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Difference between revisions of "Glycoside Hydrolase Family 12"
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== Three-dimensional structures == | == Three-dimensional structures == | ||
− | Enzymes from family GH12 adopt a “jelly-roll” fold with two twisted, mostly antiparallel β-sheets stacking on top of each other and enclosing a long substrate-binding groove located on the concave face of the sheet. A single α-helix packs against the convex surface of the outer β-sheet. GH12 members of bacterial origin feature an additional two-stranded β-sheet, stabilized by a disulphide bridge, located at the rim of the substrate binding groove and providing an additional substrate binding platform as compared to the eukaryotic counterparts. The first structural template provided for family GH12 was that of ''Streptomyces lividans'' CelB2 in the apo-form <cite>Sulzenbacher1997</cite> and in the form of a glycosyl-enzyme intermediate <cite>Sulzenbacher1997</cite>. This first structure confirmed previous predictions, based on hydrophobic cluster analysis <cite>Torronen1993</cite>, that GH12 enzymes would display significant structural similarity with family GH11 xylanases and gave rise to the creation of clan GH-C, which so far comprises only the two above families. The first structure of a fungal GH12 enzyme was provided shortly after for Trichoderma reesei (formerly Hypocrea jecorina) Cel12A <cite>Sandgren2001</cite>. To date structural models, both in the apo-form and in complex with oligosaccharides, are available for sixteen family members. From the different complex structures it can be seen that the substrate-binding groove of family GH12 enzymes harbours six binding sites, from -4 to +2, lined by numerous aromatic residues providing staking platforms for the individual pyranose units. This is consistent with the endoglucanase activity of these enzymes, exhibiting low catalytic efficiency towards short chain substrates <cite>Karlsson2002</cite>. The [[catalytic nucleophile]] Glu120 (S. lividans CelB2 numbering) and the [[ general acid/base]] Glu203 are in close proximity of an invariant aspartate, which might have an important role in catalysis and the constellation of this catalytic triad is reminiscent of the catalytic centre of family GH7 enzymes. Like in family [[GH7]] enzymes the acid/base residues of family GH12 members protonate syn to the pyranoside 0-5-C-1 bond. | + | Enzymes from family GH12 adopt a “jelly-roll” fold with two twisted, mostly antiparallel β-sheets stacking on top of each other and enclosing a long substrate-binding groove located on the concave face of the sheet. A single α-helix packs against the convex surface of the outer β-sheet. GH12 members of bacterial origin feature an additional two-stranded β-sheet, stabilized by a disulphide bridge, located at the rim of the substrate binding groove and providing an additional substrate binding platform as compared to the eukaryotic counterparts. The first structural template provided for family GH12 was that of ''Streptomyces lividans'' CelB2 in the apo-form <cite>Sulzenbacher1997</cite> and in the form of a glycosyl-enzyme intermediate <cite>Sulzenbacher1997</cite>. This first structure confirmed previous predictions, based on hydrophobic cluster analysis <cite>Torronen1993</cite>, that GH12 enzymes would display significant structural similarity with family [[GH11]] xylanases and gave rise to the creation of clan GH-C, which so far comprises only the two above families. The first structure of a fungal GH12 enzyme was provided shortly after for ''Trichoderma reesei'' (formerly ''Hypocrea jecorina'') Cel12A <cite>Sandgren2001</cite>. To date structural models, both in the apo-form and in complex with oligosaccharides, are available for sixteen family members. From the different complex structures it can be seen that the substrate-binding groove of family GH12 enzymes harbours six binding sites, from -4 to +2, lined by numerous aromatic residues providing staking platforms for the individual pyranose units. This is consistent with the endoglucanase activity of these enzymes, exhibiting low catalytic efficiency towards short chain substrates <cite>Karlsson2002</cite>. The [[catalytic nucleophile]] Glu120 (S. lividans CelB2 numbering) and the [[ general acid/base]] Glu203 are in close proximity of an invariant aspartate, which might have an important role in catalysis and the constellation of this catalytic triad is reminiscent of the catalytic centre of family GH7 enzymes. Like in family [[GH7]] enzymes the acid/base residues of family GH12 members protonate syn to the pyranoside 0-5-C-1 bond. |
− | For xyloglucan specific enzymes of the family, structural and functional studies of xyloglucanase XG12 from Bacillus licheniformis | + | For xyloglucan specific enzymes of the family, structural and functional studies of xyloglucanase XG12 from ''Bacillus licheniformis'' <cite>Gloster2007</cite> and the xyloglucan specific endo-b-1,4-glucanase XEG1 from ''Aspergillus aculeatus KSM 50'' <cite>Yoshizawa2012</cite> have shown that α-1,6-xylose substitutions can be tolerated at subsites -3, -2, +1 and +2. Certain GH12 enzymes exhibit β-1,3-1,4-glucanase activity and the crystal structure of ''Humicola grisea'' Cel12A in complex with a mixed linkage oligosaccharide arising from a transglycosylation reaction revealed that the β-1,3-linkage can be accommodated with ease between subsites -3 and -2 <cite>Sandgren2004</cite>. Speculations had been forwarded that a long loop crossing the substrate-binding groove at its reducing end, known as the “cord” and conserved in all clan GH-C members, might undergo conformational changes upon substrate binding <cite>Torronen1994</cite>, but the available GH12 complex structures support the view that the role of this loop is to deliver residues for substrate binding. |
− | The crystal structure of Aspergillus aculeatus XEG1 in complex with the glycoside hydrolase inhibitory protein from carrot. EDGP, reveals an original mode of inhibition, distinct from the one observed previously for the inhibition of a family 11 xylanase by a wheat protein | + | The crystal structure of Aspergillus aculeatus XEG1 in complex with the glycoside hydrolase inhibitory protein from carrot. EDGP, reveals an original mode of inhibition, distinct from the one observed previously for the inhibition of a family 11 xylanase by a wheat protein <cite>Payan2004</cite>. In the XEG1-GHIP complex the inhibitor mimics a xyloglucan substrate and inserts two conserved arginine residues into the active site, which establish salt bridges with the carboxylate groups of the catalytic centre <cite>Yoshizawa2012</cite>). |
− | Due to their commercial interest in bioprocessing applications, GH12 endo-b-1,4-glucanases from Humicola grisea | + | Due to their commercial interest in bioprocessing applications, GH12 endo-b-1,4-glucanases from Humicola grisea <cite>Sandgren2003a</cite>, Trichoderma reesei <cite>Sandgren2003a</cite> Sandgren 2003b), Rhodothermus marinus (Kapoor et al, 2008), Pyrococcus furiosus (Kim et al, 2012) and Thermotoga maritima (Cheng et al, 2012) have been extensively engineered and the structural studies of the resulting variants have provided valuable insight into structural features governing enzyme activity and stability. |
Revision as of 06:58, 28 July 2015
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
- Authors: ^^^Mats Sandgren^^^ and ^^^Gerlind Sulzenbacher^^^
- Responsible Curator: ^^^Gerlind Sulzenbacher^^^
Glycoside Hydrolase Family GH12 | |
Clan | GH-C |
Mechanism | retaining |
Active site residues | known |
CAZy DB link | |
http://www.cazy.org/GH12.html |
Substrate specificities
The substrate specificities found among the glycoside hydrolases of family 12 are: endo-β-1,4-glucanase (EC 3.2.1.4), xyloglucan endo-hydrolase (EC 3.2.1.151), endo-β-1,3-1,4-glucanase (EC 3.2.1.73). Xyloglucan endo-transglycosylase (XET, EC 2.4.1.207) activity has been observed in a single fungal GH12 member (GenBank AAN89225.1) using a XET-specific screen [1], although this may represent a side activity of a predominant xyloglucan endo-hydrolase [2, 3].
Kinetics and Mechanism
GH12 enzymes are retaining enzymes, as first shown by NMR studies [4] on endoglucanase 3 from Humicola insolens, and is believed to follow a classical Koshland double-displacement mechanism in which a glycosyl-enzyme intermediate is formed and subsequently this intermediate is hydrolysed via oxocarbenium-ion transition states. No detailed studies involving both steady state and pre-steady state kinetic have yet been reported for GH12.
Catalytic Residues
The catalytic nucleophile and the general acid/base catalyst of GH12 enzymes was initialy predicted by sequence homology to the xylanase members of GH11, a glycoside hydrolase family where the catalytic nucleophile was first identified in the Bacillus circulans endo-xylanase through trapping of the 2-deoxy-2-fluoroxylobiosyl-enzyme intermediate and subsequent peptide mapping via LC-MS/MS technologies [5]. GH11 and GH12 together form clan GH-C. The prediction of the catalytic nucleophile and the general acid/base of GH family 12 enzymes was later confirmed to be correct when the first three dimensional structure of a GH family 12 enzyme was determined, that of Streptomyces Lividans CelB [6]. The catalytic nucleophile was subsequentialy confirmed in Streptomyces Lividans CelB to be Glu 120 by using the same labeling strategy used for detecting the catalytic nucleophile of GH family 11 [7].
Three-dimensional structures
Enzymes from family GH12 adopt a “jelly-roll” fold with two twisted, mostly antiparallel β-sheets stacking on top of each other and enclosing a long substrate-binding groove located on the concave face of the sheet. A single α-helix packs against the convex surface of the outer β-sheet. GH12 members of bacterial origin feature an additional two-stranded β-sheet, stabilized by a disulphide bridge, located at the rim of the substrate binding groove and providing an additional substrate binding platform as compared to the eukaryotic counterparts. The first structural template provided for family GH12 was that of Streptomyces lividans CelB2 in the apo-form [6] and in the form of a glycosyl-enzyme intermediate [6]. This first structure confirmed previous predictions, based on hydrophobic cluster analysis [8], that GH12 enzymes would display significant structural similarity with family GH11 xylanases and gave rise to the creation of clan GH-C, which so far comprises only the two above families. The first structure of a fungal GH12 enzyme was provided shortly after for Trichoderma reesei (formerly Hypocrea jecorina) Cel12A [9]. To date structural models, both in the apo-form and in complex with oligosaccharides, are available for sixteen family members. From the different complex structures it can be seen that the substrate-binding groove of family GH12 enzymes harbours six binding sites, from -4 to +2, lined by numerous aromatic residues providing staking platforms for the individual pyranose units. This is consistent with the endoglucanase activity of these enzymes, exhibiting low catalytic efficiency towards short chain substrates [10]. The catalytic nucleophile Glu120 (S. lividans CelB2 numbering) and the general acid/base Glu203 are in close proximity of an invariant aspartate, which might have an important role in catalysis and the constellation of this catalytic triad is reminiscent of the catalytic centre of family GH7 enzymes. Like in family GH7 enzymes the acid/base residues of family GH12 members protonate syn to the pyranoside 0-5-C-1 bond.
For xyloglucan specific enzymes of the family, structural and functional studies of xyloglucanase XG12 from Bacillus licheniformis [11] and the xyloglucan specific endo-b-1,4-glucanase XEG1 from Aspergillus aculeatus KSM 50 [12] have shown that α-1,6-xylose substitutions can be tolerated at subsites -3, -2, +1 and +2. Certain GH12 enzymes exhibit β-1,3-1,4-glucanase activity and the crystal structure of Humicola grisea Cel12A in complex with a mixed linkage oligosaccharide arising from a transglycosylation reaction revealed that the β-1,3-linkage can be accommodated with ease between subsites -3 and -2 [13]. Speculations had been forwarded that a long loop crossing the substrate-binding groove at its reducing end, known as the “cord” and conserved in all clan GH-C members, might undergo conformational changes upon substrate binding [14], but the available GH12 complex structures support the view that the role of this loop is to deliver residues for substrate binding.
The crystal structure of Aspergillus aculeatus XEG1 in complex with the glycoside hydrolase inhibitory protein from carrot. EDGP, reveals an original mode of inhibition, distinct from the one observed previously for the inhibition of a family 11 xylanase by a wheat protein [15]. In the XEG1-GHIP complex the inhibitor mimics a xyloglucan substrate and inserts two conserved arginine residues into the active site, which establish salt bridges with the carboxylate groups of the catalytic centre [12]).
Due to their commercial interest in bioprocessing applications, GH12 endo-b-1,4-glucanases from Humicola grisea [16], Trichoderma reesei [16] Sandgren 2003b), Rhodothermus marinus (Kapoor et al, 2008), Pyrococcus furiosus (Kim et al, 2012) and Thermotoga maritima (Cheng et al, 2012) have been extensively engineered and the structural studies of the resulting variants have provided valuable insight into structural features governing enzyme activity and stability.
Family Firsts
- First sterochemistry determination
- Humicola insolens endoglucanase 3 by NMR [4].
- First catalytic nucleophile identification
- .
- First general acid/base residue identification
- .
- First 3-D structure
- .
References
-
Nielsen, RI. (2002) Microbial xyloglucan endotransglycosylase (XET) Patent US6448056.
- Gilbert HJ, Stålbrand H, and Brumer H. (2008). How the walls come crumbling down: recent structural biochemistry of plant polysaccharide degradation. Curr Opin Plant Biol. 2008;11(3):338-48. DOI:10.1016/j.pbi.2008.03.004 |
- Eklöf JM and Brumer H. (2010). The XTH gene family: an update on enzyme structure, function, and phylogeny in xyloglucan remodeling. Plant Physiol. 2010;153(2):456-66. DOI:10.1104/pp.110.156844 |
- Schou C, Rasmussen G, Kaltoft MB, Henrissat B, and Schülein M. (1993). Stereochemistry, specificity and kinetics of the hydrolysis of reduced cellodextrins by nine cellulases. Eur J Biochem. 1993;217(3):947-53. DOI:10.1111/j.1432-1033.1993.tb18325.x |
- Sulzenbacher G, Shareck F, Morosoli R, Dupont C, and Davies GJ. (1997). The Streptomyces lividans family 12 endoglucanase: construction of the catalytic cre, expression, and X-ray structure at 1.75 A resolution. Biochemistry. 1997;36(51):16032-9. DOI:10.1021/bi972407v |
- Miao S, Ziser L, Aebersold R, and Withers SG. (1994). Identification of glutamic acid 78 as the active site nucleophile in Bacillus subtilis xylanase using electrospray tandem mass spectrometry. Biochemistry. 1994;33(23):7027-32. DOI:10.1021/bi00189a002 |
- Zechel DL, He S, Dupont C, and Withers SG. (1998). Identification of Glu-120 as the catalytic nucleophile in Streptomyces lividans endoglucanase celB. Biochem J. 1998;336 ( Pt 1)(Pt 1):139-45. DOI:10.1042/bj3360139 |
- Törrönen A, Kubicek CP, and Henrissat B. (1993). Amino acid sequence similarities between low molecular weight endo-1,4-beta-xylanases and family H cellulases revealed by clustering analysis. FEBS Lett. 1993;321(2-3):135-9. DOI:10.1016/0014-5793(93)80094-b |
- Sandgren M, Shaw A, Ropp TH, Wu S, Bott R, Cameron AD, Ståhlberg J, Mitchinson C, and Jones TA. (2001). The X-ray crystal structure of the Trichoderma reesei family 12 endoglucanase 3, Cel12A, at 1.9 A resolution. J Mol Biol. 2001;308(2):295-310. DOI:10.1006/jmbi.2001.4583 |
- Yoshizawa T, Shimizu T, Hirano H, Sato M, and Hashimoto H. (2012). Structural basis for inhibition of xyloglucan-specific endo-β-1,4-glucanase (XEG) by XEG-protein inhibitor. J Biol Chem. 2012;287(22):18710-6. DOI:10.1074/jbc.M112.350520 |
- Sandgren M, Berglund GI, Shaw A, Ståhlberg J, Kenne L, Desmet T, and Mitchinson C. (2004). Crystal complex structures reveal how substrate is bound in the -4 to the +2 binding sites of Humicola grisea Cel12A. J Mol Biol. 2004;342(5):1505-17. DOI:10.1016/j.jmb.2004.07.098 |
- Payan F, Leone P, Porciero S, Furniss C, Tahir T, Williamson G, Durand A, Manzanares P, Gilbert HJ, Juge N, and Roussel A. (2004). The dual nature of the wheat xylanase protein inhibitor XIP-I: structural basis for the inhibition of family 10 and family 11 xylanases. J Biol Chem. 2004;279(34):36029-37. DOI:10.1074/jbc.M404225200 |
- Sandgren M, Gualfetti PJ, Paech C, Paech S, Shaw A, Gross LS, Saldajeno M, Berglund GI, Jones TA, and Mitchinson C. (2003). The Humicola grisea Cel12A enzyme structure at 1.2 A resolution and the impact of its free cysteine residues on thermal stability. Protein Sci. 2003;12(12):2782-93. DOI:10.1110/ps.03220403 |
- Sandgren M, Gualfetti PJ, Shaw A, Gross LS, Saldajeno M, Day AG, Jones TA, and Mitchinson C. (2003). Comparison of family 12 glycoside hydrolases and recruited substitutions important for thermal stability. Protein Sci. 2003;12(4):848-60. DOI:10.1110/ps.0237703 |
- Törrönen A, Harkki A, and Rouvinen J. (1994). Three-dimensional structure of endo-1,4-beta-xylanase II from Trichoderma reesei: two conformational states in the active site. EMBO J. 1994;13(11):2493-501. DOI:10.1002/j.1460-2075.1994.tb06536.x |
-
2007 pmid=17376777
- Kim HW, Kataoka M, and Ishikawa K. (2012). Atomic resolution of the crystal structure of the hyperthermophilic family 12 endocellulase and stabilizing role of the DxDxDG calcium-binding motif in Pyrococcus furiosus. FEBS Lett. 2012;586(7):1009-13. DOI:10.1016/j.febslet.2012.02.029 |
- Cheng YS, Ko TP, Huang JW, Wu TH, Lin CY, Luo W, Li Q, Ma Y, Huang CH, Wang AH, Liu JR, and Guo RT. (2012). Enhanced activity of Thermotoga maritima cellulase 12A by mutating a unique surface loop. Appl Microbiol Biotechnol. 2012;95(3):661-9. DOI:10.1007/s00253-011-3791-4 |
- Kapoor D, Kumar V, Chandrayan SK, Ahmed S, Sharma S, Datt M, Singh B, Karthikeyan S, and Guptasarma P. (2008). Replacement of the active surface of a thermophile protein by that of a homologous mesophile protein through structure-guided 'protein surface grafting'. Biochim Biophys Acta. 2008;1784(11):1771-6. DOI:10.1016/j.bbapap.2008.05.007 |
-
2002 pmid=12204558
- Sulzenbacher G, Mackenzie LF, Wilson KS, Withers SG, Dupont C, and Davies GJ. (1999). The crystal structure of a 2-fluorocellotriosyl complex of the Streptomyces lividans endoglucanase CelB2 at 1.2 A resolution. Biochemistry. 1999;38(15):4826-33. DOI:10.1021/bi982648i |