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Difference between revisions of "Glycoside Hydrolase Family 50"
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== Substrate specificities == | == Substrate specificities == | ||
| − | To date, all characterized [[glycoside hydrolases]] of family 50 are β-agarases (EC [{{EClink}}3.2.1.81 3.2.1.81]) that cleave β-1,4 glycosidic bonds of agarose, releasing neoagaro-biose | + | To date, all characterized [[glycoside hydrolases]] of family 50 are β-agarases (EC [{{EClink}}3.2.1.81 3.2.1.81]) that cleave β-1,4 glycosidic bonds of agarose, releasing neoagaro-biose and -tetraose <cite>REF1</cite>,<cite>REF2</cite>. Three enzymes, Aga50A and Aga50D from ''Saccharophagus degradans'' and Aga50B from ''Vibrio sp.'' have been reported to be pure exo-β-agarases <cite>REF3</cite>. |
| Line 52: | Line 52: | ||
== References == | == References == | ||
<biblio> | <biblio> | ||
| − | #REF1 pmid=8193156 | + | #REF1 pmid=8517750 |
| + | #REF2 pmid=8193156 | ||
| + | #REF3 pmid=19802606 | ||
</biblio> | </biblio> | ||
Revision as of 13:38, 29 May 2010
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
- Author:
- Responsible Curator: ^^^Mirjam Czjzek^^^
| Glycoside Hydrolase Family GH50 | |
| Clan | GH-A |
| Mechanism | probably retaining |
| Active site residues | inferred from clan GH-A as two Glu |
| CAZy DB link | |
| http://www.cazy.org/GH50.html | |
Substrate specificities
To date, all characterized glycoside hydrolases of family 50 are β-agarases (EC 3.2.1.81) that cleave β-1,4 glycosidic bonds of agarose, releasing neoagaro-biose and -tetraose [1],[2]. Three enzymes, Aga50A and Aga50D from Saccharophagus degradans and Aga50B from Vibrio sp. have been reported to be pure exo-β-agarases [3].
Kinetics and Mechanism
Content is to be added here.
Catalytic Residues
Content is to be added here.
Three-dimensional structures
Content is to be added here.
Family Firsts
- First stereochemistry determination
- Cite some reference here, with a short (1-2 sentence) explanation .
- First catalytic nucleophile identification
- Cite some reference here, with a short (1-2 sentence) explanation.
- First general acid/base residue identification
- Cite some reference here, with a short (1-2 sentence) explanation.
- First 3-D structure
- Cite some reference here, with a short (1-2 sentence) explanation.
References
- Sugano Y, Terada I, Arita M, Noma M, and Matsumoto T. (1993). Purification and characterization of a new agarase from a marine bacterium, Vibrio sp. strain JT0107. Appl Environ Microbiol. 1993;59(5):1549-54. DOI:10.1128/aem.59.5.1549-1554.1993 |
- Sugano Y, Matsumoto T, and Noma M. (1994). Sequence analysis of the agaB gene encoding a new beta-agarase from Vibrio sp. strain JT0107. Biochim Biophys Acta. 1994;1218(1):105-8. DOI:10.1016/0167-4781(94)90109-0 |
- Kim HT, Lee S, Lee D, Kim HS, Bang WG, Kim KH, and Choi IG. (2010). Overexpression and molecular characterization of Aga50D from Saccharophagus degradans 2-40: an exo-type beta-agarase producing neoagarobiose. Appl Microbiol Biotechnol. 2010;86(1):227-34. DOI:10.1007/s00253-009-2256-5 |