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User:Motomitsu Kitaoka

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Motomitsu Kitaoka is Leader of Enzyme Laboratory, National Food Research Institute, National Agriculture and Food Research Organization, Japan. He obtained Ph. D. from The University of Tokyo in 1993. He has been working on phosphorolytic enzymes for more than 20 years, especially working on developing new procedure to produce oligosaccharides practically such as a kg-scale preparation of Gal-β1,3-GlcNAc [1]. He also converted an inverting GH into glycosynthase [2]. Out of research on carbohydrate, he developed a simple (he believes it the simplest) protocol to introduce random mutations in DNA [3].

  1. Nishimoto M and Kitaoka M. (2007). Practical preparation of lacto-N-biose I, a candidate for the bifidus factor in human milk. Biosci Biotechnol Biochem. 2007;71(8):2101-4. DOI:10.1271/bbb.70320 | PubMed ID:17690443 [Nishimoto2007]
  2. Honda Y and Kitaoka M. (2006). The first glycosynthase derived from an inverting glycoside hydrolase. J Biol Chem. 2006;281(3):1426-31. DOI:10.1074/jbc.M511202200 | PubMed ID:16301312 [Honda2006]
  3. Fujii R, Kitaoka M, and Hayashi K. (2006). Error-prone rolling circle amplification: the simplest random mutagenesis protocol. Nat Protoc. 2006;1(5):2493-7. DOI:10.1038/nprot.2006.403 | PubMed ID:17406496 [Fujii2006]

All Medline abstracts: PubMed