Polysaccharide Lyase Family 20 - Revision history

Harry Brumer: Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

2021-12-18T21:17:49Z

Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

2020-08-06T22:20:54Z

2014-09-13T02:06:00Z

2014-09-13T02:04:46Z

Three-dimensional structures

2014-09-11T04:47:05Z

Substrate specificities

2014-09-11T04:42:02Z

Substrate specificities

2014-09-05T19:48:39Z

2014-09-02T16:48:33Z

2014-09-02T16:34:57Z

2014-09-02T16:33:53Z

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 <!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption -->
 {{CuratorApproved}}
-* [[Author]]: ^^^Naotake Konno^^^
+* [[Author]]: [[User:Naotake Konno|Naotake Konno]]
-* [[Responsible Curator]]:  ^^^Shinya Fushinobu^^^
+* [[Responsible Curator]]:  [[User:Shinya Fushinobu|Shinya Fushinobu]]
 ----

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 == Three-dimensional structures ==
-[[Image:PL20.png|thumb|300px|right|'''Figure 1:''' PL20 glucuronan lyase from ''Trichoderma reesei'' (2ZZJ).]]
+[[Image:PL20.png|thumb|300px|right|'''Figure 1:''' PL20 glucuronan lyase from ''Trichoderma reesei'' (PDB ID [{{PDBlink}}2zzj 2zzj]).]]
 The ligand-free structure of TrGL was the first PL20 structure to be reported ('''Figure 1''', PDB ID [{{PDBlink}}2zzj 2zzj], 1.8 &Aring; resolution) <cite>Konno2009b</cite>. TrGL has a typical &beta;-jelly roll fold. A calcium binding site, which appears to contribute to the stability, was found at a position far from the cleft. However, no calcium binding site in the cleft has been identified.

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 == Three-dimensional structures ==
-[[Image:PL20.png|thumb|400px|right|'''Figure 1:''' PL20 glucuronan lyase from ''Trichoderma reesei'' (2ZZJ).]]
+[[Image:PL20.png|thumb|300px|right|'''Figure 1:''' PL20 glucuronan lyase from ''Trichoderma reesei'' (2ZZJ).]]
 The ligand-free structure of TrGL was the first PL20 structure to be reported ('''Figure 1''', PDB ID [{{PDBlink}}2zzj 2zzj], 1.8 &Aring; resolution) <cite>Konno2009b</cite>. TrGL has a typical &beta;-jelly roll fold. A calcium binding site, which appears to contribute to the stability, was found at a position far from the cleft. However, no calcium binding site in the cleft has been identified.

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 == Three-dimensional structures ==
-[[Image:PL20.png|'''Figure 1:''' PL20 glucuronan lyase from ''Trichoderma reesei'' (2ZZJ).|frame|right]]
+[[Image:PL20.png|thumb|400px|right|'''Figure 1:''' PL20 glucuronan lyase from ''Trichoderma reesei'' (2ZZJ).]]
 The ligand-free structure of TrGL was the first PL20 structure to be reported ('''Figure 1''', PDB ID [{{PDBlink}}2zzj 2zzj], 1.8 &Aring; resolution) <cite>Konno2009b</cite>. TrGL has a typical &beta;-jelly roll fold. A calcium binding site, which appears to contribute to the stability, was found at a position far from the cleft. However, no calcium binding site in the cleft has been identified.

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 == Substrate specificities ==
 Polysaccharide lyases of family 20 cleave &beta;-1,4 linkages in polyglucuronate (&beta;-1,4-glucuronan lyase; EC 4.2.2.14). The first PL20 enzyme was cloned from filamentous fungus ''Trichoderma reesei'' (TrGL) <cite>Konno2009a</cite>. TrGL was highly specific for &beta;-1,4-glucuronan prepared from regenerated cellulose by 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation (cellouronate).
-Polyglucuronate (glucuronan) is a minor polysaccharide compared with other polyuronates. But &beta;-1,4-glucuronan structures are found in water-soluble polysaccharides from bacteria, fungi, and algae.
+Polyglucuronate is a minor polysaccharide compared with other polyuronates. But &beta;-1,4-glucuronan structures are found in water-soluble polysaccharides from bacteria, fungi, and algae.
 == Kinetics and Mechanism ==

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 |-
 |'''Charge neutraliser'''
-|calcium
+|none
 |-
 |'''Active site residues'''

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 |-
 |{{Hl2}} colspan="2" align="center" |'''Polysaccharide Lyase Family PL20'''
 |-
 |'''Mechanism'''

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 |&beta;-elimination
 |-
-|'''Charge Neutraliser'''
+|'''Charge neutraliser'''
 |calcium
 |-