View source for Carbohydrate Binding Module Family 5


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* [[Author]]: ^^^Manjeet Kaur^^^
* [[Responsible Curator]]:  ^^^Appa Rao Podile^^^
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== Ligand specificities ==
The family 5 carbohydrate binding modules (CBM5) are of approximately 60 residues. These occur as accessory domains in chitinases <cite>Kezuka2006 Van2000 Uni2012 Manjeet2013</cite>, endoglucanases <cite>Brun1997</cite> and lytic polysaccharide mono-oxygenases (LPMOs) <cite>Forsberg2016 Mutahir2018 Manjeet2019</cite>. This family first originated as a cellulose binding domain family V (CeBD V) <cite>Kezuka2006 Simpson1999</cite>. However, CBM5 have now been reported to have affinity to both cellulose and chitin <cite>Kezuka2006 Van2000 Uni2012 Manjeet2013 Brun1997 Forsberg2016 Mutahir2018 Manjeet2019 Simpson1999</cite>. Deletion of CBM5 from chitinases or LPMOs has shown considerable reduction in chitin binding <cite>Manjeet2013 Forsberg2016 Manjeet2019</cite>. The ''K<sub>d</sub>'' and ''B<sub>max</sub>'' values for CBM5 of an LPMO from ''Cellvibrio japonicas'', ''Cj''LPMO10A for α-chitin, were 5.3 µM and 4.8 µmol/g α-chitin, respectively <cite>Forsberg2016</cite>. The ''K<sub>d</sub>'' values of CBM5 from ''Bacillus thuringiensis'', ''Bt''CBM5 for α- and β-chitin were in the order of 0.6-0.7 µM, whereas the ''B<sub>max</sub>'' value was ~1.9 µmol/g for both α- and β-chitin <cite>Manjeet2019</cite>.

== Structural Features ==
The structures of CBM5 domains have been elucidated for an endoglucanase, CBDEGZ from ''Erwinia chrysanthemi'' (''Ec''EGZCBM5) and two chitinases ChBDChiB from ''Serratia marcescens'' (''Sm''ChiBCBM5) and ChBDChiC from ''Streptomyces griseus'' HUT6037 (''Sg''ChiCCBM5) <cite>Kezuka2006 Van2000 Brun1997</cite>.

The three structures revealed that CBM5 is composed of five β-strands (β1-5) <cite>Kezuka2006 Van2000 Brun1997</cite>. The β1, β2 and β3 forms the principle structure and the additional short β-strands (β4 and β5) form an antiparallel β-sheet which is independent of the main strand (Figure 1). The ''Ec''EGZCBM5 resembles a ski-boot or L-shaped structure composed of only β-sheets <cite>Brun1997</cite>. Helix structures have not been found in CBM5 modules.

There are a few differences in CBM5 modules of endo-glucanases (''Ec''EGZCBM5) and chitinases (''Sm''ChiBCBM5 and ''Sg''ChiCCBM5). The ''Ec''EGZCBM5 possesses a conserved disulfide bond between Cys4 and Cys61 <cite>Brun1997</cite>. These disulfide bonds have not been reported in ''Sm''ChiBCBM5 and ''Sg''ChiCCBM5 <cite>Kezuka2006 Van2000</cite>.

CBM5 modules possess surface exposed aromatic residues which interact with polysaccharides most probably through hydrophobic interactions <cite>Kezuka2006 Simpson1999</cite>. The ''Ec''EGZCBM5 possesses three exposed aromatic residues Trp18, Trp43 and Tyr44. Trp18 is present on an extra loop and is linearly aligned to Trp43 and Tyr44 and extends the substrate binding site <cite>Brun1997</cite>. The three residues are essential for complete binding of ''Ec''EGZCBM5. Polar residues like Asp17 are present on cellulose binding face and form H-bonds to stabilize the appropriate orientation of cellulose binding residues<cite>Brun1997</cite>. Polar residues also form H-bonds with oxygen atom and/or OH-groups of glucose subunits of cellulose, thus have been proposed to play a role in cellulose-disruption <cite>Brun1997</cite>. Mutation of Asp17 resulted in decreased binding towards cellulose <cite>Simpson1999</cite>.

''Sm''ChiBCBM5 and ''Sg''ChiCCBM5 have only two surface exposed aromatic residues, Trp479 and Trp481 in ''Sm''ChiBCBM5 and Trp59 and Trp60 in ''Sg''ChiCCBM5; whose structural homologues in ''Ec''EGZCBM5 are Trp43 and Tyr44 <cite>Van2000</cite>. The two exposed aromatic residues are sufficient for binding in ''Sm''ChiBCBM5 and ''Sg''ChiCCBM5 <cite>Kezuka2006</cite>. These residues interact extensively and play a vital role in increasing the proximity of substrate through hydrophobic interactions. It has been proposed that either of the two exposed residues should be a tryptophan residue <cite>Kezuka2006</cite>. The Tyr-Tyr pair has not been found in the family <cite>Kezuka2006</cite>.

In ''Sg''ChiCCBM5, six residues (Trp36, Val 48, Tyr 50, Tyr55, Pro66 and Trp72) participate in forming a hydrophobic core in the domain centre. The side chain of Pro66 is internally buried while the remaining 5 residues form the hydrophobic socket <cite>Kezuka2006</cite>. Only two surface exposed aromatic residues (Trp59 and Trp60) are involved in carbohydrate binding which are positioned on a loop between the sheets β2 and β5 <cite>Kezuka2006</cite>. When protein-substrate interactions were studied between ''Sg''ChiCCBM5 and tri-N-acetyl-chitotriose, it was found that the ligand binding was facilitated by two stacking interactions (Trp59-NAG-1 and Trp-NAG3) and two H-bonds (Trp60-N and NAG2-O7 and Trp56-NE1 and NAG2-O6) <cite>Kezuka2006</cite>.

== Functionalities == 
Multi-modular enzymes like endo-glucanases, chitinases and LPMOs possess CBM5 modules as accessory domains appended to their catalytic domain, either directly or with the help of linkers like FnIII domains <cite>Kezuka2006 Van2000 Uni2012 Manjeet2013 Brun1997 Forsberg2016 Mutahir2018 Manjeet2019</cite>. The CBM5 domains are responsible for increased affinity of these enzymes towards crystalline cellulose or chitin. Their presence also increases the efficiency of enzymes to bind to substrates in a broader pH range <cite>Uni2012 Manjeet2019</cite>. Deletion of CBM5 domain resulted in reduction or complete loss of binding in several instances <cite>Manjeet2013</cite>. Deletion of C-terminal FnIII and CBM5 domains from BliChi resulted in 5-fold reduction of hydrolytic activity on β-chitin. Also the mutant was unable to degrade α-chitin <cite>Manjeet2013</cite>. Accessory domains have thus been suggested to play an important role in hydrolysis by moving the enzymes in close proximity of substrates <cite>Manjeet2019</cite>. Presence of CBM5 domains in LPMOs have been shown to alter the product profile while acting on crystalline β-chitin substrates <cite>Manjeet2019</cite>. In ''Bc''LPMO10A, CBM5 promoted substrate binding as well as protected the enzyme from inactivation <cite>Mutahir2018</cite>.

== Family Firsts ==
;First indentified: CBM5 modules were first discovered in Endoglucanase, CBDEGZ from ''Erwinia chrysanthemi'' <cite>Brun1997</cite>.
;First structural characterization: The first NMR derived structure of CBM5 was from CBDEGZ <cite>Brun1997</cite> and first crystal structure was studied for ChBDChiB from ''Serratia marcescens'' (''Sm''ChiBCBM5) <cite>Van2000</cite>.

== References ==
<biblio>
#Kezuka2006 pmid=16516924
# Van2000 pmid=10823940
Van Aalten     D.M.F., Synstad B., Brurberg M.B., Hough E., Riise B.W., Eijsink V.G.H.,     Wierenga R.K. (2000) Structure of a two-domain chitotriosidase from ''Serratia marcescens'' at 1.9 Å     resolution.  PNAS, 97(11),     5842-5847. DOI:10.1073/pnas.97.11.5842
#Uni2012 pmid=22451396
Uni F., Lee S., Yatsunami R., Fukui T.,     Nakamura S. (2012) Mutational analysis of a CBM5 family 5 Chitin binding     domain of an alkaline chitinase from ''Bacillus''     sp. J813. Biosci. Biotechnol. Biochem. 76(3), 530-535. DOI:     10.1271/bbb.110835
#Manjeet2013 pmid=23480960
Manjeet K.,     Purushotham P., Neeraja C., Podile A.R. (2013) Bacterial chitin binding     proteins show differential binding and synergy with chitinases. Microbiol     Res. 168(7), 461-468. DIO:     10.1016/j.micres.2013.01.006
#Brun1997 pmid=9405041
Brun E.,     Moriaud F., Gans P., Blackledge M.J., Barras F., Marion D. (1997) Solution     structure of the cellulose binding domain of the endoglucanase Z secreted     be ''Erwinia chrysanthemi''.     Biochemistry 36, 16074-16086. DOI: 10.1021/bi9718494
#Forsberg2016 pmid=26858252
Forsberg Z.,     Nelson C.E., Dalhus B., Mekasha S., Loose J.S.M., Crouch L.I., Rohr A.K.,     Gardner J.G., Eijsink V.G.H., Vaaje Kolstad G. (2016) Structural and     functional analysis of a lytic polysaccharide monooxygenase important for     efficient utilization of chitin in ''Cellvibrio     japonicus''. J. Biol Chem. 291(14), 7300-7312. DOI: 10.1074/jbc.M115.700161
#Mutahir2018 pmid=29993123
Mutahir Z.,     Mekasha S., Loose J.S.M., Abbas F., Vaaje-Kolstad G., Eijsink V.G.H.,     Forsberg Z. (2018) Characterization and synergistic action of a     tetra-modular lytic polysaccharide monooxygenase from ''Bacillus cereus''. FEBS Lett. 592(15), 2562-2571. DOI:10.1002/1873-3468.13189
#Manjeet2019 pmid=30708015
Manjeet K.,     Madhuprakash J., Mormann M., Moerschbacher B.M., Podile A.R. (2019) A     carbohydrate binding module 5 is essential for oxidative cleavage of     chitin by a multi-modular lytic polysaccharide monooxygenase from ''Bacillus thuringiensis'' serovar ''kurstaki''. Int J Biol Macromol. 127,     649-656. DOI: 10.1016/j.ijbiomac.2019.01.183
#Simpson1999 pmid=10419961
Simpson H.D. and Barras F. (1999) Functional analysis of the carbohydrate binding     domains of ''Erwinia chrysanthemi''     Cel5 (endoglucanase Z) and an ''Escherichia     coli'' putative chitinase. J Bacteriol. 181 (15), 4611-4616.
</biblio>

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