Glycoside Hydrolase Family 124

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• Author: ^^^Harry Gilbert^^^
• Responsible Curator: ^^^Harry Gilbert^^^

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Glycoside Hydrolase Family GH124
Clan	None
Mechanism	Inverting
Active site residues	Catalytic acid known
CAZy DB link
http://www.cazy.org/GH124.html

Substrate specificities

Family GH124 consists of a small number of cellulosomal proteins. The Clostridium thermocellum enzyme CtCel124A is the only member of this family that has been characterized. The enzyme is an endo-β1,4-glucanase with modest activity in vitro, but acts in synergy with the major exo-cellulase from C. thermocellum and, as a discrete entity, is able to deconstruct tobacco cell walls [1].

Kinetics and Mechanism

Using cellopentaose as the substrate and HPLC the enzyme was shown to display a single displacement (inverting) mechanism [1].

Catalytic Residues

The catalytic acid in CtCel124A was shown to be Glu96 based on the crystal structural of the enzyme and the observation that the E96A mutation completely inactivates the cellulase [1]. The enzyme contains no candidate catalytic base and it was suggested that the nucleophilic water was activated by a "Grotthus-like" mechanism, similar to some GH6 cellulases [2].

Three-dimensional structures

The enzyme displays a superhelical fold in which a constellation of α-helices encircle a central helix that houses the catalytic apparatus. The catalytic acid, Glu96, is located at the C-terminus of the central helix. The substrate-binding cleft can be divided into two discrete topographical domains in which the bound cellotriose molecules display twisted and linear conformations, respectively, suggesting that the enzyme may target the interface between crystalline and disordered regions of cellulose. The active site of CtCel124 displays remarkable structural conservation with a GH23 lytic transglycosylase.

Family Firsts

First stereochemistry determination

The sterochemical outcome of catalysis is inversion of the β linkage into the α anomer [1]. The method used to detect the stereochemical outcome was the HPLC method of Braun et al. [3]. The reaction was too slow to detect through NMR as mutarotation obscured the sterochemistry of glycosidic bond cleavage.

First catalytic nucleophile identification

The candidate catalytic base has been identified.

First general acid/base residue identification

The catalytic acid was identified from structure in harness with mutagenesis and sequence conservation [1].

First 3-D structure

The crystal structure of CtCel124 was determined in complex with two cellotriose molecules. The structure revealed a novel cellulase fold and hinted that the enzyme attacks cellulose at the interface between two structural domains of the substrate [1].

References

1. Brás JL, Cartmell A, Carvalho AL, Verzé G, Bayer EA, Vazana Y, Correia MA, Prates JA, Ratnaparkhe S, Boraston AB, Romão MJ, Fontes CM, and Gilbert HJ. (2011). Structural insights into a unique cellulase fold and mechanism of cellulose hydrolysis. Proc Natl Acad Sci U S A. 2011;108(13):5237-42. DOI:10.1073/pnas.1015006108 |
2. Koivula A, Ruohonen L, Wohlfahrt G, Reinikainen T, Teeri TT, Piens K, Claeyssens M, Weber M, Vasella A, Becker D, Sinnott ML, Zou JY, Kleywegt GJ, Szardenings M, Ståhlberg J, and Jones TA. (2002). The active site of cellobiohydrolase Cel6A from Trichoderma reesei: the roles of aspartic acids D221 and D175. J Am Chem Soc. 2002;124(34):10015-24. DOI:10.1021/ja012659q |
3. Braun C, Meinke A, Ziser L, and Withers SG. (1993). Simultaneous high-performance liquid chromatographic determination of both the cleavage pattern and the stereochemical outcome of the hydrolysis reactions catalyzed by various glycosidases. Anal Biochem. 1993;212(1):259-62. DOI:10.1006/abio.1993.1320 |