Glycoside Hydrolase Family 50 - Revision history

Harry Brumer: Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

2021-12-18T21:14:24Z

Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

Kazune Tamura: /* Kinetics and Mechanism */

2019-02-23T23:28:56Z

Kinetics and Mechanism

Harry Brumer: updated reference identifiers to standard AuthorYear format to make future editing easier

2011-05-27T12:05:38Z

updated reference identifiers to standard AuthorYear format to make future editing easier

Harry Brumer: added external links to CAZy clans

2010-07-01T06:47:00Z

added external links to CAZy clans

Harry Brumer at 06:44, 1 July 2010

2010-07-01T06:44:37Z

Mirjam Czjzek at 14:19, 30 June 2010

2010-06-30T14:19:16Z

Mirjam Czjzek at 14:18, 30 June 2010

2010-06-30T14:18:26Z

Mirjam Czjzek at 14:14, 30 June 2010

2010-06-30T14:14:23Z

Mirjam Czjzek at 14:11, 30 June 2010

2010-06-30T14:11:38Z

Mirjam Czjzek at 20:48, 29 May 2010

2010-05-29T20:48:15Z

@@ Line 1: / Line 1: @@
 <!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption -->
 {{CuratorApproved}}
-* [[Author]]: ^^^Mirjam Czjzek^^^
+* [[Author]]: [[User:Mirjam Czjzek|Mirjam Czjzek]]
-* [[Responsible Curator]]:  ^^^Mirjam Czjzek^^^
+* [[Responsible Curator]]:  [[User:Mirjam Czjzek|Mirjam Czjzek]]
 ----

@@ Line 31: / Line 31: @@
 == Kinetics and Mechanism ==
-That GH50 enzymes potentially utilize a retaining mechanism has only been inferred by analogy with clan [http://www.cazy.org/Glycoside-Hydrolases.html GH-A] enzymes. No mechanistic or kintetic analysis demonstrating the stereochemical outcome of the reaction have been reported for this family to date.
+That GH50 enzymes potentially utilize a retaining mechanism has only been inferred by analogy with clan [http://www.cazy.org/Glycoside-Hydrolases.html GH-A] enzymes. No mechanistic or kinetic analysis demonstrating the stereochemical outcome of the reaction have been reported for this family to date.
 == Catalytic Residues ==

@@ Line 28: / Line 28: @@
 == Substrate specificities ==
-To date, all characterized [[glycoside hydrolases]] of family 50 are &beta;-agarases (EC [{{EClink}}3.2.1.81 3.2.1.81]) that cleave &beta;-1,4 glycosidic bonds of agarose, releasing neoagaro-biose -tetraose and -hexaose <cite>REF1,REF2,REF3,REF4</cite>. Three enzymes, Aga50A and Aga50D from ''Saccharophagus degradans'' and Aga50B from ''Vibrio sp.'' have been reported to be pure exo-&beta;-agarases <cite>REF5</cite>.
+To date, all characterized [[glycoside hydrolases]] of family 50 are &beta;-agarases (EC [{{EClink}}3.2.1.81 3.2.1.81]) that cleave &beta;-1,4 glycosidic bonds of agarose, releasing neoagaro-biose -tetraose and -hexaose <cite>Sugano1993 Sugano1994 Ohta2005 Lee2006</cite>. Three enzymes, Aga50A and Aga50D from ''Saccharophagus degradans'' and Aga50B from ''Vibrio sp.'' have been reported to be pure exo-&beta;-agarases <cite>Kim2010</cite>.
 == Kinetics and Mechanism ==
@@ Line 40: / Line 40: @@
 == Family Firsts ==
-;Identification of first family member: This family was created in the [{{CAZyDBlink}}GH50.html CAZy Ddatabase] following the work of Sugano et al. <cite>REF1</cite>.
+;Identification of first family member: This family was created in the [{{CAZyDBlink}}GH50.html CAZy Ddatabase] following the work of Sugano et al. <cite>Sugano1993</cite>.
 ;First stereochemistry determination: not determined yet.
 ;First catalytic nucleophile identification: not determined yet.
@@ Line 48: / Line 48: @@
 == References ==
 <biblio>
-#REF1 pmid=8517750
+#Sugano1993 pmid=8517750
-#REF2 pmid=8193156
+#Sugano1994 pmid=8193156
-#REF3 pmid=15307821
+#Ohta2005 pmid=15307821
-#REF4 pmid=17028783
+#Lee2006 pmid=17028783
-#REF5 pmid=19802606
+#Kim2010 pmid=19802606
 </biblio>
 [[Category:Glycoside Hydrolase Families|GH050]]

@@ Line 18: / Line 18: @@
 |-
 |'''Active site residues'''
-|inferred from clan GH-A as two Glu
+|inferred from clan [http://www.cazy.org/Glycoside-Hydrolases.html GH-A]<br>as two Glu
 |-
 |{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
@@ Line 31: / Line 31: @@
 == Kinetics and Mechanism ==
-That GH50 enzymes potentially utilize a retaining mechanism has only been inferred by analogy with clan GH-A enzymes. No mechanistic or kintetic analysis demonstrating the stereochemical outcome of the reaction have been reported for this family to date.
+That GH50 enzymes potentially utilize a retaining mechanism has only been inferred by analogy with clan [http://www.cazy.org/Glycoside-Hydrolases.html GH-A] enzymes. No mechanistic or kintetic analysis demonstrating the stereochemical outcome of the reaction have been reported for this family to date.
 == Catalytic Residues ==
-Similarly, the catalytic residues in this family have not been directly identified, but may be inferred from superposition with other clan GH-A enzymes.
+Similarly, the catalytic residues in this family have not been directly identified, but may be inferred from superposition with other clan [http://www.cazy.org/Glycoside-Hydrolases.html GH-A] enzymes.
 == Three-dimensional structures ==
-Unknown; from analogy to clan GH-A enzymes it can be inferred that the 3D structure will be based on a (&beta;/&alpha;)<sub>8</sub> barrel fold.
+Unknown; from analogy to clan [http://www.cazy.org/Glycoside-Hydrolases.html GH-A] enzymes it can be inferred that the 3D structure will be based on a (&beta;/&alpha;)<sub>8</sub> barrel fold.
 == Family Firsts ==

@@ Line 1: / Line 1: @@
 <!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption -->
-{{UnderConstruction}}
+{{CuratorApproved}}
 * [[Author]]: ^^^Mirjam Czjzek^^^
 * [[Responsible Curator]]:  ^^^Mirjam Czjzek^^^
@@ Line 26: / Line 26: @@
 </div>
 <!-- This is the end of the table -->
 == Substrate specificities ==
 To date, all characterized [[glycoside hydrolases]] of family 50 are &beta;-agarases (EC [{{EClink}}3.2.1.81 3.2.1.81]) that cleave &beta;-1,4 glycosidic bonds of agarose, releasing neoagaro-biose -tetraose and -hexaose <cite>REF1,REF2,REF3,REF4</cite>. Three enzymes, Aga50A and Aga50D from ''Saccharophagus degradans'' and Aga50B from ''Vibrio sp.'' have been reported to be pure exo-&beta;-agarases <cite>REF5</cite>.
 == Kinetics and Mechanism ==
-Actually, a potential retaining mechanism of this glycoside hydrolase family can only be inferred from analogy to clan GH-A enzymes {{CAZyDBlink}}GH50.html. No mechanistic or kintetic analysis demonstrating the stereochemical outcome of the reaction have been reported for this family to date.
+That GH50 enzymes potentially utilize a retaining mechanism has only been inferred by analogy with clan GH-A enzymes. No mechanistic or kintetic analysis demonstrating the stereochemical outcome of the reaction have been reported for this family to date.
 == Catalytic Residues ==
-Unkown
+Similarly, the catalytic residues in this family have not been directly identified, but may be inferred from superposition with other clan GH-A enzymes.
 == Three-dimensional structures ==
 Unknown; from analogy to clan GH-A enzymes it can be inferred that the 3D structure will be based on a (&beta;/&alpha;)<sub>8</sub> barrel fold.
 == Family Firsts ==
-;Identification of first family member : The family was created in the Cazy-database following the work of Sugano et al. <cite>REF1</cite>.
+;Identification of first family member: This family was created in the [{{CAZyDBlink}}GH50.html CAZy Ddatabase] following the work of Sugano et al. <cite>REF1</cite>.
 ;First stereochemistry determination: not determined yet.
 ;First catalytic nucleophile identification: not determined yet.
@@ Line 58: / Line 53: @@
 #REF4 pmid=17028783
 #REF5 pmid=19802606
 </biblio>
 [[Category:Glycoside Hydrolase Families|GH050]]

@@ Line 29: / Line 29: @@
 == Substrate specificities ==
-To date, all characterized [[glycoside hydrolases]] of family 50 are &beta;-agarases (EC [{{EClink}}3.2.1.81 3.2.1.81]) that cleave &beta;-1,4 glycosidic bonds of agarose, releasing neoagaro-biose -tetraose and -hexaose <cite>REF1,REF2,REF3</cite>. Three enzymes, Aga50A and Aga50D from ''Saccharophagus degradans'' and Aga50B from ''Vibrio sp.'' have been reported to be pure exo-&beta;-agarases <cite>REF4</cite>.
+To date, all characterized [[glycoside hydrolases]] of family 50 are &beta;-agarases (EC [{{EClink}}3.2.1.81 3.2.1.81]) that cleave &beta;-1,4 glycosidic bonds of agarose, releasing neoagaro-biose -tetraose and -hexaose <cite>REF1,REF2,REF3</cite>. Three enzymes, Aga50A and Aga50D from ''Saccharophagus degradans'' and Aga50B from ''Vibrio sp.'' have been reported to be pure exo-&beta;-agarases <cite>REF5</cite>.
 == Kinetics and Mechanism ==
-Actually, the potentially retaining mechanism of this glycoside hydrolase familly can only be inferred from analogy to clan GH-A enzymes {{CAZyDBlink}}GH50.html. No mechanistic or kintetic analysis demonstrating the stereochemical outcome of the reaction have been reported for this family to date.
+Actually, a potential retaining mechanism of this glycoside hydrolase familly can only be inferred from analogy to clan GH-A enzymes {{CAZyDBlink}}GH50.html. No mechanistic or kintetic analysis demonstrating the stereochemical outcome of the reaction have been reported for this family to date.
 == Catalytic Residues ==
-Content is to be added here.
+Unkown
 == Three-dimensional structures ==
-Content is to be added here.
+Unknown; from analogy to clan GH-A enzymes it can be inferred that the 3D structure will be based on a (&beta;/a)<sub>8</sub> barrel fold.
 == Family Firsts ==
-;First stereochemistry determination: Cite some reference here, with a ''short'' (1-2 sentence) explanation .
+;Identification of first family member : The family was created in Cazy based on the work of XXXX et al. <cite>REF1</cite>.
-;First catalytic nucleophile identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation.
+;First stereochemistry determination: not determined yet.
-;First general acid/base residue identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation.
+;First catalytic nucleophile identification: not determined yet.
-;First 3-D structure: Cite some reference here, with a ''short'' (1-2 sentence) explanation.
+;First general acid/base residue identification: not determined yet.
 == References ==
@@ Line 55: / Line 56: @@
 #REF2 pmid=8193156
 #REF3 pmid=15307821
-#REF4 pmid=19802606
+#REF4 pmid=8193156
 </biblio>