User:Wataru Saburi

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Wataru Saburi is an assistant professor at Laboratory of Biochemistry in Research Faculty of Agriculture, Hokkaido University (Sapporo, Japan). He obtained Ph. D from Graduate School of Agriculture, Hokkaido University in 2006, under the supervision of Professor Atsuo Kimura. He joined the Research Institute of Nihon Shokuhin Kako Co. Ltd. as a researcher (2006-2010), and developed functional oligosaccharides produced from starch. His research interests are structures and functions of carbohydrate active enzymes and efficient synthesis of functional oligosaccharides. He has studied about

• GH1 rice β-glucosidase [1]
• GH13 Bacillus sp. AAH-31 α-amylase [2, 3]
• GH13 Streptococcus mutans dextran glucosidase [4, 5, 6, 7, 8]
• GH13 Halomonas sp. H11 α-glucosidase [9]
• GH13 Bacillus clarkii γ-cyclodextrinase [10]

• GH31 Bacillus sp. AHU 2001 α-glucosidase BspAG31A [11]
• GH94 Ruminococcus albus cellobiose phosphorylase [12, 13]
• GH94 Ruminococcus albus cellodextrin phosphorylase [14]
• GH130 Ruminococcus albus 4-O-β-mannosylglucose phosphorylase (RaMP1) [15]
• GH130 Rhodothermus marinus 4-O-β-mannosylglucose phosphorylase [16]
• GH130 Ruminococcus albus β-1,4-mannooligosaccharide phosphorylase (RaMP2) [15]

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6. Hondoh H, Saburi W, Mori H, Okuyama M, Nakada T, Matsuura Y, and Kimura A. (2008). Substrate recognition mechanism of alpha-1,6-glucosidic linkage hydrolyzing enzyme, dextran glucosidase from Streptococcus mutans. J Mol Biol. 2008;378(4):913-22. DOI:10.1016/j.jmb.2008.03.016 |
15. Kawahara R, Saburi W, Odaka R, Taguchi H, Ito S, Mori H, and Matsui H. (2012). Metabolic mechanism of mannan in a ruminal bacterium, Ruminococcus albus, involving two mannoside phosphorylases and cellobiose 2-epimerase: discovery of a new carbohydrate phosphorylase, β-1,4-mannooligosaccharide phosphorylase. J Biol Chem. 2012;287(50):42389-99. DOI:10.1074/jbc.M112.390336 |