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Difference between revisions of "Glycoside Hydrolases"

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'''The text below is a template to help you create a consistent layout for GH entries.  To get an idea of what to put in each field, save this edit and have a look at any of the GH families by following this link: [[:Category:Glycoside Hydrolase Families]]''' ''(TIP: Right click with your mouse and open the link in a new browser window...)''
 
 
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== Overview ==
 
== Overview ==
  
 
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Glycoside hydrolases are enzymes that catalyze the hydrolysis of the glycosidic linkage of glycosides, leading to the formation of a simple sugar and the corresponding alglycon. Glycoside hydrolases are also referred to as glycosidases. Glycoside hydrolases are known that can catalyze the hydrolysis of O-, N- and S-linked glycosides.
  
 
== Classification ==
 
== Classification ==
  
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Glycoside hydrolases can be classified in many different ways. The following paragraphs list several different ways, the utility of which depends on the context in which the classification is made and used.
  
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''exo''- and ''endo''- refers to the ability of a glycoside hydrolase to cleave a substrate at the end (most frequently, but not always the non-reducing end) or within the middle of a chain. For example, most cellulases are ''endo''-acting, whereas LacZ β-galactosidase from ''E. coli'' is ''exo''-acting.
  
 
== Mechanism ==
 
== Mechanism ==

Revision as of 01:50, 21 August 2008


Overview

Glycoside hydrolases are enzymes that catalyze the hydrolysis of the glycosidic linkage of glycosides, leading to the formation of a simple sugar and the corresponding alglycon. Glycoside hydrolases are also referred to as glycosidases. Glycoside hydrolases are known that can catalyze the hydrolysis of O-, N- and S-linked glycosides.

Classification

Glycoside hydrolases can be classified in many different ways. The following paragraphs list several different ways, the utility of which depends on the context in which the classification is made and used.

exo- and endo- refers to the ability of a glycoside hydrolase to cleave a substrate at the end (most frequently, but not always the non-reducing end) or within the middle of a chain. For example, most cellulases are endo-acting, whereas LacZ β-galactosidase from E. coli is exo-acting.

Mechanism

Sequence-based classification

Glycoside Hydrolase Firsts

First sterochemistry determination
Cite some reference here, with a short explanation [1].
First catalytic nucleophile identification
First general acid/base residue identification
First 3-D structure

References

  1. Comfort DA, Bobrov KS, Ivanen DR, Shabalin KA, Harris JM, Kulminskaya AA, Brumer H, and Kelly RM. (2007). Biochemical analysis of Thermotoga maritima GH36 alpha-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D glycoside hydrolases. Biochemistry. 2007;46(11):3319-30. DOI:10.1021/bi061521n | PubMed ID:17323919 [1]

[[Category:Glycoside Hydrolase Families]]