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Glycoside Hydrolase Family 74 - Revision history
2024-03-28T12:56:25Z
Revision history for this page on the wiki
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Harry Brumer: Text replacement - "\^\^\^(.*)\^\^\^" to "$1"
2021-12-18T21:19:20Z
<p>Text replacement - "\^\^\^(.*)\^\^\^" to "<a href="/index.php?title=User:$1&action=edit&redlink=1" class="new" title="User:$1 (page does not exist)">$1</a>"</p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 21:19, 18 December 2021</td>
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<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>* [[Author]]: <del class="diffchange diffchange-inline">^^^</del>Katsuro Yaoi<del class="diffchange diffchange-inline">^^^ </del>and <del class="diffchange diffchange-inline">^^^</del>Takuya Ishida<del class="diffchange diffchange-inline">^^^</del></div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>* [[Author]]: <ins class="diffchange diffchange-inline">[[User:Katsuro Yaoi|</ins>Katsuro Yaoi<ins class="diffchange diffchange-inline">]] </ins>and <ins class="diffchange diffchange-inline">[[User:</ins>Takuya Ishida<ins class="diffchange diffchange-inline">|Takuya Ishida]]</ins></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>* [[Responsible Curator]]: <del class="diffchange diffchange-inline">^^^</del>Katsuro Yaoi<del class="diffchange diffchange-inline">^^^</del></div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>* [[Responsible Curator]]: <ins class="diffchange diffchange-inline">[[User:</ins>Katsuro Yaoi<ins class="diffchange diffchange-inline">|Katsuro Yaoi]]</ins></div></td></tr>
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Harry Brumer
https://www.cazypedia.org/index.php?title=Glycoside_Hydrolase_Family_74&diff=14291&oldid=prev
Harry Brumer at 22:35, 25 September 2019
2019-09-25T22:35:41Z
<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 22:35, 25 September 2019</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l29" >Line 29:</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Substrate specificities ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Substrate specificities ==</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[Glycoside hydrolases]] of this family hydrolyze &beta;-1,4-linkages of various glucans. With the exception of Cel74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2336 ''Thermotoga maritima''], all biochemically characterized enzymes are specific toward xyloglucans and/or xyloglucan-oligosaccharides. Cel74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2336 ''Thermotoga maritima''] exhibits the highest activity on barley &beta;-glucan, with relative activity of 20% toward xyloglucan <cite>Chhabra2002</cite>. A wide diversity in the modes of action by GH74 enzymes has been reported. "Oligoxyloglucan reducing end-specific cellobiohydrolase (OXG-RCBH, EC [{{EClink}}3.2.1.150 3.2.1.150])" from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2002</cite> and "oligoxyloglucan reducing end-specific xyloglucanobiohydrolase (OREX)" from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=162425 ''Emericella nidulans''] (formerly known as ''Aspergillus nidulans'') <cite>Bauer2005</cite> are active on only xyloglucan oligosaccharides and have essentially no ability to degrade xyloglucan polysaccharides. They release oligosaccharides with two glucose units from non-reducing end of xyloglucan oligosaccharides. On the other hand, GH74 enzymes designated as xyloglucanase; xyloglucan specific endo-&beta;-1,4-glucanases: XEG; and xyloglucan hydrolases: Xgh, (EC [{{EClink}}3.2.1.151 3.2.1.151]), exhibit endo-type activity on xyloglucan from tamarind seed, a readily available and well-investigated xyloglucan <cite>York1993</cite>. Many GH74 xyloglucanases hydrolyze the glycosidic linkage of unbranched glucose residues, but several members including ''Geotrichum sp.'' OXG-RCBH <cite>Yaoi2002</cite>, ''E. nidulans'' OREX <cite>Bauer2005</cite>, and [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=51453''Hypocrea jecorina''] (formerly known as ''Trichoderma reesei'') Cel74A <cite>Desmet2007</cite> accommodate side-chain xylose residues at [[Sub-site nomenclature|subsite]] -1 of the active site. As first elucidated by Matsuzawa, Saito, and Yaoi <cite>Matsuzawa2014</cite>, some GH74 members exhibit processivity (multiple catalytic events before chain dissociation) in xyloglucan hydrolysis, which is related to active-site length and the presence of key aromatic residues, and which follows protein phylogeny <cite>Arnal2019</cite>.</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[Glycoside hydrolases]] of this family hydrolyze &beta;-1,4-linkages of various glucans. With the exception of Cel74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2336 ''Thermotoga maritima''], all biochemically characterized enzymes are specific toward xyloglucans and/or xyloglucan-oligosaccharides <ins class="diffchange diffchange-inline">(reviewed in <cite>Arnal2019</cite>)</ins>. Cel74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2336 ''Thermotoga maritima''] exhibits the highest activity on barley &beta;-glucan, with relative activity of 20% toward xyloglucan <cite>Chhabra2002</cite>. A wide diversity in the modes of action by GH74 enzymes has been reported. "Oligoxyloglucan reducing end-specific cellobiohydrolase (OXG-RCBH, EC [{{EClink}}3.2.1.150 3.2.1.150])" from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2002</cite> and "oligoxyloglucan reducing end-specific xyloglucanobiohydrolase (OREX)" from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=162425 ''Emericella nidulans''] (formerly known as ''Aspergillus nidulans'') <cite>Bauer2005</cite> are active on only xyloglucan oligosaccharides and have essentially no ability to degrade xyloglucan polysaccharides. They release oligosaccharides with two glucose units from non-reducing end of xyloglucan oligosaccharides. On the other hand, GH74 enzymes designated as xyloglucanase; xyloglucan specific endo-&beta;-1,4-glucanases: XEG; and xyloglucan hydrolases: Xgh, (EC [{{EClink}}3.2.1.151 3.2.1.151]), exhibit endo-type activity on xyloglucan from tamarind seed, a readily available and well-investigated xyloglucan <cite>York1993</cite>. Many GH74 xyloglucanases hydrolyze the glycosidic linkage of unbranched glucose residues, but several members including ''Geotrichum sp.'' OXG-RCBH <cite>Yaoi2002</cite>, ''E. nidulans'' OREX <cite>Bauer2005</cite>, and [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=51453''Hypocrea jecorina''] (formerly known as ''Trichoderma reesei'') Cel74A <cite>Desmet2007</cite> accommodate side-chain xylose residues at [[Sub-site nomenclature|subsite]] -1 of the active site. As first elucidated by Matsuzawa, Saito, and Yaoi <cite>Matsuzawa2014</cite>, some GH74 members exhibit processivity (multiple catalytic events before chain dissociation) in xyloglucan hydrolysis, which is related to active-site length and the presence of key aromatic residues, and which follows protein phylogeny <cite>Arnal2019</cite>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Kinetics and Mechanism ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Kinetics and Mechanism ==</div></td></tr>
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Harry Brumer
https://www.cazypedia.org/index.php?title=Glycoside_Hydrolase_Family_74&diff=14290&oldid=prev
Harry Brumer at 22:29, 25 September 2019
2019-09-25T22:29:09Z
<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 22:29, 25 September 2019</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l29" >Line 29:</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Substrate specificities ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Substrate specificities ==</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[Glycoside hydrolases]] of this family hydrolyze &beta;-1,4-linkages of various glucans. With the exception of Cel74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2336 ''Thermotoga maritima''], all biochemically characterized enzymes are specific toward xyloglucans and/or xyloglucan-oligosaccharides. Cel74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2336 ''Thermotoga maritima''] exhibits the highest activity on barley &beta;-glucan, with relative activity of 20% toward xyloglucan <cite>Chhabra2002</cite>. A wide diversity in the modes of action by GH74 enzymes has been reported. "Oligoxyloglucan reducing end-specific cellobiohydrolase (OXG-RCBH, EC [{{EClink}}3.2.1.150 3.2.1.150])" from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2002</cite> and "oligoxyloglucan reducing end-specific xyloglucanobiohydrolase (OREX)" from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=162425 ''Emericella nidulans''] (formerly known as ''Aspergillus nidulans'') <cite>Bauer2005</cite> are active on only xyloglucan oligosaccharides and have essentially no ability to degrade xyloglucan polysaccharides. They release oligosaccharides with two glucose units from non-reducing end of xyloglucan oligosaccharides. On the other hand, GH74 enzymes designated as xyloglucanase; xyloglucan specific endo-&beta;-1,4-glucanases: XEG; and xyloglucan hydrolases: Xgh, (EC [{{EClink}}3.2.1.151 3.2.1.151]), exhibit endo-type activity on xyloglucan from tamarind seed, a readily available and well-investigated xyloglucan <cite>York1993</cite>. Many GH74 xyloglucanases hydrolyze the glycosidic linkage of unbranched glucose residues, but several members including ''Geotrichum sp.'' OXG-RCBH <cite>Yaoi2002</cite>, ''E. nidulans'' OREX <cite>Bauer2005</cite>, and [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=51453''Hypocrea jecorina''] (formerly known as ''Trichoderma reesei'') Cel74A <cite>Desmet2007</cite> accommodate side-chain xylose residues at [[Sub-site nomenclature|subsite]] -1 of the active site.</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[Glycoside hydrolases]] of this family hydrolyze &beta;-1,4-linkages of various glucans. With the exception of Cel74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2336 ''Thermotoga maritima''], all biochemically characterized enzymes are specific toward xyloglucans and/or xyloglucan-oligosaccharides. Cel74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2336 ''Thermotoga maritima''] exhibits the highest activity on barley &beta;-glucan, with relative activity of 20% toward xyloglucan <cite>Chhabra2002</cite>. A wide diversity in the modes of action by GH74 enzymes has been reported. "Oligoxyloglucan reducing end-specific cellobiohydrolase (OXG-RCBH, EC [{{EClink}}3.2.1.150 3.2.1.150])" from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2002</cite> and "oligoxyloglucan reducing end-specific xyloglucanobiohydrolase (OREX)" from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=162425 ''Emericella nidulans''] (formerly known as ''Aspergillus nidulans'') <cite>Bauer2005</cite> are active on only xyloglucan oligosaccharides and have essentially no ability to degrade xyloglucan polysaccharides. They release oligosaccharides with two glucose units from non-reducing end of xyloglucan oligosaccharides. On the other hand, GH74 enzymes designated as xyloglucanase; xyloglucan specific endo-&beta;-1,4-glucanases: XEG; and xyloglucan hydrolases: Xgh, (EC [{{EClink}}3.2.1.151 3.2.1.151]), exhibit endo-type activity on xyloglucan from tamarind seed, a readily available and well-investigated xyloglucan <cite>York1993</cite>. Many GH74 xyloglucanases hydrolyze the glycosidic linkage of unbranched glucose residues, but several members including ''Geotrichum sp.'' OXG-RCBH <cite>Yaoi2002</cite>, ''E. nidulans'' OREX <cite>Bauer2005</cite>, and [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=51453''Hypocrea jecorina''] (formerly known as ''Trichoderma reesei'') Cel74A <cite>Desmet2007</cite> accommodate side-chain xylose residues at [[Sub-site nomenclature|subsite]] -1 of the active site<ins class="diffchange diffchange-inline">. As first elucidated by Matsuzawa, Saito, and Yaoi <cite>Matsuzawa2014</cite>, some GH74 members exhibit processivity (multiple catalytic events before chain dissociation) in xyloglucan hydrolysis, which is related to active-site length and the presence of key aromatic residues, and which follows protein phylogeny <cite>Arnal2019</cite></ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Kinetics and Mechanism ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Kinetics and Mechanism ==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Three-dimensional structures ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Three-dimensional structures ==</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Overall structures of GH74 enzymes consist of a tandem repeat of two seven-bladed &beta;-propeller domains. The two domains form a substrate binding cleft at the interface. The catalytic residues are located in the middle of this cleft. One side of the binding cleft of OXG-RCBH is blocked by a so-called 'exo-loop' which is found only in exo-acting enzymes in this family <cite>Yaoi2004</cite>. A crystal structure of a complex with xyloglucan-derived oligosaccharides elucidated the interaction with the side-chains of the substrate by these enzymes <cite>Martinez-Fleites2006</cite>. </div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Overall structures of GH74 enzymes consist of a tandem repeat of two seven-bladed &beta;-propeller domains. The two domains form a substrate binding cleft at the interface. The catalytic residues are located in the middle of this cleft. One side of the binding cleft of OXG-RCBH is blocked by a so-called 'exo-loop' which is found only in exo-acting enzymes in this family <cite>Yaoi2004</cite>. A crystal structure of a complex with xyloglucan-derived oligosaccharides elucidated the interaction with the side-chains of the substrate by these enzymes <cite>Martinez-Fleites2006<ins class="diffchange diffchange-inline"></cite>. A number of GH74 complexes with large xyloglucan oligosaccharides is now known <cite>Arnal2019</ins></cite>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Family Firsts ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Family Firsts ==</div></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l59" >Line 59:</td>
<td colspan="2" class="diff-lineno">Line 59:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#Chhabra2002 pmid=12417345</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#Chhabra2002 pmid=12417345</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#Takada1999 Takada G, Kawagushi T, Yoneda T, Kawasaki M, Sumitani JI, and Arai M. ''Molecular cloning and expression of the celluloytic system of Aspergillus aculeatus'', p. 364-373. ''In'' Ohmiya K, Hayashi K, Sakka K, Kobayashi Y, Karita S, and Kimura T (ed.), ''Genetics, biochemistry and ecology of cellulose degradation.'' 1999 Uni Publishers, Tokyo, Japan, ISBN 4-946450-17-3.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#Takada1999 Takada G, Kawagushi T, Yoneda T, Kawasaki M, Sumitani JI, and Arai M. ''Molecular cloning and expression of the celluloytic system of Aspergillus aculeatus'', p. 364-373. ''In'' Ohmiya K, Hayashi K, Sakka K, Kobayashi Y, Karita S, and Kimura T (ed.), ''Genetics, biochemistry and ecology of cellulose degradation.'' 1999 Uni Publishers, Tokyo, Japan, ISBN 4-946450-17-3.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">#Arnal2019 pmid=31324716</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">#Matsuzawa2014 pmid=24657616</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div></biblio></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div></biblio></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[Category:Glycoside Hydrolase Families|GH074]]</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[Category:Glycoside Hydrolase Families|GH074]]</div></td></tr>
</table>
Harry Brumer
https://www.cazypedia.org/index.php?title=Glycoside_Hydrolase_Family_74&diff=6794&oldid=prev
Takuya Ishida at 23:39, 14 May 2011
2011-05-14T23:39:54Z
<p></p>
<table class="diff diff-contentalign-left diff-editfont-monospace" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en-CA">
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 23:39, 14 May 2011</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l35" >Line 35:</td>
<td colspan="2" class="diff-lineno">Line 35:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Catalytic Residues ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Catalytic Residues ==</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Crystal structure of OXG-RCBH demonstrated that Asp35 and Asp465 are located in the middle of the binding cleft, and their crucial roles in hydrolytic activity were experimentally confirmed by site-directed mutagenesis <cite>Yaoi2004</cite>. However, their identities as [[general acid]] and [[general base]] were not assigned. The corresponding Asp residues in [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1515 ''Clostridium thermocellum''] <del class="diffchange diffchange-inline">xyloglucanase </del>are nicely located between [[Sub-site nomenclature|subsites]] -1 and +1 in the complex structure with xyloglucan-derived oligosaccharides <cite>Martinez-Fleites2006</cite>. </div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Crystal structure of OXG-RCBH demonstrated that Asp35 and Asp465 are located in the middle of the binding cleft, and their crucial roles in hydrolytic activity were experimentally confirmed by site-directed mutagenesis <cite>Yaoi2004</cite>. However, their identities as [[general acid]] and [[general base]] were not assigned. The corresponding Asp residues in <ins class="diffchange diffchange-inline">Xgh74A from </ins>[http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1515 ''Clostridium thermocellum''] are nicely located between [[Sub-site nomenclature|subsites]] -1 and +1 in the complex structure with xyloglucan-derived oligosaccharides <cite>Martinez-Fleites2006</cite>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Three-dimensional structures ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Three-dimensional structures ==</div></td></tr>
</table>
Takuya Ishida
https://www.cazypedia.org/index.php?title=Glycoside_Hydrolase_Family_74&diff=6793&oldid=prev
Takuya Ishida at 11:15, 14 May 2011
2011-05-14T11:15:28Z
<p></p>
<table class="diff diff-contentalign-left diff-editfont-monospace" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en-CA">
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:15, 14 May 2011</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l43" >Line 43:</td>
<td colspan="2" class="diff-lineno">Line 43:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>;First stereochemistry determination:Xeg74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2021 ''Thermobifida fusca''] by <sup>1</sup>H-NMR <cite>Irwin2003</cite>.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>;First stereochemistry determination:Xeg74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2021 ''Thermobifida fusca''] by <sup>1</sup>H-NMR <cite>Irwin2003</cite>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>;First gene cloning:The first gene cloned was AviIII from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=5053 ''Aspergillus aculeatus''] <cite>Takada1999</cite>, and the first xyloglucanase activity was confirmed by the study on EglC from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=5061 ''Aspergillus niger''] <cite>Hasper2002</cite>.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>;First gene cloning:The first gene cloned was AviIII from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=5053 ''Aspergillus aculeatus''] <cite>Takada1999</cite>, and the first xyloglucanase activity was confirmed by the study on EglC from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=5061 ''Aspergillus niger''] <cite>Hasper2002</cite>.</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>;First [[general acid]] residue identification: <del class="diffchange diffchange-inline">OXG-RCBH </del>from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=<del class="diffchange diffchange-inline">203496 </del>''<del class="diffchange diffchange-inline">Geotrichum sp. M128</del>''] <cite><del class="diffchange diffchange-inline">Yaoi2004</del></cite>.</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>;First [[general acid]] residue identification: <ins class="diffchange diffchange-inline">Xgh74A </ins>from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=<ins class="diffchange diffchange-inline">1515 </ins>''<ins class="diffchange diffchange-inline">Clostridium thermocellum</ins>''] <cite><ins class="diffchange diffchange-inline">Martinez-Fleites2006</ins></cite>.</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>;First [[general base]] residue identification: <del class="diffchange diffchange-inline">OXG-RCBH </del>from <del class="diffchange diffchange-inline"> </del>[http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=<del class="diffchange diffchange-inline">203496 </del>''<del class="diffchange diffchange-inline">Geotrichum sp. M128</del>''] <cite><del class="diffchange diffchange-inline">Yaoi2004</del></cite>.</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>;First [[general base]] residue identification: <ins class="diffchange diffchange-inline">Xgh74A </ins>from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=<ins class="diffchange diffchange-inline">1515 </ins>''<ins class="diffchange diffchange-inline">Clostridium thermocellum</ins>''] <cite><ins class="diffchange diffchange-inline">Martinez-Fleites2006</ins></cite>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>;First 3-D structure: OXG-RCBH from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2004</cite>.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>;First 3-D structure: OXG-RCBH from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2004</cite>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
</table>
Takuya Ishida
https://www.cazypedia.org/index.php?title=Glycoside_Hydrolase_Family_74&diff=6792&oldid=prev
Takuya Ishida at 14:59, 13 May 2011
2011-05-13T14:59:04Z
<p></p>
<table class="diff diff-contentalign-left diff-editfont-monospace" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en-CA">
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 14:59, 13 May 2011</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l42" >Line 42:</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Family Firsts ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Family Firsts ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>;First stereochemistry determination:Xeg74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2021 ''Thermobifida fusca''] by <sup>1</sup>H-NMR <cite>Irwin2003</cite>.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>;First stereochemistry determination:Xeg74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2021 ''Thermobifida fusca''] by <sup>1</sup>H-NMR <cite>Irwin2003</cite>.</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>;First gene cloning:AviIII from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=5053 ''Aspergillus aculeatus''] <cite>Takada1999</cite> and EglC from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=5061 ''Aspergillus niger''] <cite>Hasper2002</cite>.</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>;First gene cloning:<ins class="diffchange diffchange-inline">The first gene cloned was </ins>AviIII from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=5053 ''Aspergillus aculeatus''] <cite>Takada1999</cite><ins class="diffchange diffchange-inline">, </ins>and <ins class="diffchange diffchange-inline">the first xyloglucanase activity was confirmed by the study on </ins>EglC from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=5061 ''Aspergillus niger''] <cite>Hasper2002</cite>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>;First [[general acid]] residue identification: OXG-RCBH from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2004</cite>.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>;First [[general acid]] residue identification: OXG-RCBH from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2004</cite>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>;First [[general base]] residue identification: OXG-RCBH from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2004</cite>.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>;First [[general base]] residue identification: OXG-RCBH from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2004</cite>.</div></td></tr>
</table>
Takuya Ishida
https://www.cazypedia.org/index.php?title=Glycoside_Hydrolase_Family_74&diff=6784&oldid=prev
Spencer Williams at 11:25, 12 May 2011
2011-05-12T11:25:54Z
<p></p>
<table class="diff diff-contentalign-left diff-editfont-monospace" data-mw="interface">
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<col class="diff-content" />
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<col class="diff-content" />
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:25, 12 May 2011</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l29" >Line 29:</td>
<td colspan="2" class="diff-lineno">Line 29:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Substrate specificities ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Substrate specificities ==</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Glycoside hydrolases of this family hydrolyze &beta;-1,4-linkages of various glucans. With the exception of Cel74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2336 ''Thermotoga maritima''], all biochemically characterized enzymes are specific toward xyloglucans and/or xyloglucan-oligosaccharides. Cel74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2336 ''Thermotoga maritima''] exhibits the highest activity on barley &beta;-glucan, with relative activity of 20% toward xyloglucan <cite>Chhabra2002</cite>. A wide diversity in the modes of action by GH74 enzymes has been reported. "Oligoxyloglucan reducing end-specific cellobiohydrolase (OXG-RCBH, EC [{{EClink}}3.2.1.150 3.2.1.150])" from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2002</cite> and "oligoxyloglucan reducing end-specific xyloglucanobiohydrolase (OREX)" from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=162425 ''Emericella nidulans''] (formerly known as ''Aspergillus nidulans'') <cite>Bauer2005</cite> are active on only xyloglucan oligosaccharides and have essentially no ability to degrade xyloglucan polysaccharides. They release oligosaccharides with two glucose units from non-reducing end of xyloglucan oligosaccharides. On the other hand, GH74 enzymes designated as xyloglucanase; xyloglucan specific endo-&beta;-1,4-glucanases: XEG; and xyloglucan hydrolases: Xgh, (EC [{{EClink}}3.2.1.151 3.2.1.151]), exhibit endo-type activity on xyloglucan from tamarind seed, a readily available and well-investigated xyloglucan <cite>York1993</cite>. Many GH74 xyloglucanases hydrolyze the glycosidic linkage of unbranched glucose residues, but several members including ''Geotrichum sp.'' OXG-RCBH <cite>Yaoi2002</cite>, ''E. nidulans'' OREX <cite>Bauer2005</cite>, and [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=51453''Hypocrea jecorina''] (formerly known as ''Trichoderma reesei'') Cel74A <cite>Desmet2007</cite> accommodate side-chain xylose residues at [[Sub-site nomenclature|subsite]] -1 of the active site.</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins class="diffchange diffchange-inline">[[</ins>Glycoside hydrolases<ins class="diffchange diffchange-inline">]] </ins>of this family hydrolyze &beta;-1,4-linkages of various glucans. With the exception of Cel74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2336 ''Thermotoga maritima''], all biochemically characterized enzymes are specific toward xyloglucans and/or xyloglucan-oligosaccharides. Cel74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2336 ''Thermotoga maritima''] exhibits the highest activity on barley &beta;-glucan, with relative activity of 20% toward xyloglucan <cite>Chhabra2002</cite>. A wide diversity in the modes of action by GH74 enzymes has been reported. "Oligoxyloglucan reducing end-specific cellobiohydrolase (OXG-RCBH, EC [{{EClink}}3.2.1.150 3.2.1.150])" from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2002</cite> and "oligoxyloglucan reducing end-specific xyloglucanobiohydrolase (OREX)" from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=162425 ''Emericella nidulans''] (formerly known as ''Aspergillus nidulans'') <cite>Bauer2005</cite> are active on only xyloglucan oligosaccharides and have essentially no ability to degrade xyloglucan polysaccharides. They release oligosaccharides with two glucose units from non-reducing end of xyloglucan oligosaccharides. On the other hand, GH74 enzymes designated as xyloglucanase; xyloglucan specific endo-&beta;-1,4-glucanases: XEG; and xyloglucan hydrolases: Xgh, (EC [{{EClink}}3.2.1.151 3.2.1.151]), exhibit endo-type activity on xyloglucan from tamarind seed, a readily available and well-investigated xyloglucan <cite>York1993</cite>. Many GH74 xyloglucanases hydrolyze the glycosidic linkage of unbranched glucose residues, but several members including ''Geotrichum sp.'' OXG-RCBH <cite>Yaoi2002</cite>, ''E. nidulans'' OREX <cite>Bauer2005</cite>, and [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=51453''Hypocrea jecorina''] (formerly known as ''Trichoderma reesei'') Cel74A <cite>Desmet2007</cite> accommodate side-chain xylose residues at [[Sub-site nomenclature|subsite]] -1 of the active site.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Kinetics and Mechanism ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Kinetics and Mechanism ==</div></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l35" >Line 35:</td>
<td colspan="2" class="diff-lineno">Line 35:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Catalytic Residues ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Catalytic Residues ==</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Crystal structure of OXG-RCBH demonstrated that Asp35 and Asp465 are located in the middle of the binding cleft, and <del class="diffchange diffchange-inline">its </del>crucial roles in hydrolytic activity were experimentally confirmed by site-directed mutagenesis <cite>Yaoi2004</cite>. The corresponding Asp residues in [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1515 ''Clostridium thermocellum''] xyloglucanase are nicely located between [[Sub-site nomenclature|subsites]] -1 and +1 in the complex structure with xyloglucan-derived oligosaccharides <cite>Martinez-Fleites2006</cite>.</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Crystal structure of OXG-RCBH demonstrated that Asp35 and Asp465 are located in the middle of the binding cleft, and <ins class="diffchange diffchange-inline">their </ins>crucial roles in hydrolytic activity were experimentally confirmed by site-directed mutagenesis <cite>Yaoi2004</cite><ins class="diffchange diffchange-inline">. However, their identities as [[general acid]] and [[general base]] were not assigned</ins>. The corresponding Asp residues in [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1515 ''Clostridium thermocellum''] xyloglucanase are nicely located between [[Sub-site nomenclature|subsites]] -1 and +1 in the complex structure with xyloglucan-derived oligosaccharides <cite>Martinez-Fleites2006</cite>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Three-dimensional structures ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Three-dimensional structures ==</div></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l43" >Line 43:</td>
<td colspan="2" class="diff-lineno">Line 43:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>;First stereochemistry determination:Xeg74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2021 ''Thermobifida fusca''] by <sup>1</sup>H-NMR <cite>Irwin2003</cite>.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>;First stereochemistry determination:Xeg74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2021 ''Thermobifida fusca''] by <sup>1</sup>H-NMR <cite>Irwin2003</cite>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>;First gene cloning:AviIII from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=5053 ''Aspergillus aculeatus''] <cite>Takada1999</cite> and EglC from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=5061 ''Aspergillus niger''] <cite>Hasper2002</cite>.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>;First gene cloning:AviIII from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=5053 ''Aspergillus aculeatus''] <cite>Takada1999</cite> and EglC from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=5061 ''Aspergillus niger''] <cite>Hasper2002</cite>.</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>;First general acid residue identification: OXG-RCBH from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2004</cite>.</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>;First <ins class="diffchange diffchange-inline">[[</ins>general acid<ins class="diffchange diffchange-inline">]] </ins>residue identification: OXG-RCBH from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2004</cite>.</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>;First general base residue identification: OXG-RCBH from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2004</cite>.</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>;First <ins class="diffchange diffchange-inline">[[</ins>general base<ins class="diffchange diffchange-inline">]] </ins>residue identification: OXG-RCBH from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2004</cite>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>;First 3-D structure: OXG-RCBH from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2004</cite>.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>;First 3-D structure: OXG-RCBH from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2004</cite>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
</table>
Spencer Williams
https://www.cazypedia.org/index.php?title=Glycoside_Hydrolase_Family_74&diff=6676&oldid=prev
Harry Brumer: fixed Yaoi2004 reference issues
2011-05-09T07:37:21Z
<p>fixed Yaoi2004 reference issues</p>
<table class="diff diff-contentalign-left diff-editfont-monospace" data-mw="interface">
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 07:37, 9 May 2011</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l35" >Line 35:</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Catalytic Residues ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Catalytic Residues ==</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Crystal structure of OXG-RCBH demonstrated that Asp35 and Asp465 are located in the middle of the binding cleft, and its crucial roles in hydrolytic activity were experimentally confirmed by site-directed mutagenesis <cite><del class="diffchange diffchange-inline">Yaoi2004b</del></cite>. The corresponding Asp residues in [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1515 ''Clostridium thermocellum''] xyloglucanase are nicely located between [[Sub-site nomenclature|subsites]] -1 and +1 in the complex structure with xyloglucan-derived oligosaccharides <cite>Martinez-Fleites2006</cite>.</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Crystal structure of OXG-RCBH demonstrated that Asp35 and Asp465 are located in the middle of the binding cleft, and its crucial roles in hydrolytic activity were experimentally confirmed by site-directed mutagenesis <cite><ins class="diffchange diffchange-inline">Yaoi2004</ins></cite>. The corresponding Asp residues in [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1515 ''Clostridium thermocellum''] xyloglucanase are nicely located between [[Sub-site nomenclature|subsites]] -1 and +1 in the complex structure with xyloglucan-derived oligosaccharides <cite>Martinez-Fleites2006</cite>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Three-dimensional structures ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Three-dimensional structures ==</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Overall structures of GH74 enzymes consist of a tandem repeat of two seven-bladed &beta;-propeller domains. The two domains form a substrate binding cleft at the interface. The catalytic residues are located in the middle of this cleft. One side of the binding cleft of OXG-RCBH is blocked by a so-called 'exo-loop' which is found only in exo-acting enzymes in this family <cite><del class="diffchange diffchange-inline">Yaoi2004b</del></cite>. A crystal structure of a complex with xyloglucan-derived oligosaccharides elucidated the interaction with the side-chains of the substrate by these enzymes <cite>Martinez-Fleites2006</cite>. </div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Overall structures of GH74 enzymes consist of a tandem repeat of two seven-bladed &beta;-propeller domains. The two domains form a substrate binding cleft at the interface. The catalytic residues are located in the middle of this cleft. One side of the binding cleft of OXG-RCBH is blocked by a so-called 'exo-loop' which is found only in exo-acting enzymes in this family <cite><ins class="diffchange diffchange-inline">Yaoi2004</ins></cite>. A crystal structure of a complex with xyloglucan-derived oligosaccharides elucidated the interaction with the side-chains of the substrate by these enzymes <cite>Martinez-Fleites2006</cite>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Family Firsts ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Family Firsts ==</div></td></tr>
</table>
Harry Brumer
https://www.cazypedia.org/index.php?title=Glycoside_Hydrolase_Family_74&diff=6674&oldid=prev
Katsuro Yaoi at 04:17, 9 May 2011
2011-05-09T04:17:37Z
<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 04:17, 9 May 2011</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1" >Line 1:</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption --></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption --></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>{{<del class="diffchange diffchange-inline">UnderConstruction</del>}}</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>{{<ins class="diffchange diffchange-inline">CuratorApproved</ins>}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* [[Author]]: ^^^Katsuro Yaoi^^^ and ^^^Takuya Ishida^^^</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* [[Author]]: ^^^Katsuro Yaoi^^^ and ^^^Takuya Ishida^^^</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* [[Responsible Curator]]: ^^^Katsuro Yaoi^^^</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* [[Responsible Curator]]: ^^^Katsuro Yaoi^^^</div></td></tr>
</table>
Katsuro Yaoi
https://www.cazypedia.org/index.php?title=Glycoside_Hydrolase_Family_74&diff=6673&oldid=prev
Katsuro Yaoi at 02:23, 9 May 2011
2011-05-09T02:23:09Z
<p></p>
<table class="diff diff-contentalign-left diff-editfont-monospace" data-mw="interface">
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 02:23, 9 May 2011</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l32" >Line 32:</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Kinetics and Mechanism ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Kinetics and Mechanism ==</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Family 74 enzymes are [[inverting]] enzymes, as shown by NMR analysis on Xeg74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2021 ''Thermobifida fusca''] <cite>Irwin2003<del class="diffchange diffchange-inline"></cite> and XEG from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=203496 ''Geotrichum sp. M128''] <cite>Yaoi2004a</del></cite>.</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Family 74 enzymes are [[inverting]] enzymes, as shown by NMR analysis on Xeg74 from [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2021 ''Thermobifida fusca''] <cite>Irwin2003</cite>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Catalytic Residues ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Catalytic Residues ==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#Yaoi2002 pmid=12374797</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#Yaoi2002 pmid=12374797</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#Irwin2003 pmid=12846842</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#Irwin2003 pmid=12846842</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>#<del class="diffchange diffchange-inline">Yaoi2004a pmid=14987996</del></div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>#<ins class="diffchange diffchange-inline">Yaoi2004 </ins>pmid=15242597</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del class="diffchange diffchange-inline">#Yaoi2004b </del>pmid=15242597</div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#Martinez-Fleites2006 pmid=16772298</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#Martinez-Fleites2006 pmid=16772298</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#York1993 pmid=8252539</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>#York1993 pmid=8252539</div></td></tr>
</table>
Katsuro Yaoi