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Difference between revisions of "Carbohydrate-active enzymes"
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| − | + | * [[Responsible Curator]]: [[User:SpencerWilliams|Spencer Williams]] | |
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| − | + | Enzymatic formation and cleavage of the bond between two sugars or between a sugar and another group can occur by hydrolysis to give the free sugar (''glycosidases'' or ''glycoside hydrolases''), by transglycosylation to give a new glycoside (''transglycosidases''), by phosphorolysis to give the sugar-1-phosphate (''phosphorylases'') or by elimination to give unsaturated sugar products (''lyases''). The principal enzymes that catalyze glycoside synthesis are nucleotide phosphosugar-dependent ''glycosyltransferases''. | |
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| − | Enzymatic formation and cleavage of the bond between two sugars or between a sugar and another group can occur by hydrolysis to give the free sugar (glycosidases or glycoside hydrolases), by transglycosylation to give a new glycoside (transglycosidases), by phosphorolysis to give the sugar-1-phosphate (phosphorylases) or by elimination to give unsaturated sugar products (lyases). The principal enzymes that catalyze glycoside synthesis are nucleotide phosphosugar-dependent glycosyltransferases. | ||
== Substrate specificities == | == Substrate specificities == | ||
Revision as of 03:23, 9 July 2009
| Glycoside Hydrolase Family GHnn | |
| Clan | GH-x |
| Mechanism | retaining/inverting |
| Active site residues | known/not known |
| CAZy DB link | |
| http://www.cazy.org/fam/GHnn.html | |
Enzymatic formation and cleavage of the bond between two sugars or between a sugar and another group can occur by hydrolysis to give the free sugar (glycosidases or glycoside hydrolases), by transglycosylation to give a new glycoside (transglycosidases), by phosphorolysis to give the sugar-1-phosphate (phosphorylases) or by elimination to give unsaturated sugar products (lyases). The principal enzymes that catalyze glycoside synthesis are nucleotide phosphosugar-dependent glycosyltransferases.
Substrate specificities
Kinetics and Mechanism
Catalytic Residues
Three-dimensional structures
Family Firsts
- First sterochemistry determination
- Cite some reference here, with a short explanation [1].
- First catalytic nucleophile identification
- First general acid/base residue identification
- First 3-D structure
References
- Comfort DA, Bobrov KS, Ivanen DR, Shabalin KA, Harris JM, Kulminskaya AA, Brumer H, and Kelly RM. (2007). Biochemical analysis of Thermotoga maritima GH36 alpha-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D glycoside hydrolases. Biochemistry. 2007;46(11):3319-30. DOI:10.1021/bi061521n |
[[Category:Glycoside Hydrolase Families]]