Glycoside Hydrolase Family 143

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Glycoside Hydrolase Family GH143
Clan	GH-x
Mechanism	retaining/inverting
Active site residues	known/not known
CAZy DB link
https://www.cazy.org/GH143.html

Substrate specificities

The members of GH142 family are 1-keto-3-deoxy-D-lyxo-heptulosaric acid (DHA) hydrolases (EC 3.2.1.-). The first characterized enzyme from this family was the N-terminus of BT1020 from Bacteroides thetaiotaomicron [1]. BT1020 cleaves the D-DHA- β-2,3-D-GalA linkage in rhamnogalacturonan II (RG II) in pectin.

Kinetics and Mechanism

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Catalytic Residues

The active site of DHA-hydrolase (N-terminus of BT1020) contains tyrosine and glutamate residues that function as the catalytic nucleophile and acid-base residues [1].

Three-dimensional structures

The N-terminus of BT1020 has a 5-bladed β-propeller structure with 2-keto-3-deoxy-D-lyxo-heptulosaric acid (DHA)-hydrolase activity [1].

Family Firsts

First stereochemistry determination: Not yet identified.
First catalytic nucleophile identification: BT1020 from Bacteroides thetaiotaomicron [1].
First general acid/base residue identification: BT1020 from Bacteroides thetaiotaomicron [1].
First 3-D structure: BT1020 from Bacteroides thetaiotaomicron [1].

References

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Error fetching PMID 28329766: [Ndeh2017]