Glycoside Hydrolase Family 136

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• Author: ^^^Chihaya Yamada^^^
• Responsible Curator: ^^^Shinya Fushinobu^^^

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Glycoside Hydrolase Family GH136
Clan	GH-x
Mechanism	retaining
Active site residues	known
CAZy DB link
https://www.cazy.org/GH136.html

Substrate specificities

This family of glycoside hydrolases contains lacto-N-biosidase, as demonstrated for LnbX from Bifidobacterium longum JCM 1217 [1]. LnbX liberates the disaccharide Galβ1-3GlcNAc(lacto-N-biose I, LNB) from Human Milk Oligossacharides, lacto-N-tetraose, as substrate of the GH136 lacto-N-biosidase from B. longum JCM 1217. In addition, LnbX liberates Galβ1-3GalNAc (GNB) from the sugar chains of globo- and ganglio-series glycosphingolipids [2].

Kinetics and Mechanism

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Catalytic Residues

The nucleophile is Asp418. The catalytic acid/base is Asp411 via water molecule.

Three-dimensional structures

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Family Firsts

First stereochemistry determination

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First catalytic nucleophile identification

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First general acid/base residue identification

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First 3-D structure

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References

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