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Glycoside Hydrolase Family 6
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- Author: ^^^Kathleen Piens^^^ and ^^^Gideon Davies^^^
- Responsible Curator: ^^^Gideon Davies^^^
Glycoside Hydrolase Family GH6 | |
Clan | none |
Mechanism | inverting |
Active site residues | acid known, base debated |
CAZy DB link | |
http://www.cazy.org/fam/GH6.html |
Substrate specificities
Endoglucanases (EC 3.2.1.4) and cellobiohydrolases (EC 3.2.1.91)only. Both classes of enzyme active on cellulose / beta 1,4 glucans.
Kinetics and Mechanism
Content is to be added here.
Catalytic Residues
Content is to be added here.
Proposed role | CfCel6A (endo) | HiCel6A (exo) | HjCel6A (exo) | TfCel6A (endo) | TfCel6B (exo) |
---|---|---|---|---|---|
Substrate distortion | Asp210 | Tyr174 | Tyr169 | Tyr73 | Tyr220 |
Increase in pKa acid/Catalytic base | Asp216 | Asp180 | Asp175 | Asp79 | Asp226 |
Proton network | Gly222 | Ser186 | Ser181 | Ser85 | Asp232 |
Catalytic acid | Asp252 | Asp226 | Asp221 | Asp117 | Asp274 |
Catalytic base/substrate binding | Asp392 | Asp405 | Asp401 | Asp265 | Asp497 |
Three-dimensional structures
Content is to be added here.
Family Firsts
- First sterochemistry determination
- Cite some reference here, with a short (1-2 senetence) explanation [1].
- First general acid/base residue identification
- Cite some reference here, with a short (1-2 senetence) explanation [2].
- First 3-D structure
- The catalytic core domain of the Trichoderma reesei (the organism now known as Hypocrea jecorina) cellobiohydrolase II by the Jones group [3]. The first endoglucanase in this family was the Thermomonospora fusca E2 enzyme (catalytic core) solved by the Wilson/Karplus groups[4]
References
- Rouvinen J, Bergfors T, Teeri T, Knowles JK, and Jones TA. (1990). Three-dimensional structure of cellobiohydrolase II from Trichoderma reesei. Science. 1990;249(4967):380-6. DOI:10.1126/science.2377893 |
- Spezio M, Wilson DB, and Karplus PA. (1993). Crystal structure of the catalytic domain of a thermophilic endocellulase. Biochemistry. 1993;32(38):9906-16. DOI:10.1021/bi00089a006 |
- Robert V. Stick and Spencer J. Williams. (2009) Carbohydrates. Elsevier Science.
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Sinnott, M.L. (1990) Catalytic mechanisms of enzymic glycosyl transfer. Chem. Rev. 90, 1171-1202. DOI: 10.1021/cr00105a006