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Difference between revisions of "Glycoside Hydrolase Family 147"

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Revision as of 11:51, 15 February 2018

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Glycoside Hydrolase Family GH147
Clan GH-A
Mechanism retaining
Active site residues known
CAZy DB link
http://www.cazy.org/GH147.html


Substrate specificities

The founding member of GH147, BACOVA_05493 from Bacteroides ovatus is a β1,4-galactosidase. The enzyme demonstrates a preference towards longer oligosaccharides and β1,4-galactan, releasing galactopyranose from the oligosaccharide or polysaccharide chain. BACOVA_05493 is unable to hydrolyse galactobiose [1].

Kinetics and Mechanism

NMR analysis of the galactose product released from galactotriose revealed a retaining mechanism of action [1].

Catalytic Residues

Hydrophobic cluster analysis showed that BACOVA_05493 is a member of Clan GH-A. Based on this assumption the catalytic nucleophile and general acid/base residues of the founding member of GH147 were proposed as Glu300 and Glu203, respectively. This hypothesis was supported by mutagenesis data showing that the E300A and E203A mutants were catalytically inactive [1].

Three-dimensional structures

Currently there is no crystal structure of any member of GH147, although sequence analysis and hydrophobic cluster analysis predicts a fold of an (α/β)8 barrel.

Family Firsts

First stereochemistry determination
BACOVA_05493 from B. ovatus was shown to have a retaining mechanism [1].
First catalytic nucleophile identification
BACOVA_05493 from B. ovatus [1].
First general acid/base residue identification
BACOVA_05493 from B. ovatus [1].
First 3-D structure
Currently no experimental structure is available, although BACOVA_05493 is predicted to fold into a (α/β)8 barrel [1].

References

  1. Luis AS, Briggs J, Zhang X, Farnell B, Ndeh D, Labourel A, Baslé A, Cartmell A, Terrapon N, Stott K, Lowe EC, McLean R, Shearer K, Schückel J, Venditto I, Ralet MC, Henrissat B, Martens EC, Mosimann SC, Abbott DW, and Gilbert HJ. (2018) Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 3, 210-219. DOI:10.1038/s41564-017-0079-1 | PubMed ID:29255254 | HubMed [Luis2017]