrolyse β1,4-galactobiose <cite>Luis2017</cite>.
 == Kinetics and Mechanism ==
-NMR analysis of the galactose product released from galactotriose revealed a retaining mechanism of action <cite>Luis2017</cite>.
+<sup>1</sup>NMR analysis of the galactose product released from BACOVA_05493 action on galactotriose revealed cleavage with [[retention|retaining]] of anomeric stereochemistry <cite>Luis2017</cite>. The enzyme is believed to operate through a [[classical Koshland double-displacement mechanism]].
 == Catalytic Residues ==
-Hydrophobic cluster analysis showed that BACOVA_05493 is a member of Clan GH-A. Based on this assumption the catalytic nucleophile and general acid/base residues of the founding member of GH147 were proposed as Glu300 and Glu203, respectively. This hypothesis was supported by mutagenesis data showing that the E300A and E203A mutants were catalytically inactive <cite>Luis2017</cite>.
+Hydrophobic cluster analysis showed that BACOVA_05493 is a member of Clan GH-A. Based on this assumption the catalytic nucleophile and general acid/base residues were proposed as Glu300 and Glu203, respectively. This hypothesis was supported by mutagenesis data showing that the E300A and E203A mutants were catalytically inactive <cite>Luis2017</cite>.
 == Three-dimensional structures ==

@@ Line 29: / Line 29: @@
 == Substrate specificities ==
-The founding member of GH147, BACOVA_05493 a β1,4-galactosidase demonstrates a preference towards longer oligosaccharides releasing galacopyranose from the oligosaccharide chain. BACOVA_ is unable to hydrolyse galactobiose <cite>Luis2017</cite>.
+The founding member of GH147, BACOVA_05493 from ''Bacteroides ovatus'' is a β1,4-galactosidase. The enzyme demonstrates a preference towards longer oligosaccharides and &beta;1,4-galactan, releasing galactopyranose from the oligosaccharide or polysaccharide chain. BACOVA_05493 is unable to hydrolyse galactobiose <cite>Luis2017</cite>.
 == Kinetics and Mechanism ==
-NMR analysis of the hydrolysis product revealed a retaining mechanism of action <cite>Luis2017</cite>.
+NMR analysis of the galactose product released from galactotriose revealed a retaining mechanism of action <cite>Luis2017</cite>.
 == Catalytic Residues ==
-The catalytic nucleophile and general acid/base residues of the founding member of GH147, BACOVA_05493, were identified as Glu300 and Glu203, respectively <cite>Luis2017</cite>.
 == Three-dimensional structures ==
-Currently there is no crystal structure of any member of GH147.
 == Family Firsts ==
-;First stereochemistry determination:     BACOVA_05493 from B. ovatus <cite>Luis2017</cite>.
+;First stereochemistry determination:  BACOVA_05493 from ''B. ovatus'' was shown to have a retaining mechanism <cite>Luis2017</cite>.
-;First catalytic nucleophile identification:     BACOVA_05493 from B. ovatus <cite>Luis2017</cite>.
+;First catalytic nucleophile identification:  BACOVA_05493 from ''B. ovatus'' <cite>Luis2017</cite>.
-;First general acid/base residue identification:     BACOVA_05493 from B. ovatus <cite>Luis2017</cite>.
+;First general acid/base residue identification:   BACOVA_05493 from ''B. ovatus'' <cite>Luis2017</cite>.
-;First 3-D structure: Currently not available.
 == References ==

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 <!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption -->
 {{CuratorApproved}}
-* [[Author]]: ^^^Jonathon Briggs^^^
+* [[Author]]: [[User:Jonathon Briggs|Jonathon Briggs]]
-* [[Responsible Curator]]:  ^^^Harry Gilbert^^^
+* [[Responsible Curator]]:  [[User:Harry Gilbert|Harry Gilbert]]
 ----

@@ Line 12: / Line 12: @@
 |-
 |'''Clan'''
-|GH-x
+|GH-A
 |-
 |'''Mechanism'''
-|retaining/inverting
+|retaining
 |-
 |'''Active site residues'''
-|known/not known
+|known
 |-
 |{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
@@ Line 29: / Line 29: @@
 == Substrate specificities ==
-Content is to be added here.
+The founding member of GH147, BACOVA_05493 a β1,4-galactosidase demonstrates a preference towards longer oligosaccharides releasing galacopyranose from the oligosaccharide chain. BACOVA_ is unable to hydrolyse galactobiose <cite>Luis2017</cite>.
 == Kinetics and Mechanism ==
-Content is to be added here.
+NMR analysis of the hydrolysis product revealed a retaining mechanism of action <cite>Luis2017</cite>.
 == Catalytic Residues ==
-Content is to be added here.
+The catalytic nucleophile and general acid/base residues of the founding member of GH147, BACOVA_05493, were identified as Glu300 and Glu203, respectively <cite>Luis2017</cite>.
 == Three-dimensional structures ==
-Content is to be added here.
+Currently there is no crystal structure of any member of GH147.
 == Family Firsts ==
-;First stereochemistry determination: Content is to be added here.
+;First stereochemistry determination:     BACOVA_05493 from B. ovatus <cite>Luis2017</cite>.
-;First catalytic nucleophile identification: Content is to be added here.
+;First catalytic nucleophile identification:     BACOVA_05493 from B. ovatus <cite>Luis2017</cite>.
-;First general acid/base residue identification: Content is to be added here.
+;First general acid/base residue identification:     BACOVA_05493 from B. ovatus <cite>Luis2017</cite>.
-;First 3-D structure: Content is to be added here.
+;First 3-D structure: Currently not available.
 == References ==
 <biblio>
-#Cantarel2009 pmid=18838391
+#Luis2017 pmid=29255254
 </biblio>
 [[Category:Glycoside Hydrolase Families|GH147]]