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Carbohydrate Esterase Family 2

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Carbohydrate Esterase Family CE2
Clan
Mechanism
Active site residues
CAZy DB link
http://www.cazy.org/CE3.html

Substrate specificities

All of the well characterized carbohydrate esterase family 2 enzymes have been shown to remove acetate groups from the synthetic molecule, 4-nitrophenyl acetate (4p-NP-AcC) (Montanier et al. 2009; Till et al. 2013). Contrary to typical acetyl xylan esterases, CE2 family members are shown to have a strong preference for the deacetylation of xylopyranosides at positions 3 and 4 instead of the typical deacetylation at position 2. CE2 family members were also shown to have significant preference for deacetylation of glucopyranosyl and mannopyranosyl residues at the 6-O position and significantly preferred deacetylation of glucopyranosyl and mannopyranosyl residues relative to the deacetylation of xylopyranosides. For these reasons CE2 family members are considered to be 6-de-O-acetylases (Topakas et al. 2010).

Catalytic Residues

Kinetics and Mechanism

Three-dimensional structures

Family Firsts

First characterized
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First mechanistic insight
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First 3-D structure
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References

  1. Faik A (2010). Xylan biosynthesis: news from the grass. Plant Physiol. 2010;153(2):396-402. DOI:10.1104/pp.110.154237 | PubMed ID:20375115 [Faik2010]