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Difference between revisions of "Glycoside Hydrolase Family 31"
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== Substrate specificities == | == Substrate specificities == | ||
| − | + | CAZy Family GH31 is one of the two major families, along with GH13, that contain α-glucosidases. These enzymes play important roles in primary metabolism (e.g. human sucrase/isomaltase, a target for diabetic drugs such as miglitol), in catabolism (e.g. human lysosomal α-glucosidase) and in glycoprotein processing (e.g. ER glucosidase II). In addition to α-glucosidases, GH31 also contains α-xylosidases, isomaltosyltransferases, maltase/glucoamylases and the mechanistically interesting, non-hydrolytic [[Alpha-glucan lyases|α-glucan lyases]]. These enzymes can be found in a wide range of organisms including archaea, bacteria, plants and animals. Interestingly the two mammalian digestive enzymes are both duplicated genes, each with dual specificities. | |
== Kinetics and Mechanism == | == Kinetics and Mechanism == | ||
Revision as of 15:03, 13 August 2009
- Author: Ran Zhang
- Responsible Curator: Steve Withers
| Glycoside Hydrolase Family GH31 | |
| Clan | GH-D |
| Mechanism | retaining |
| Active site residues | known |
| CAZy DB link | |
| http://www.cazy.org/fam/GH31.html | |
Substrate specificities
CAZy Family GH31 is one of the two major families, along with GH13, that contain α-glucosidases. These enzymes play important roles in primary metabolism (e.g. human sucrase/isomaltase, a target for diabetic drugs such as miglitol), in catabolism (e.g. human lysosomal α-glucosidase) and in glycoprotein processing (e.g. ER glucosidase II). In addition to α-glucosidases, GH31 also contains α-xylosidases, isomaltosyltransferases, maltase/glucoamylases and the mechanistically interesting, non-hydrolytic α-glucan lyases. These enzymes can be found in a wide range of organisms including archaea, bacteria, plants and animals. Interestingly the two mammalian digestive enzymes are both duplicated genes, each with dual specificities.
Kinetics and Mechanism
Catalytic Residues
Three-dimensional structures
Family Firsts
- First sterochemistry determination
- Cite some reference here, with a short explanation [1].
- First catalytic nucleophile identification
- First general acid/base residue identification
- First 3-D structure