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Difference between revisions of "Carbohydrate-active enzymes"

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* [[Responsible Curator]]:  [[User:SpencerWilliams|Spencer Williams]]
 
* [[Responsible Curator]]:  [[User:SpencerWilliams|Spencer Williams]]
 
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<div style="float:right">
 
{| {{Prettytable}}
 
|-
 
|{{Hl2}} colspan="2" align="center" |'''Glycoside Hydrolase Family GHnn'''
 
|-
 
|'''Clan'''   
 
|GH-x
 
|-
 
|'''Mechanism'''
 
|retaining/inverting
 
|-
 
|'''Active site residues'''
 
|known/not known
 
|-
 
|{{Hl2}} colspan="2" align="center" |'''CAZy DB link'''
 
|-
 
| colspan="2" |http://www.cazy.org/fam/GHnn.html
 
|}
 
</div>
 
  
 
Enzymatic formation and cleavage of the bond between two sugars or between a sugar and another group can occur by hydrolysis to give the free sugar (''glycosidases'' or ''glycoside hydrolases''), by transglycosylation to give a new glycoside (''transglycosidases''), by phosphorolysis to give the sugar-1-phosphate (''phosphorylases'') or by elimination to give unsaturated sugar products (''lyases''). The principal enzymes that catalyze glycoside synthesis are nucleotide phosphosugar-dependent ''glycosyltransferases''.
 
Enzymatic formation and cleavage of the bond between two sugars or between a sugar and another group can occur by hydrolysis to give the free sugar (''glycosidases'' or ''glycoside hydrolases''), by transglycosylation to give a new glycoside (''transglycosidases''), by phosphorolysis to give the sugar-1-phosphate (''phosphorylases'') or by elimination to give unsaturated sugar products (''lyases''). The principal enzymes that catalyze glycoside synthesis are nucleotide phosphosugar-dependent ''glycosyltransferases''.
== Substrate specificities ==
 
 
 
 
== Kinetics and Mechanism ==
 
 
 
 
== Catalytic Residues ==
 
 
 
 
== Three-dimensional structures ==
 
 
 
  
== Family Firsts ==
 
;First sterochemistry determination: Cite some reference here, with a ''short'' explanation <cite>1</cite>.
 
;First catalytic nucleophile identification:
 
;First general acid/base residue identification:
 
;First 3-D structure:
 
  
 
== References ==
 
== References ==

Revision as of 04:02, 9 July 2009


Enzymatic formation and cleavage of the bond between two sugars or between a sugar and another group can occur by hydrolysis to give the free sugar (glycosidases or glycoside hydrolases), by transglycosylation to give a new glycoside (transglycosidases), by phosphorolysis to give the sugar-1-phosphate (phosphorylases) or by elimination to give unsaturated sugar products (lyases). The principal enzymes that catalyze glycoside synthesis are nucleotide phosphosugar-dependent glycosyltransferases.


References

  1. Comfort DA, Bobrov KS, Ivanen DR, Shabalin KA, Harris JM, Kulminskaya AA, Brumer H, and Kelly RM. (2007). Biochemical analysis of Thermotoga maritima GH36 alpha-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D glycoside hydrolases. Biochemistry. 2007;46(11):3319-30. DOI:10.1021/bi061521n | PubMed ID:17323919 [1]

[[Category:Glycoside Hydrolase Families]]