Talk:Glycoside Hydrolase Family 3

Litzinger et al. (2010) suggested that the acid/base catalyst of 2 domain NagZs (and also 1 domain NagZs) is an Asp-His dyad in the (β/α)₈ domain. pmid: 20826810.

Update to Catalytic Residues section needed?

Given the growing amount of information about the identity of the GH3 catalytic residues, and in particular the acid/base, in different enzymes/subfamilies, I wonder if a update of this section isn't needed?

Here are some key acid/base ID references [1, 2, 3, 4, 5, 6, 7, 8, 9], one of which also confirms the catalytic nucleophile [7]. These references are nucleophile-specific studies [10, 11]:

References

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1. Subfamily 4, via kinetic analysis & azide rescue

2. NagZ subfamily, ligand complex

3. NagZ sunfamily, kinetic analysis

4. Subfamily 5

5. Subfamily 5

6. double-check

7. Subfamily 5

8. Subfamily 5

9. Nag - relevant? Mis-identification.

Harry Brumer (talk) 17:55, 14 December 2012 (PST)