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Difference between revisions of "Glycoside Hydrolase Family 62"
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== Substrate specificities == | == Substrate specificities == | ||
| − | + | Arabinofuranosidases that specifically cleave either alpha1,2 or alpha1,3 L-arabinofuranose side chains from xylans <cite>#1 #2</cite>. The enzyme will not act on xylose moieties in xylan that are decorated at both O2 and O3 with an arabinose side chain. | |
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== Kinetics and Mechanism == | == Kinetics and Mechanism == | ||
Revision as of 14:50, 28 July 2009
| Glycoside Hydrolase Family GH62 | |
| Clan | GH-F |
| Mechanism | assumed to be inverting |
| Active site residues | inferred |
| CAZy DB link | |
| http://www.cazy.org/fam/GH62.html | |
Substrate specificities
Arabinofuranosidases that specifically cleave either alpha1,2 or alpha1,3 L-arabinofuranose side chains from xylans [1, 2]. The enzyme will not act on xylose moieties in xylan that are decorated at both O2 and O3 with an arabinose side chain.
Kinetics and Mechanism
Catalytic Residues
Three-dimensional structures
Family Firsts
- First sterochemistry determination
- Cite some reference here, with a short explanation [1].
- First catalytic nucleophile identification
- First general acid/base residue identification
- First 3-D structure
""
References
- Comfort DA, Bobrov KS, Ivanen DR, Shabalin KA, Harris JM, Kulminskaya AA, Brumer H, and Kelly RM. (2007). Biochemical analysis of Thermotoga maritima GH36 alpha-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D glycoside hydrolases. Biochemistry. 2007;46(11):3319-30. DOI:10.1021/bi061521n |
[[Category:Glycoside Hydrolase Families]]