New to the CAZy classification? Read this first.
Want to learn more about CAZypedia? Read the CAZypedia 10th anniversary article in Glycobiology.

Difference between revisions of "Carbohydrate Binding Module Family 82"

From CAZypedia
Jump to: navigation, search
Line 18: Line 18:
  
 
== Ligand specificities ==
 
== Ligand specificities ==
Mention here all major natural ligand specificities that are found within a given family (also plant or mammalian origin). Certain linkages and promiscuity would also be mentioned here if biologically relevant.
+
Only one member of this family has been characterized to date, the first CBM found in the cell-wall anchored Amy13K from Eubacterium rectale. It was found to bind beta-cyclodextrin and glycogen with similar affinity, with slightly weaker affinity for maltoheptaose as determined by isothermal titration calorimetry. The module was also found to bind to corn starch granules, both from a wild-type source and from a high amylose source (HiMaize 260) with approximately equal affinity but did not demonstrate binding to potato starch or a chemically crosslinked starch (Fibersym) as determined via depletion assays. Binding to amylopectin and pullulan was also demonstrated via affinity electrophoresis.
 
+
<cite>Cockburn2018</cite>.
''Note: Here is an example of how to insert references in the text, together with the "biblio" section below:'' Please see these references for an essential introduction to the CAZy classification system: <cite>DaviesSinnott2008 Cantarel2009</cite>. CBMs, in particular, have been extensively reviewed <cite>Boraston2004 Hashimoto2006 Shoseyov2006 Guillen2010 Armenta2017</cite>.
 
  
 
== Structural Features ==
 
== Structural Features ==
''Content in this section should include, in paragraph form, a description of:''
+
There is currently no structures solved for this family, however, secondary structure analysis and alignments suggest they are likely to be beta-sandwich type folds similar to the [CBM41] family.  
* '''Fold:''' Structural fold (beta trefoil, beta sandwich, etc.)
 
* '''Type:''' Include here Type A, B, or C and properties
 
* '''Features of ligand binding:''' Describe CBM binding pocket location (Side or apex) important residues for binding (W, Y, F, subsites), interact with reducing end, non-reducing end, planar surface or within polysaccharide chains. Include examples pdb codes. Metal ion dependent. Etc.
 
  
 
== Functionalities ==  
 
== Functionalities ==  
''Content in this section should include, in paragraph form, a description of:''
+
This family has been found to be exclusively associated with [GH13] family amylases from a quite narrow taxonomic range within Roseburia and Eubacterium rectale. Removal of the only CBM82 from the E. rectale Amy13K enzyme resulted in an approximately 2-fold decrease in activity of the enzyme towards amylopectin or potato starch, but resulted in a larger 5-fold decrease in the activity of the enzyme towards corn starch granules, suggesting an important role in targeting the enzyme to this substrate. <cite>Cockburn2018</cite>  
* '''Functional role of CBM:''' Describe common functional roles such as targeting, disruptive, anchoring, proximity/position on substrate.
 
* '''Most Common Associated Modules:''' 1. Glycoside Hydrolase Activity; 2. Additional Associated Modules (other CBM, FNIII, cohesin, dockerins, expansins, etc.)
 
* '''Novel Applications:''' Include here if CBM has been used to modify another enzyme, or if a CBM was used to label plant/mammalian tissues? Etc.
 
  
 
== Family Firsts ==
 
== Family Firsts ==
;First Identified
+
;First Identified in E. rectale Amy13K, establishing the family <cite>Cockburn2018</cite>
:Insert archetype here, possibly including ''very brief'' synopsis.
+
 
;First Structural Characterization
 
:Insert archetype here, possibly including ''very brief'' synopsis.
 
  
 
== References ==
 
== References ==
 
<biblio>
 
<biblio>
#Cantarel2009 pmid=18838391
+
#Cockburn2018 pmid=29139580
#DaviesSinnott2008 Davies, G.J. and Sinnott, M.L. (2008) Sorting the diverse: the sequence-based classifications of carbohydrate-active enzymes. ''The Biochemist'', vol. 30, no. 4., pp. 26-32. [http://www.biochemist.org/bio/03004/0026/030040026.pdf Download PDF version].
 
#Boraston2004 pmid=15214846
 
#Hashimoto2006 pmid=17131061
 
#Shoseyov2006 pmid=16760304
 
#Guillen2010 pmid=19908036
 
#Armenta2017 pmid=28547780
 
 
</biblio>
 
</biblio>
  
 
[[Category:Carbohydrate Binding Module Families|CBM082]] <!-- ATTENTION: Make sure to replace "nnn" with a three digit family number, e.g. "032" or "105" etc., for proper sorting of the page by family number. -->
 
[[Category:Carbohydrate Binding Module Families|CBM082]] <!-- ATTENTION: Make sure to replace "nnn" with a three digit family number, e.g. "032" or "105" etc., for proper sorting of the page by family number. -->

Revision as of 11:48, 8 May 2019

Under construction icon-blue-48px.png
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.

CAZy DB link
http://www.cazy.org/CBM82.html

Ligand specificities

Only one member of this family has been characterized to date, the first CBM found in the cell-wall anchored Amy13K from Eubacterium rectale. It was found to bind beta-cyclodextrin and glycogen with similar affinity, with slightly weaker affinity for maltoheptaose as determined by isothermal titration calorimetry. The module was also found to bind to corn starch granules, both from a wild-type source and from a high amylose source (HiMaize 260) with approximately equal affinity but did not demonstrate binding to potato starch or a chemically crosslinked starch (Fibersym) as determined via depletion assays. Binding to amylopectin and pullulan was also demonstrated via affinity electrophoresis. [1].

Structural Features

There is currently no structures solved for this family, however, secondary structure analysis and alignments suggest they are likely to be beta-sandwich type folds similar to the [CBM41] family.

Functionalities

This family has been found to be exclusively associated with [GH13] family amylases from a quite narrow taxonomic range within Roseburia and Eubacterium rectale. Removal of the only CBM82 from the E. rectale Amy13K enzyme resulted in an approximately 2-fold decrease in activity of the enzyme towards amylopectin or potato starch, but resulted in a larger 5-fold decrease in the activity of the enzyme towards corn starch granules, suggesting an important role in targeting the enzyme to this substrate. [1]

Family Firsts

First Identified in E. rectale Amy13K, establishing the family [1]


References

  1. Cockburn DW, Suh C, Medina KP, Duvall RM, Wawrzak Z, Henrissat B, and Koropatkin NM. (2018) Novel carbohydrate binding modules in the surface anchored α-amylase of Eubacterium rectale provide a molecular rationale for the range of starches used by this organism in the human gut. Mol Microbiol. 107, 249-264. DOI:10.1111/mmi.13881 | PubMed ID:29139580 | HubMed [Cockburn2018]