Glycoside Hydrolase Family 187 - Revision history

Yaoguang Chang at 06:46, 5 January 2024

2024-01-05T06:46:10Z

Jingjing Shen at 09:01, 27 December 2023

2023-12-27T09:01:11Z

Jingjing Shen at 08:58, 27 December 2023

2023-12-27T08:58:10Z

Jingjing Shen at 08:56, 27 December 2023

2023-12-27T08:56:21Z

Jingjing Shen at 08:42, 27 December 2023

2023-12-27T08:42:57Z

Jingjing Shen at 08:09, 27 December 2023

2023-12-27T08:09:01Z

Harry Brumer at 19:27, 18 December 2023

2023-12-18T19:27:04Z

Elizabeth Ficko-Blean: Created page with " {{UnderConstruc..."

2023-12-18T14:48:18Z

Created page with "  {{UnderConstruc..."

New page

{{UnderConstruction}}
* [[Author]]: [[User:Jingjing Shen|Jingjing Shen]]
* [[Responsible Curator]]: [[User:Yaoguang Chang|Yaoguang Chang]]
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GH187'''
|-
|'''Clan'''
|GH-x
|-
|'''Mechanism'''
|retaining/inverting
|-
|'''Active site residues'''
|known/not known
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |{{CAZyDBlink}}GHnn.html
|}
</div>


== Substrate specificities ==
Content is to be added here.

Authors may get an idea of what to put in each field from ''Curator Approved'' [[Glycoside Hydrolase Families]]. ''(TIP: Right click with your mouse and open this link in a new browser window...)''

In the meantime, please see these references for an essential introduction to the CAZy classification system: <cite>DaviesSinnott2008 Cantarel2009</cite>.

== Kinetics and Mechanism ==
Content is to be added here.

== Catalytic Residues ==
Content is to be added here.

== Three-dimensional structures ==
Content is to be added here.

== Family Firsts ==
;First stereochemistry determination: Content is to be added here.
;First catalytic nucleophile identification: Content is to be added here.
;First general acid/base residue identification: Content is to be added here.
;First 3-D structure: Content is to be added here.

== References ==
<biblio>
#Cantarel2009 pmid=18838391
#DaviesSinnott2008 Davies, G.J. and Sinnott, M.L. (2008) Sorting the diverse: the sequence-based classifications of carbohydrate-active enzymes. ''The Biochemist'', vol. 30, no. 4., pp. 26-32. [https://doi.org/10.1042/BIO03004026 DOI:10.1042/BIO03004026].
</biblio>


[[Category:Glycoside Hydrolase Families|GH187]]

@@ Line 30: / Line 30: @@
 == Substrate specificities ==
-Members of glycoside hydrolase family 187 have been shown to exhibit α-1,3-L-fucanase activity. The first member of this family, Fun187A from a marine bacterium ''Wenyingzhuangia aestuarii'' OF219, specifically hydrolyzes the α-1,3-L-fucoside bonds between non-sulfated and 2-O-sulfated fucose residuess in the sulfated fucan oligosaccharide α-L-Fuc''p''(2,4OSO<sub>3</sub><sup>-</sup>)-1→3-α-L-Fuc''p''-1→3-α-L-Fuc''p''(2OSO<sub>3</sub><sup>-</sup>)-1→3-α-L-Fuc''p''(2OSO<sub>3</sub><sup>-</sup>) in an endo-acting manner <cite>Shen2024</cite>. Meanwhile, one homologue of Fun187A displays activities toward sulfated fucan from ''Holothuria tubulosa'' and ''Isostichopus badionotus'', namely WP_159020740.1 <cite>Shen2024</cite>.
+Members of glycoside hydrolase family 187 have been shown to exhibit α-1,3-L-fucanase activity. The first member of this family, Fun187A from a marine bacterium ''Wenyingzhuangia aestuarii'' OF219, specifically hydrolyzes the α-1,3-L-fucoside bonds between non-sulfated and 2-O-sulfated fucose residuess in the sulfated fucan oligosaccharide α-L-Fuc''p''(2,4OSO<sub>3</sub><sup>-</sup>)-1→3-α-L-Fuc''p''-1→3-α-L-Fuc''p''(2OSO<sub>3</sub><sup>-</sup>)-1→3-α-L-Fuc''p''(2OSO<sub>3</sub><sup>-</sup>) in an endo-acting manner <cite>Shen2024</cite>. Meanwhile, one homologue of Fun187A displays activities toward sulfated fucans from ''Holothuria tubulosa'' and ''Isostichopus badionotus'', namely WP_159020740.1 <cite>Shen2024</cite>.
 [[File: Figure 1.jpg|thumb|''' Figure 1. The phylogenetic tree of GH187 homologues. Sequences confirmed to exhibit α-1,3-L-fucanase activity were highlighted in red triangles.]]

@@ Line 30: / Line 30: @@
 == Substrate specificities ==
-Members of glycoside hydrolase family 187 have been shown to exhibit α-1,3-L-fucanase activity. The first member of this family, Fun187A from a marine bacterium ''Wenyingzhuangia aestuarii'' OF219, specifically hydrolyzes the α-1,3-L-fucoside bonds between non-sulfated and 2-O-sulfated fucose residuess in the sulfated fucan oligosaccharide α-L-Fucp(2,4OSO<sub>3</sub><sup>-</sup>)-1→3-α-L-Fucp-1→3-α-L-Fucp(2OSO<sub>3</sub><sup>-</sup>)-1→3-α-L-Fucp(2OSO<sub>3</sub><sup>-</sup>) in an endo-acting manner <cite>Shen2024</cite>. Meanwhile, one homologue of Fun187A displays activities toward sulfated fucan from ''Holothuria tubulosa'' and ''Isostichopus badionotus'', namely WP_159020740.1 <cite>Shen2024</cite>.
+Members of glycoside hydrolase family 187 have been shown to exhibit α-1,3-L-fucanase activity. The first member of this family, Fun187A from a marine bacterium ''Wenyingzhuangia aestuarii'' OF219, specifically hydrolyzes the α-1,3-L-fucoside bonds between non-sulfated and 2-O-sulfated fucose residuess in the sulfated fucan oligosaccharide α-L-Fuc''p''(2,4OSO<sub>3</sub><sup>-</sup>)-1→3-α-L-Fuc''p''-1→3-α-L-Fuc''p''(2OSO<sub>3</sub><sup>-</sup>)-1→3-α-L-Fuc''p''(2OSO<sub>3</sub><sup>-</sup>) in an endo-acting manner <cite>Shen2024</cite>. Meanwhile, one homologue of Fun187A displays activities toward sulfated fucan from ''Holothuria tubulosa'' and ''Isostichopus badionotus'', namely WP_159020740.1 <cite>Shen2024</cite>.
 [[File: Figure 1.jpg|thumb|''' Figure 1. The phylogenetic tree of GH187 homologues. Sequences confirmed to exhibit α-1,3-L-fucanase activity were highlighted in red triangles.]]

@@ Line 30: / Line 30: @@
 == Substrate specificities ==
-Members of glycoside hydrolase family 187 have been shown to exhibit α-1,3-L-fucanase activity. The first member of this family, Fun187A from a marine bacterium ''Wenyingzhuangia aestuarii'' OF219, specifically hydrolyzes the α-1,3-L-fucoside bonds between non-sulfated and 2-O-sulfated fucose residuess in the sulfated fucan oligosaccharide α-L-Fucp(2,4OSO3-)-1→3-α-L-Fucp-1→3-α-L-Fucp(2OSO3-)-1→3-α-L-Fucp(2OSO<sub>3</sub><sup>-</sup>) in a endo-acting manner <cite>Shen2024</cite>. Meanwhile, one homologue of Fun187A displays activities toward sulfated fucan from ''Holothuria tubulosa'' and ''Isostichopus badionotus'', namely WP_159020740.1 <cite>Shen2024</cite>.
+Members of glycoside hydrolase family 187 have been shown to exhibit α-1,3-L-fucanase activity. The first member of this family, Fun187A from a marine bacterium ''Wenyingzhuangia aestuarii'' OF219, specifically hydrolyzes the α-1,3-L-fucoside bonds between non-sulfated and 2-O-sulfated fucose residuess in the sulfated fucan oligosaccharide α-L-Fucp(2,4OSO<sub>3</sub><sup>-</sup>)-1→3-α-L-Fucp-1→3-α-L-Fucp(2OSO<sub>3</sub><sup>-</sup>)-1→3-α-L-Fucp(2OSO<sub>3</sub><sup>-</sup>) in an endo-acting manner <cite>Shen2024</cite>. Meanwhile, one homologue of Fun187A displays activities toward sulfated fucan from ''Holothuria tubulosa'' and ''Isostichopus badionotus'', namely WP_159020740.1 <cite>Shen2024</cite>.
 [[File: Figure 1.jpg|thumb|''' Figure 1. The phylogenetic tree of GH187 homologues. Sequences confirmed to exhibit α-1,3-L-fucanase activity were highlighted in red triangles.]]

@@ Line 1: / Line 1: @@
 <!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption -->
-{{UnderConstruction}}
+{{CuratorApproved}}
 * [[Author]]: [[User:Jingjing Shen|Jingjing Shen]]
 * [[Responsible Curator]]:  [[User:Yaoguang Chang|Yaoguang Chang]]

@@ Line 13: / Line 13: @@
 |-
 |'''Clan'''
-|GH-x
+|not known
 |-
 |'''Mechanism'''
-|retaining/inverting
+|not known
 |-
 |'''Active site residues'''
-|known/not known
+|not known
 |-
 |{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
@@ Line 30: / Line 30: @@
 == Substrate specificities ==
-Members of glycoside hydrolase family 187 have been shown to exhibit α-1,3-L-fucanase activity. The first member of this family, Fun187A from a marine bacterium ''Wenyingzhuangia aestuarii'' OF219, specifically hydrolyzes the α-1,3-L-fucoside bonds between non-sulfated and 2-O-sulfated fucose residuess in the sulfated fucan oligosaccharide α-L-Fucp(2,4OSO3-)-1→3-α-L-Fucp-1→3-α-L-Fucp(2OSO3-)-1→3-α-L-Fucp(2OSO<sub>3</sub><sup>-</sup>) in a random endo-acting manner. Meanwhile, one homologue of Fun187A displays activities toward sulfated fucan from Holothuria tubulosa and Isostichopus badionotus, namely WP_159020740.1.
+Members of glycoside hydrolase family 187 have been shown to exhibit α-1,3-L-fucanase activity. The first member of this family, Fun187A from a marine bacterium ''Wenyingzhuangia aestuarii'' OF219, specifically hydrolyzes the α-1,3-L-fucoside bonds between non-sulfated and 2-O-sulfated fucose residuess in the sulfated fucan oligosaccharide α-L-Fucp(2,4OSO3-)-1→3-α-L-Fucp-1→3-α-L-Fucp(2OSO3-)-1→3-α-L-Fucp(2OSO<sub>3</sub><sup>-</sup>) in a endo-acting manner <cite>Shen2024</cite>. Meanwhile, one homologue of Fun187A displays activities toward sulfated fucan from ''Holothuria tubulosa'' and ''Isostichopus badionotus'', namely WP_159020740.1 <cite>Shen2024</cite>.
+[[File: Figure 1.jpg|thumb|''' Figure 1. The phylogenetic tree of GH187 homologues. Sequences confirmed to exhibit α-1,3-L-fucanase activity were highlighted in red triangles.]]
 == Kinetics and Mechanism ==
-The catalytic mechanism of GH187 has not been identified. As mentioned in the report, Fun187A showed no transglycosylating activity in the tested acceptor substrates, such as D-glucose, D-galactose, D-mannose, D-fructose, L-fucose, D-glucosamine, N-acetyl-D-glucosamine, glycerin, and methanol.
+The catalytic mechanism of GH187 has not been identified. As mentioned in the report, Fun187A showed no transglycosylating activity in the tested acceptor substrates, such as D-glucose, D-galactose, D-mannose, D-fructose, L-fucose, D-glucosamine, N-acetyl-D-glucosamine, glycerin, and methanol <cite>Shen2024</cite>.
 == Catalytic Residues ==
@@ Line 53: / Line 50: @@
 == References ==
 <biblio>
-#Cantarel2009 pmid=18838391
+#Shen2024 pmid=37940306
 </biblio>
 <!-- Do not delete this Category tag -->
 [[Category:Glycoside Hydrolase Families|GH187]]

@@ Line 30: / Line 30: @@
 == Substrate specificities ==
-Content is to be added here.
+Members of glycoside hydrolase family 187 have been shown to exhibit α-1,3-L-fucanase activity. The first member of this family, Fun187A from a marine bacterium ''Wenyingzhuangia aestuarii'' OF219, specifically hydrolyzes the α-1,3-L-fucoside bonds between non-sulfated and 2-O-sulfated fucose residuess in the sulfated fucan oligosaccharide α-L-Fucp(2,4OSO3-)-1→3-α-L-Fucp-1→3-α-L-Fucp(2OSO3-)-1→3-α-L-Fucp(2OSO<sub>3</sub><sup>-</sup>) in a random endo-acting manner. Meanwhile, one homologue of Fun187A displays activities toward sulfated fucan from Holothuria tubulosa and Isostichopus badionotus, namely WP_159020740.1.
 Authors may get an idea of what to put in each field from ''Curator Approved'' [[Glycoside Hydrolase Families]]. ''(TIP: Right click with your mouse and open this link in a new browser window...)''
@@ Line 37: / Line 37: @@
 == Kinetics and Mechanism ==
-Content is to be added here.
+The catalytic mechanism of GH187 has not been identified. As mentioned in the report, Fun187A showed no transglycosylating activity in the tested acceptor substrates, such as D-glucose, D-galactose, D-mannose, D-fructose, L-fucose, D-glucosamine, N-acetyl-D-glucosamine, glycerin, and methanol.
 == Catalytic Residues ==
-Content is to be added here.
+No catalytic residues have been identified in this glycoside hydrolase family at present.
 == Three-dimensional structures ==
-Content is to be added here.
+No three-dimensional structure has been solved in this glycoside hydrolase family at present.
 == Family Firsts ==
-;First stereochemistry determination: Content is to be added here.
+;First stereochemistry determination: Not yet identified.
-;First catalytic nucleophile identification: Content is to be added here.
+;First catalytic nucleophile identification: Not yet identified.
-;First general acid/base residue identification: Content is to be added here.
+;First general acid/base residue identification: Not yet identified.
-;First 3-D structure: Content is to be added here.
+;First 3-D structure: Not yet identified.
 == References ==

@@ Line 23: / Line 23: @@
 |{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
 |-
-| colspan="2" |{{CAZyDBlink}}GHnn.html
+| colspan="2" |{{CAZyDBlink}}GH187.html
 |}
 </div>
@@ Line 59: / Line 59: @@
 <!-- Do not delete this Category tag -->
 [[Category:Glycoside Hydrolase Families|GH187]]