Polysaccharide Lyase Family 30 - Revision history

Harry Brumer: Removed CAZy link in text - see table instead

2024-10-01T23:47:05Z

Removed CAZy link in text - see table instead

Marie-Line Garron at 15:31, 23 August 2024

2024-08-23T15:31:52Z

Marie-Line Garron at 15:27, 23 August 2024

2024-08-23T15:27:16Z

Marie-Line Garron at 13:24, 23 August 2024

2024-08-23T13:24:53Z

Marie-Line Garron at 13:21, 23 August 2024

2024-08-23T13:21:52Z

Marie-Line Garron at 13:16, 23 August 2024

2024-08-23T13:16:03Z

Harry Brumer: Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

2021-12-18T21:19:43Z

Text replacement - "\^\^\^(.*)\^\^\^" to "$1"

Harry Brumer at 01:07, 13 June 2020

2020-06-13T01:07:07Z

Harry Brumer at 01:01, 13 June 2020

2020-06-13T01:01:58Z

Harry Brumer: Created page with " {{UnderConstruct..."

2020-06-13T01:01:41Z

Created page with " {{UnderConstruct..."

New page

{{UnderConstruction}}
* [[Author]]: ^^^Marie-Line Garron^^^
* [[Responsible Curator]]: ^^^Marie-Line Garron^^^
----


<div style="float:right">
{| {{Prettytable}}
|-
|{{Hl2}} colspan="2" align="center" |'''Polysaccharide Lyase Family PL10'''
|-
|'''3D Structure'''
|
|-
|'''Mechanism'''
|
|-
|'''Charge neutraliser'''
|
|-
|'''Active site residues'''
|
|-
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
|-
| colspan="2" |{{CAZyDBlink}}PL30.html
|}
</div>


== Substrate specificities ==
Content is to be added here.

Authors may get an idea of what to put in each field from ''Curator Approved'' [[Polysaccharide Lyase Families]]. ''(TIP: Right click with your mouse and open this link in a new browser window...)''

In the meantime, please see these references for an essential introduction to the CAZy classification system: <cite>DaviesSinnott2008 Cantarel2009</cite>.

== Kinetics and Mechanism ==
Content is to be added here.

== Catalytic Residues ==
Content is to be added here.

== Three-dimensional structures ==
Content is to be added here.

== Family Firsts ==
;First stereochemistry determination: Content is to be added here.
;First catalytic nucleophile identification: Content is to be added here.
;First general acid/base residue identification: Content is to be added here.
;First 3-D structure: Content is to be added here.

== References ==
<biblio>
#Cantarel2009 pmid=18838391
#DaviesSinnott2008 Davies, G.J. and Sinnott, M.L. (2008) Sorting the diverse: the sequence-based classifications of carbohydrate-active enzymes. ''The Biochemist'', vol. 30, no. 4., pp. 26-32. [https://doi.org/10.1042/BIO03004026 Download PDF version].
</biblio>

[[Category:Glycoside Hydrolase Families|PL030]]

@@ Line 32: / Line 32: @@
 == Substrate specificities ==
-PL30 is a small family (38 sequences), exclusively bacterial belonging to the phylum ''Bacteroidetes'' ([http://www.cazy.org/PL30.html PL30]) <cite>Drula2022</cite>. The founding member of family 30, and the only one characterized, belongs to ''Bacteroides cellulosilyticus WH2 (BcellWH2_02524, ''WP_029426181.1). The activity was measured against hyaluronic acid by following the absorbance at 232nm. The increase in absorbance confirms that  ''BcellWH2_02524 ''is a hyaluronan lyase ([https://www.enzyme-database.org/query.php?ec=4.2.2.1 4.2.2.1]), probably using the by &beta;-elimination process like all PL families <cite>Helbert2019</cite>.
+PL30 is a small family (38 sequences), exclusively bacterial belonging to the phylum ''Bacteroidetes''. The founding member of family 30, and the only one characterized, belongs to ''Bacteroides cellulosilyticus WH2 (BcellWH2_02524, ''WP_029426181.1). The activity was measured against hyaluronic acid by following the absorbance at 232nm. The increase in absorbance confirms that  ''BcellWH2_02524 ''is a hyaluronan lyase ([https://www.enzyme-database.org/query.php?ec=4.2.2.1 4.2.2.1]), probably using the by &beta;-elimination process like all PL families <cite>Helbert2019</cite>.
 == Kinetics and Mechanism ==
@@ Line 51: / Line 51: @@
 == References ==
 <biblio>
 #Helbert2019 pmid=30850540
 </biblio>
 [[Category:Polysaccharide Lyase Families|PL030]]

@@ Line 45: / Line 45: @@
 == Family Firsts ==
 ;First description of catalytic activity: Hyaluronan lyase was the first activity discovered for the family PL30 <cite>Helbert2019</cite>.
-;First catalytic base identification: Content is to be added here.
+;First charge neutralizer identification: To be determined
-;First catalytic acid identification: Content is to be added here.
+;First Brønstead acid and base residue identification: To be determinated
-;First 3-D structure: Content is to be added here.
+;First 3-D structure: To be determinated
 == References ==

@@ Line 32: / Line 32: @@
 == Substrate specificities ==
-PL30 is a small family (38 sequences), exclusively bacterial belonging to the phylum ''Bacteroidetes'' ([http://www.cazy.org/PL30.html PL30]) <cite>Drula2022</cite>. The founding member of family 30, and the only one characterized, belongs to ''Bacteroides cellulosilyticus WH2 (BcellWH2_02524, ''WP_029426181.1). The activity was measured against hyaluronic acid by following the absorbance at 232nm. The increase in absorbance confirms that  ''BcellWH2_02524 ''is a hyaluronan lyase ([https://www.enzyme-database.org/query.php?ec=4.2.2.1 4.2.2.1]), probably using the by b-elimination process like all PL families <cite>Helbert2019</cite>.
+PL30 is a small family (38 sequences), exclusively bacterial belonging to the phylum ''Bacteroidetes'' ([http://www.cazy.org/PL30.html PL30]) <cite>Drula2022</cite>. The founding member of family 30, and the only one characterized, belongs to ''Bacteroides cellulosilyticus WH2 (BcellWH2_02524, ''WP_029426181.1). The activity was measured against hyaluronic acid by following the absorbance at 232nm. The increase in absorbance confirms that  ''BcellWH2_02524 ''is a hyaluronan lyase ([https://www.enzyme-database.org/query.php?ec=4.2.2.1 4.2.2.1]), probably using the by &beta;-elimination process like all PL families <cite>Helbert2019</cite>.
 == Kinetics and Mechanism ==

← Older revision		Revision as of 01:07, 13 June 2020
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	</biblio>		</biblio>

−	[[Category:~~Glycoside Hydrolase~~ Families\|PL030]]	+	[[Category:Polysaccharide Lyase Families\|PL030]]

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 <!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption -->
-{{UnderConstruction}}
+{{CuratorApproved}}
 * [[Author]]: [[User:Marie-Line Garron|Marie-Line Garron]]
 * [[Responsible Curator]]:  [[User:Marie-Line Garron|Marie-Line Garron]]

@@ Line 9: / Line 9: @@
 {| {{Prettytable}}
 |-
-|{{Hl2}} colspan="2" align="center" |'''Polysaccharide Lyase Family PL10'''
+|{{Hl2}} colspan="2" align="center" |'''Polysaccharide Lyase Family PL30'''
 |-
 |'''3D Structure'''