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Difference between revisions of "Glycoside Hydrolase Family 12"
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== Substrate specificities == | == Substrate specificities == | ||
The substrate specificities found among the [[glycoside hydrolases]] of family 12 are: ''[[endo]]''-β-1,4-glucanase (EC 3.2.1.4), ''xyloglucan hydrolase (EC 3.2.1.151), ''[[endo]]''-β-1,3-1,4-glucanase (EC 3.2.1.73), and xyloglucan ''[[endo]]''-transglycosylase (EC 2.4.1.207),. | The substrate specificities found among the [[glycoside hydrolases]] of family 12 are: ''[[endo]]''-β-1,4-glucanase (EC 3.2.1.4), ''xyloglucan hydrolase (EC 3.2.1.151), ''[[endo]]''-β-1,3-1,4-glucanase (EC 3.2.1.73), and xyloglucan ''[[endo]]''-transglycosylase (EC 2.4.1.207),. | ||
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== Kinetics and Mechanism == | == Kinetics and Mechanism == | ||
| − | + | GH Family 12 enzymes are [[retaining]] enzymes, as first shown by NMR studdies <cite>Schou1993</cite> of endoglucanase 3 from ''Humicola insolens'', and follow a classical [[Koshland double-displacement mechanism]]. No detailed studies involving both steady state and pre-steady state kinetic have jet been reported for this family. | |
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== Catalytic Residues == | == Catalytic Residues == | ||
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== References == | == References == | ||
<biblio> | <biblio> | ||
| − | # | + | #Schou1993 pmid=8223652 |
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</biblio> | </biblio> | ||
[[Category:Glycoside Hydrolase Families|GH012]] | [[Category:Glycoside Hydrolase Families|GH012]] | ||
Revision as of 12:26, 5 April 2010
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
- Author: ^^^Mats Sandgren^^^
- Responsible Curator: ^^^Mats Sandgren^^^
| Glycoside Hydrolase Family GHnn | |
| Clan | GH-C |
| Mechanism | retaining |
| Active site residues | known |
| CAZy DB link | |
| http://www.cazy.org/fam/GH12.html | |
Substrate specificities
The substrate specificities found among the glycoside hydrolases of family 12 are: endo-β-1,4-glucanase (EC 3.2.1.4), xyloglucan hydrolase (EC 3.2.1.151), endo-β-1,3-1,4-glucanase (EC 3.2.1.73), and xyloglucan endo-transglycosylase (EC 2.4.1.207),.
Kinetics and Mechanism
GH Family 12 enzymes are retaining enzymes, as first shown by NMR studdies [1] of endoglucanase 3 from Humicola insolens, and follow a classical Koshland double-displacement mechanism. No detailed studies involving both steady state and pre-steady state kinetic have jet been reported for this family.
Catalytic Residues
Content is to be added here.
Three-dimensional structures
Content is to be added here.
Family Firsts
- First sterochemistry determination
- Cite some reference here, with a short (1-2 sentence) explanation [2].
- First catalytic nucleophile identification
- Cite some reference here, with a short (1-2 sentence) explanation [3].
- First general acid/base residue identification
- Cite some reference here, with a short (1-2 sentence) explanation [4].
- First 3-D structure
- Cite some reference here, with a short (1-2 sentence) explanation [5].