User:Masahiro Nakajima

Masahiro Nakajima received his Ph.D. from the Graduate School of Agricultural and Life Science, The University of Tokyo in 2006. He joined the group of Dr. Motomitsu Kitaoka as a postdoctoral fellow (2006–2010). He moved to Iwate Biotechnology Research Center as a researcher (2010–2012). He was an assistant professor in Taguchi Laboratory (2012–2020) and is currently an associate professor in his own laboratory (2020-) at Department of Applied Biological Science, Tokyo University of Science. His research currently focuses on structures and functions of carbohydrate-active enzymes acting on unique sugar chains such as β-1,2-glucan. He acts as a Responsible Curator of Glycoside Hydrolase Families GH144, GH162, GH186 and GH189 and also created clan GH-S. He determined the functions and/or crystal structures of

• GH3 β-Glucosidases [1, 2, 3]
• GH16 β-1,3-Glucanase [4]
• GH35 β-1,2-Glucosyltransglycosylase [5] (new EC number)
• GH38 α-Mannosidase [6]
• GH57 4-α-Glucanotransferase [7]
• GH94 1,2-β-Oligoglucan phosphorylases [8, 9] (new EC number)
• GH112 D-Galactosyl-β-1,4-L-rhamnose phosphorylase [10] (new EC number) and β-1,3-galactosyl-N-acetylhexosamine phosphorylases [10, 11, 12, 13]
• GH144 Bacterial β-1,2-glucanases [14] (family created) and sophorosylhydrolase [15] (new EC number)
• GH162 Fungal β-1,2-glucanase [16] (family created) (clan GH-S created)
• GH186 E. coli β-1,2-glucanase [17] (family created) and α-1,6-cyclized β-1,2-glucohexadecaose synthase from Xanthomonas campestris pv. campestris (new EC number should be given to the enzyme.)[18]
• GH189 Cyclic β-1,2-glucan synthase (transglycosylase domain) [19] (family created) (new EC number)

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7. Nakajima M, Imamura H, Shoun H, Horinouchi S, and Wakagi T. (2004). Transglycosylation activity of Dictyoglomus thermophilum amylase A. Biosci Biotechnol Biochem. 2004;68(11):2369-73. DOI:10.1271/bbb.68.2369 |
16. Tanaka N, Nakajima M, Narukawa-Nara M, Matsunaga H, Kamisuki S, Aramasa H, Takahashi Y, Sugimoto N, Abe K, Terada T, Miyanaga A, Yamashita T, Sugawara F, Kamakura T, Komba S, Nakai H, and Taguchi H. (2019). Identification, characterization, and structural analyses of a fungal endo-β-1,2-glucanase reveal a new glycoside hydrolase family. J Biol Chem. 2019;294(19):7942-7965. DOI:10.1074/jbc.RA118.007087 |
17. Motouchi S, Kobayashi K, Nakai H, and Nakajima M. (2023). Identification of enzymatic functions of osmo-regulated periplasmic glucan biosynthesis proteins from Escherichia coli reveals a novel glycoside hydrolase family. Commun Biol. 2023;6(1):961. DOI:10.1038/s42003-023-05336-6 |