CAZypedia needs your help! We have many unassigned GH, PL, CE, AA, GT, and CBM pages in need of Authors and Responsible Curators.
Scientists at all career stages, including students, are welcome to contribute to CAZypedia. Read more here, and in the 10th anniversary article in Glycobiology.
New to the CAZy classification? Read this first.
*
Consider attending the 15th Carbohydrate Bioengineering Meeting in Ghent, 5-8 May 2024.
Glycoside Hydrolase Family 54
Glycoside Hydrolase Family 54 | |
Clan | none |
Mechanism | retaining |
Active site residues | known |
CAZy DB link | |
http://www.cazy.org/fam/GH54.html |
Substrate specificities
This family contains α-L-arabinofuranosidase (EC 3.2.1.55) and β-xylosidase (EC 3.2.1.37).
Kinetics and Mechanism
GH54 members are retaining enzymes.
Catalytic Residues
Three-dimensional structures
The first solved 3-D structure was α-L-arabinofuranosidase B (AkAbfB) from Aspergillus kawachii IFO 4308 (http://www.rcsb.org/pdb/explore/explore.do?structureId=1WD3 PDB 1wd3) in 2004 [1].
Carbohydrate-Binding Module
Some members in GH54 has Carbohydrate-Binding Module Family 42 at their C-terminus.
Family Firsts
- First sterochemistry determination
- Cite some reference here, with a short explanation.
- First catalytic nucleophile identification
- First general acid/base residue identification
- First 3-D structure
- α-L-Arabinofuranosidase B (AkAbfB) from Aspergillus kawachii IFO 4308 (http://www.rcsb.org/pdb/explore/explore.do?structureId=1WD3 PDB 1wd3) [1].