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Difference between revisions of "Glycoside Hydrolase Family 128"

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Family GH128 contains b-1,3-glucanases that cleave b-1,3 linkages in various b-glucans such as lentinan from Lentinula edodes, laminarin from Laminaria digitata, pachyman from Poria cocos and curdlan from Alcaligenes faecalis. The first GH128 enzyme, GLU1, was cloned from L. edodes fruiting bodies (shiitake mushroom). GLU1 did not degrade b-1,3-linkages within b-1,3-1,4-glucans such as barley glucan, indicating the enzyme is categorized into EC 3.2.1.39    <cite>Sakamoto2011</cite>.
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Family GH128 contains &beta;-1,3-glucanases that cleave b-1,3 linkages in various b-glucans such as lentinan from Lentinula edodes, laminarin from Laminaria digitata, pachyman from Poria cocos and curdlan from Alcaligenes faecalis. The first GH128 enzyme, GLU1, was cloned from L. edodes fruiting bodies (shiitake mushroom). GLU1 did not degrade b-1,3-linkages within b-1,3-1,4-glucans such as barley glucan, indicating the enzyme is categorized into EC 3.2.1.39    <cite>Sakamoto2011</cite>.
  
 
== Kinetics and Mechanism ==
 
== Kinetics and Mechanism ==

Revision as of 19:01, 21 July 2015

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Glycoside Hydrolase Family GH128
Clan GH-x
Mechanism retaining/inverting
Active site residues known/not known
CAZy DB link
http://www.cazy.org/GH128.html


Substrate specificities

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Family GH128 contains β-1,3-glucanases that cleave b-1,3 linkages in various b-glucans such as lentinan from Lentinula edodes, laminarin from Laminaria digitata, pachyman from Poria cocos and curdlan from Alcaligenes faecalis. The first GH128 enzyme, GLU1, was cloned from L. edodes fruiting bodies (shiitake mushroom). GLU1 did not degrade b-1,3-linkages within b-1,3-1,4-glucans such as barley glucan, indicating the enzyme is categorized into EC 3.2.1.39 [1].

Kinetics and Mechanism

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Catalytic Residues

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Three-dimensional structures

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Family Firsts

First stereochemistry determination
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First catalytic nucleophile identification
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First general acid/base residue identification
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First 3-D structure
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References

  1. Sakamoto Y, Nakade K, and Konno N. (2011) Endo-β-1,3-glucanase GLU1, from the fruiting body of Lentinula edodes, belongs to a new glycoside hydrolase family. Appl Environ Microbiol. 77, 8350-4. DOI:10.1128/AEM.05581-11 | PubMed ID:21965406 | HubMed [Sakamoto2011]