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Difference between revisions of "Carbohydrate Binding Module Family 44"
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== Functionalities == | == Functionalities == | ||
| − | Carbohydrate binding and targeting were described as functions of CBM44. While carbohydrate binding was confirmed by AGE and ITC, the targeting effect was analyzed by the ability of CBM44 to maintain enzyme activity of [[GH44]] (Cel44A) exposed to a substrate mixture. The ensemble of a [[GH44]] and a CBM44 was found in the multimodular cellulase CtCel9D-Cel44A from ''Acetivibrio thermocellus'' and ligand spectra of both modules are consistent <cite>Najmudin2006</cite>. In the targeting experiment, the fused [[GH44]]-CBM44 construct was compared to the truncated [[GH44]]. In separate xyloglucan or carboxymethylcellulose incubations, the activity of GH44-CBM44 was comparable to that of [[GH44]] alone. In a next step, substrate mixtures were used. These mixtures also contained laminarin and pustulan, which are not degraded. While [[GH44]] activity now decreased, activity was restored by [[GH44]]-CBM44. Thus, giving the complex carbohydrate mixture in plant cell walls, CBM44 may guide the enzyme to its target sugars. Such effects have also been achieved in synthetic CBM44-enzyme chimeras, e.g., [[GH12]] or [[GH28]] <cite>#Furtado215 #Carli2022</cite>. | + | Carbohydrate binding and targeting were described as functions of CBM44. While carbohydrate binding was confirmed by AGE and ITC, the [[Carbohydrate-binding modules|targeting effect]] was analyzed by the ability of CBM44 to maintain enzyme activity of [[GH44]] (Cel44A) exposed to a substrate mixture. The ensemble of a [[GH44]] and a CBM44 was found in the multimodular cellulase CtCel9D-Cel44A from ''Acetivibrio thermocellus'' and ligand spectra of both modules are consistent <cite>Najmudin2006</cite>. In the targeting experiment, the fused [[GH44]]-CBM44 construct was compared to the truncated [[GH44]]. In separate xyloglucan or carboxymethylcellulose incubations, the activity of GH44-CBM44 was comparable to that of [[GH44]] alone. In a next step, substrate mixtures were used. These mixtures also contained laminarin and pustulan, which are not degraded. While [[GH44]] activity now decreased, activity was restored by [[GH44]]-CBM44. Thus, giving the complex carbohydrate mixture in plant cell walls, CBM44 may guide the enzyme to its target sugars. Such effects have also been achieved in synthetic CBM44-enzyme chimeras, e.g., [[GH12]] or [[GH28]] <cite>#Furtado215 #Carli2022</cite>. |
== Family Firsts == | == Family Firsts == | ||
Revision as of 02:40, 9 January 2023
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
| CAZy DB link | |
| http://www.cazy.org/CBM44.html |
Ligand specificities
CBM44 targets ß-1,4-polymers such as xyloglucan and cellulose (hydroxyethylcellulose and Avicel), mixed linkage ß-1,3/ß1,4- glucans (lichenan and barley) or glucomannan (konjac) [1]. Affinity for xylan was very low and binding to laminarin, curdlan, pullulan, pustulan, galactomannan or galactan was negative. Isothermal titration calorimetry (ITC) revealed highest affinity for xyloglucan as a polysaccharide, which was comparable to the affinity for cellohexaose as an oligosaccharide. For other cello-oligosaccharides, this affinity decreased with decreasing chain length, while no binding was detected for cellotriose.
Structural Features
Like many CBMs, CBM44 exhibits a typical ß-sandwich fold: two antiparallel ß-sheets form a concave and a convex surface. The concave surface forms a deep hydrophobic ligand-binding cleft that is estimated to accommodate up to five glucose residues (~24 Å) [1]. Here, three tryptophans (W189, W194 and W198) act as key residues to mediate ligand binding, as confirmed by affinity gel electrophoresis (AGE) and ITC analyses of specific mutants. The orientation of the tryptophans corresponds to the slightly twisted conformation of cello-oligosaccharides in solution.
Functionalities
Carbohydrate binding and targeting were described as functions of CBM44. While carbohydrate binding was confirmed by AGE and ITC, the targeting effect was analyzed by the ability of CBM44 to maintain enzyme activity of GH44 (Cel44A) exposed to a substrate mixture. The ensemble of a GH44 and a CBM44 was found in the multimodular cellulase CtCel9D-Cel44A from Acetivibrio thermocellus and ligand spectra of both modules are consistent [1]. In the targeting experiment, the fused GH44-CBM44 construct was compared to the truncated GH44. In separate xyloglucan or carboxymethylcellulose incubations, the activity of GH44-CBM44 was comparable to that of GH44 alone. In a next step, substrate mixtures were used. These mixtures also contained laminarin and pustulan, which are not degraded. While GH44 activity now decreased, activity was restored by GH44-CBM44. Thus, giving the complex carbohydrate mixture in plant cell walls, CBM44 may guide the enzyme to its target sugars. Such effects have also been achieved in synthetic CBM44-enzyme chimeras, e.g., GH12 or GH28 [2, 3].
Family Firsts
- First Identified
- Insert archetype here, possibly including very brief synopsis.
- First Structural Characterization
- Insert archetype here, possibly including very brief synopsis.
References
- Najmudin S, Guerreiro CI, Carvalho AL, Prates JA, Correia MA, Alves VD, Ferreira LM, Romão MJ, Gilbert HJ, Bolam DN, and Fontes CM. (2006). Xyloglucan is recognized by carbohydrate-binding modules that interact with beta-glucan chains. J Biol Chem. 2006;281(13):8815-28. DOI:10.1074/jbc.M510559200 |
- Furtado GP, Santos CR, Cordeiro RL, Ribeiro LF, de Moraes LA, Damásio AR, Polizeli Mde L, Lourenzoni MR, Murakami MT, and Ward RJ. (2015). Enhanced xyloglucan-specific endo-β-1,4-glucanase efficiency in an engineered CBM44-XegA chimera. Appl Microbiol Biotechnol. 2015;99(12):5095-107. DOI:10.1007/s00253-014-6324-0 |
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Carli S, Meleiro LP, Salgado JCS, Ward RJ. Synthetic carbohydrate-binding module-endogalacturonase chimeras increase catalytic efficiency and saccharification of lignocellulose residues (2022). Biomass Conv Bioref. 2022. DOI: 10.1007/s13399-022-02716-6.