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User:Masahiro Nakajima

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Masahiro Nakajima received his Ph.D. from the Graduate School of Agricultural and Life Science, The University of Tokyo in 2006. He joined the group of Dr. Motomitsu Kitaoka as a postdoctoral fellow (2006-2010). He moved to Iwate Biotechnology Research Center as a researcher (2010-2012). He is an assistant professor in Taguchi Laboratory at Department of Applied Biological Science, Tokyo University of Science (2012-). His research currently focuses on structures and functions of carbohydrate-active enzymes acting on unique sugar chains such as β-1,2-glucan. He acts as a Responsible Curator of Glycoside Hydrolase Families GH144 and GH162. He determined the functions and/or crystal structures of

  • GH3 β-Glucosidases [1, 2, 3]
  • GH16 β-1,3-Glucanase [4]
  • GH38 α-Mannosidase [5]
  • GH57 4-α-Glucanotransferase [6]
  • GH94 1,2-β-Oligoglucan phosphorylases [7, 8]
  • GH112 D-Galactosyl-β-1,4-L-rhamnose phosphorylase and β-1,3-galactosyl-N-acetylhexosamine phosphorylases [9, 10, 11, 12]
  • GH144 Bacterial β-1,2-Glucanases [13, 14]
  • GH162 Fungal β-1,2-Glucanase [15]



  1. Nakajima M, Yamashita T, Takahashi M, Nakano Y, and Takeda T. (2012) Identification, cloning, and characterization of β-glucosidase from Ustilago esculenta. Appl Microbiol Biotechnol. 93, 1989-98. DOI:10.1007/s00253-011-3538-2 | PubMed ID:21850431 | HubMed [Nakajima2012a]
  2. Nakajima M, Yoshida R, Miyanaga A, Abe K, Takahashi Y, Sugimoto N, Toyoizumi H, Nakai H, Kitaoka M, and Taguchi H. (2016) Functional and Structural Analysis of a β-Glucosidase Involved in β-1,2-Glucan Metabolism in Listeria innocua. PLoS One. 11, e0148870. DOI:10.1371/journal.pone.0148870 | PubMed ID:26886583 | HubMed [Nakajima2016]
  3. Ishiguro R, Tanaka N, Abe K, Nakajima M, Maeda T, Miyanaga A, Takahashi Y, Sugimoto N, Nakai H, and Taguchi H. (2017) Function and structure relationships of a β-1,2-glucooligosaccharide-degrading β-glucosidase. FEBS Lett. 591, 3926-3936. DOI:10.1002/1873-3468.12911 | PubMed ID:29131329 | HubMed [Ishiguro2017]
  4. Nakajima M, Yamashita T, Takahashi M, Nakano Y, and Takeda T. (2012) A novel glycosylphosphatidylinositol-anchored glycoside hydrolase from Ustilago esculenta functions in β-1,3-glucan degradation. Appl Environ Microbiol. 78, 5682-9. DOI:10.1128/AEM.00483-12 | PubMed ID:22685137 | HubMed [Nakajima2012b]
  5. Nakajima M, Imamura H, Shoun H, and Wakagi T. (2003) Unique metal dependency of cytosolic alpha-mannosidase from Thermotoga maritima, a hyperthermophilic bacterium. Arch Biochem Biophys. 415, 87-93. DOI:10.1016/s0003-9861(03)00222-4 | PubMed ID:12801516 | HubMed [Nakajima2003]
  6. Nakajima M, Imamura H, Shoun H, Horinouchi S, and Wakagi T. (2004) Transglycosylation activity of Dictyoglomus thermophilum amylase A. Biosci Biotechnol Biochem. 68, 2369-73. DOI:10.1271/bbb.68.2369 | PubMed ID:15564678 | HubMed [Nakajima2004]
  7. Nakajima M, Tanaka N, Furukawa N, Nihira T, Kodutsumi Y, Takahashi Y, Sugimoto N, Miyanaga A, Fushinobu S, Taguchi H, and Nakai H. (2017) Mechanistic insight into the substrate specificity of 1,2-β-oligoglucan phosphorylase from Lachnoclostridium phytofermentans. Sci Rep. 7, 42671. DOI:10.1038/srep42671 | PubMed ID:28198470 | HubMed [Nakajima2017]
  8. Nakajima M, Toyoizumi H, Abe K, Nakai H, Taguchi H, and Kitaoka M. (2014) 1,2-β-Oligoglucan phosphorylase from Listeria innocua. PLoS One. 9, e92353. DOI:10.1371/journal.pone.0092353 | PubMed ID:24647662 | HubMed [Nakajima2014]
  9. Nakajima M, Nishimoto M, and Kitaoka M. (2009) Characterization of three beta-galactoside phosphorylases from Clostridium phytofermentans: discovery of d-galactosyl-beta1->4-l-rhamnose phosphorylase. J Biol Chem. 284, 19220-7. DOI:10.1074/jbc.M109.007666 | PubMed ID:19491100 | HubMed [Nakajima2009a]
  10. Nakajima M, Nishimoto M, and Kitaoka M. (2009) Characterization of beta-1,3-galactosyl-N-acetylhexosamine phosphorylase from Propionibacterium acnes. Appl Microbiol Biotechnol. 83, 109-15. DOI:10.1007/s00253-008-1838-y | PubMed ID:19132369 | HubMed [Nakajima2009b]
  11. Nakajima M and Kitaoka M. (2008) Identification of lacto-N-Biose I phosphorylase from Vibrio vulnificus CMCP6. Appl Environ Microbiol. 74, 6333-7. DOI:10.1128/AEM.02846-07 | PubMed ID:18723650 | HubMed [Nakajima2008a]
  12. Nakajima M, Nihira T, Nishimoto M, and Kitaoka M. (2008) Identification of galacto-N-biose phosphorylase from Clostridium perfringens ATCC13124. Appl Microbiol Biotechnol. 78, 465-71. DOI:10.1007/s00253-007-1319-8 | PubMed ID:18183385 | HubMed [Nakajima2008b]
  13. Abe K, Nakajima M, Yamashita T, Matsunaga H, Kamisuki S, Nihira T, Takahashi Y, Sugimoto N, Miyanaga A, Nakai H, Arakawa T, Fushinobu S, and Taguchi H. (2017) Biochemical and structural analyses of a bacterial endo-β-1,2-glucanase reveal a new glycoside hydrolase family. J Biol Chem. 292, 7487-7506. DOI:10.1074/jbc.M116.762724 | PubMed ID:28270506 | HubMed [Abe2017]
  14. Shimizu H, Nakajima M, Miyanaga A, Takahashi Y, Tanaka N, Kobayashi K, Sugimoto N, Nakai H, and Taguchi H. (2018) Characterization and Structural Analysis of a Novel exo-Type Enzyme Acting on β-1,2-Glucooligosaccharides from Parabacteroides distasonis. Biochemistry. 57, 3849-3860. DOI:10.1021/acs.biochem.8b00385 | PubMed ID:29763309 | HubMed [Shimizu2018]
  15. Tanaka N, Nakajima M, Narukawa-Nara M, Matsunaga H, Kamisuki S, Aramasa H, Takahashi Y, Sugimoto N, Abe K, Terada T, Miyanaga A, Yamashita T, Sugawara F, Kamakura T, Komba S, Nakai H, and Taguchi H. (2019) Identification, characterization, and structural analyses of a fungal endo-β-1,2-glucanase reveal a new glycoside hydrolase family. J Biol Chem. 294, 7942-7965. DOI:10.1074/jbc.RA118.007087 | PubMed ID:30926603 | HubMed [Tanaka2019]
All Medline abstracts: PubMed | HubMed