Glycoside Hydrolase Family 86

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• Author: ^^^Mirjam Czjzek^^^
• Responsible Curator: ^^^Mirjam Czjzek^^^

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Glycoside Hydrolase Family GH86
Clan	GH-A
Mechanism	probably retaining
Active site residues	inferred from clan GH-A as two Glu
CAZy DB link
http://www.cazy.org/GH86.html

Substrate specificities

To date, all characterized glycoside hydrolases of family 86 are β-agarases (EC 3.2.1.81) that cleave β-1,4 glycosidic bonds of agarose. To date three enzymes have been characterized: AgrA from Pseudoalteromonas atlantica, AgaO from Microbulbifer thermotolerans JAMB-A94 and Aga86E from Saccharophagus degradans 2-40 [1, 2, 3]. AgaO from M. thermotolerans was reported to be an endo-hydrolytic enzyme, releasing neoagaro-hexaose as main product [2], while the recombinant Aga86E from S. degradans released only neoagarobiose in an exo-acting manner.

Kinetics and Mechanism

Actually, a potential retaining mechanism of this glycoside hydrolase family can only be inferred from analogy to clan GH-A enzymes http://www.cazy.org/GH86.html. No mechanistic or kintetic analysis demonstrating the stereochemical outcome of the reaction have been reported for this family to date.

Catalytic Residues

Actually, the catalytic residues can only be inferred from analogy to clan GH-A enzymes as two glutamate residues.

Three-dimensional structures

No 3D structure is available to date.

Family Firsts

First stereochemistry determination

Cite some reference here, with a short (1-2 sentence) explanation [4].

First catalytic nucleophile identification

Cite some reference here, with a short (1-2 sentence) explanation [5].

First general acid/base residue identification

Cite some reference here, with a short (1-2 sentence) explanation [6].

First 3-D structure

Cite some reference here, with a short (1-2 sentence) explanation [7].

References

1. Belas R (1989). Sequence analysis of the agrA gene encoding beta-agarase from Pseudomonas atlantica. J Bacteriol. 1989;171(1):602-5. DOI:10.1128/jb.171.1.602-605.1989 |
2. Ohta Y, Hatada Y, Nogi Y, Li Z, Ito S, and Horikoshi K. (2004). Cloning, expression, and characterization of a glycoside hydrolase family 86 beta-agarase from a deep-sea Microbulbifer-like isolate. Appl Microbiol Biotechnol. 2004;66(3):266-75. DOI:10.1007/s00253-004-1757-5 |
3. Ekborg NA, Taylor LE, Longmire AG, Henrissat B, Weiner RM, and Hutcheson SW. (2006). Genomic and proteomic analyses of the agarolytic system expressed by Saccharophagus degradans 2-40. Appl Environ Microbiol. 2006;72(5):3396-405. DOI:10.1128/AEM.72.5.3396-3405.2006 |