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Difference between revisions of "Transglycosylases"
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[[Image:transglycosylation.png|600px|center]] | [[Image:transglycosylation.png|600px|center]] | ||
<center>'''Generalized mechanism of a transglycosylase. Enzymatic cleavage of a substrate through a [[classical Koshland retaining mechanism]] results in formation of a glycosyl enzyme intermediate. This can partition to react with either water to cause hydrolysis (glycoside hydrolase activity) or to an alternative acceptor, often a sugar, to cause transglycosylation (transglycosylase activity).'''</center> | <center>'''Generalized mechanism of a transglycosylase. Enzymatic cleavage of a substrate through a [[classical Koshland retaining mechanism]] results in formation of a glycosyl enzyme intermediate. This can partition to react with either water to cause hydrolysis (glycoside hydrolase activity) or to an alternative acceptor, often a sugar, to cause transglycosylation (transglycosylase activity).'''</center> | ||
+ | |||
+ | ===Families=== | ||
+ | |||
+ | GH families with notable transglycosylase activity include: | ||
+ | [[GH1]], for example LacZ β-galactosidase converts lactose to allolactose. | ||
+ | [[GH13]], for example cyclodextran glucanotransferases. | ||
+ | [[GH16]], for example xyloglucan endotransglycosylases. | ||
+ | |||
== References == | == References == |
Revision as of 03:26, 3 May 2013
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- Author: Spencer Williams
- Responsible Curator: Spencer Williams
Overview
Transglycosylases are a class of GH enzymes that can catalyze the transformation of one glycoside to another. That is, these enzymes catalyze the interchange of an aglycon of a glycoside. Mechanistically, transglycosylases utilize the same mechanism as certain retaining glycoside hydrolases. Thus, reaction of the nucleophile of a retaining glycoside hydrolase with a substrate gives a glycosyl-enzyme intermediate that can be intercepted either by water to give the hydrolysis product, or by another acceptor (often another carbohydrate alcohol), to give a new glycoside or oligosaccharide [1]. Some transglycosidases possess substantial glycoside hydrolase activity, and some glycoside hydrolases possess transglycosylases activity. Indeed, in many cases it is unclear what the major role of an enzyme that possesses both activities may be. Transglycosylases are classified as glycoside hydrolases into various GH families on the basis of sequence similarity.
Families
GH families with notable transglycosylase activity include: GH1, for example LacZ β-galactosidase converts lactose to allolactose. GH13, for example cyclodextran glucanotransferases. GH16, for example xyloglucan endotransglycosylases.