Glycoside Hydrolase Family 29

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• Author: ^^^Gerlind Suzlenbacher^^^
• Responsible Curator: ^^^Steve Withers^^^

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Glycoside Hydrolase Family GH 29
Clan	none
Mechanism	retaining
Active site residues	known
CAZy DB link
http://www.cazy.org/fam/GH29.html

Substrate specificities

The glycoside hydrolases of this family are exo-acting α-fucosidases from archaeal, bacterial and eukaryotic origin. No other activities have been observed for GH29 family members. So fare the only other CAZY family containing α-fucosidases is family GH95. The human enzyme FucA1 is of medical interest because its deficiency leads to fucosidosis, an autosomal recessive lysosomal storage disease [1].

Kinetics and Mechanism

GH29 α-fucosidases are retaining enzymes following a classical Koshland double-displacement mechanism, as first proposed in 1987 for human liver α-fucosidase via burst kinetics experiments and using methanol as an alternative glycone acceptor to produce methyl-α-L-fucoside [2].(White et al, Biochim. Biophys. Acta 912, 132–138, PMID: 3828350). This has been further confirmed by 1H NMR monitoring of the reaction catalyzed by α-L-fucosidase from Thermus sp. (Eneyskaya et al. Glycoconj. J. 18, 827–834 PMID: 12441672) and α-L-fucosidase from the marine mollusc Pecten maximus (Berteau et al., Glycobiology 12, 273–282 PMID: 12042250), as well as by COSY and 1H-13C NMR spectroscopy analysis of the interglycosidic linkage of disaccharides formed by the transglycosylation action of Sulfolobus solfataricus α-L-fucosidase (PMID: 12569098). GH95 α-fucosidases, in contrast, operate with inversion of the anomeric configuration.

Catalytic Residues

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Three-dimensional structures

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Family Firsts

First sterochemistry determination

Cite some reference here, with a short (1-2 sentence) explanation [3].

First catalytic nucleophile identification

Cite some reference here, with a short (1-2 sentence) explanation [4].

First general acid/base residue identification

Cite some reference here, with a short (1-2 sentence) explanation [5].

First 3-D structure

Cite some reference here, with a short (1-2 sentence) explanation [6].

References

1. O'Brien JS, Willems PJ, Fukushima H, de Wet JR, Darby JK, Di Cioccio R, Fowler ML, and Shows TB. (1987). Molecular biology of the alpha-L-fucosidase gene and fucosidosis. Enzyme. 1987;38(1-4):45-53. DOI:10.1159/000469189 |
2. White WJ Jr, Schray KJ, Legler G, and Alhadeff JA. (1987). Further studies on the catalytic mechanism of human liver alpha-L-fucosidase. Biochim Biophys Acta. 1987;912(1):132-8. DOI:10.1016/0167-4838(87)90256-1 |

[[Category:Glycoside Hydrolase Families|GHnnn]]