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Difference between revisions of "Polysaccharide Lyase Family 8"
| Line 30: | Line 30: | ||
== Known Activities == | == Known Activities == | ||
| − | hyaluronate lyase (EC 4.2.2.1); chondroitin AC lyase (EC 4.2.2.5); xanthan lyase (EC 4.2.2.12); chondroitin ABC lyase (EC 4.2.2.20). | + | PL8s display a broad range of enzymatic activities including: hyaluronate lyase (EC 4.2.2.1); chondroitin AC lyase (EC 4.2.2.5); xanthan lyase (EC 4.2.2.12); and chondroitin ABC lyase (EC 4.2.2.20). |
== Substrate specificities == | == Substrate specificities == | ||
| − | + | PL8s are active on a variety of uronic acid-containing polysaccharides including '''hyaluronan''' [4)-β-D-Glucuronate-1,3-β -D-N-Acetyl-Glucosamine(1]<sub>n</sub>, '''chondroitin AC''' [4)-β-D-Glucuronate-1,3-β-D-N-Acetyl-GalactosamineΔ4,6S(1]<sub>n</sub>, '''xanthan''' [4)-β-D-Glucuronate-1,4-β-D-Glucuronate (1]<sub>n</sub>, and '''chondroitin ABC''' [chondroitin AC and chondroitin B (aka. dermatan sulfate: 4)-β-L-Iduronate2S-1,3-β-D-N-Acetyl-Galactosamine4S(1]<sub>n</sub>. | |
== Kinetics and Mechanism == | == Kinetics and Mechanism == | ||
| Line 53: | Line 53: | ||
;First general acid/base residue identification: Content is to be added here. | ;First general acid/base residue identification: Content is to be added here. | ||
;First 3-D structure: Chondroitin AC lyase – “Bacteroides stercoris” HJ-15 <cite>Fethiere1999</cite>([{{PDBlink}}1CB8 PDB 1CB8]). | ;First 3-D structure: Chondroitin AC lyase – “Bacteroides stercoris” HJ-15 <cite>Fethiere1999</cite>([{{PDBlink}}1CB8 PDB 1CB8]). | ||
| + | First Structure by Activity: | ||
| + | Hyaluronidase – “Streptococcus pneumoniae” R6 <cite>Li2000</cite>([{{PDBlink}}1OJM PDB 1OJM]). | ||
| + | Chondroitin AC lyase – “Bacteroides stercoris” HJ-15 <cite>Fethiere1999</cite>([{{PDBlink}}1CB8 PDB 1CB8]). | ||
| + | Xanthanase – “Bacillus” sp GL1 <cite>Hashimoto2003</cite>([{{PDBlink}}1J0M PDB 1J0M]). | ||
| + | Chondroitin ABC lyase – “Proteus vulgaris” <cite>Huang2003</cite>([{{PDBlink}}1HN0 PDB 1HN0]). | ||
== References == | == References == | ||
Revision as of 11:34, 6 November 2013
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
- Author: ^^^Michael Suits^^^
- Responsible Curator: ^^^Michael Suits^^^
| Polysaccharide Lyase Family PL8 | |
| 3D Structure | (α/α)6 barrel + anti-parallel β-sheet |
| Mechanism | β-elimination |
| Active site residues | known/not known |
| CAZy DB link | |
| http://www.cazy.org/PL8.html | |
Known Activities
PL8s display a broad range of enzymatic activities including: hyaluronate lyase (EC 4.2.2.1); chondroitin AC lyase (EC 4.2.2.5); xanthan lyase (EC 4.2.2.12); and chondroitin ABC lyase (EC 4.2.2.20).
Substrate specificities
PL8s are active on a variety of uronic acid-containing polysaccharides including hyaluronan [4)-β-D-Glucuronate-1,3-β -D-N-Acetyl-Glucosamine(1]n, chondroitin AC [4)-β-D-Glucuronate-1,3-β-D-N-Acetyl-GalactosamineΔ4,6S(1]n, xanthan [4)-β-D-Glucuronate-1,4-β-D-Glucuronate (1]n, and chondroitin ABC [chondroitin AC and chondroitin B (aka. dermatan sulfate: 4)-β-L-Iduronate2S-1,3-β-D-N-Acetyl-Galactosamine4S(1]n.
Kinetics and Mechanism
Content is to be added here.
Catalytic Residues
Content is to be added here.
Three-dimensional structures
Structure by Activity: Hyaluronidase – “Streptococcus pneumoniae” R6 [1](PDB 1OJM). Chondroitin AC lyase – “Bacteroides stercoris” HJ-15 [2](PDB 1CB8). Xanthanase – “Bacillus” sp GL1 [3](PDB 1J0M). Chondroitin ABC lyase – “Proteus vulgaris” [4](PDB 1HN0).
Family Firsts
- First stereochemistry determination
- Content is to be added here.
- First catalytic nucleophile identification
- Content is to be added here.
- First general acid/base residue identification
- Content is to be added here.
- First 3-D structure
- Chondroitin AC lyase – “Bacteroides stercoris” HJ-15 [2](PDB 1CB8).
First Structure by Activity: Hyaluronidase – “Streptococcus pneumoniae” R6 [1](PDB 1OJM). Chondroitin AC lyase – “Bacteroides stercoris” HJ-15 [2](PDB 1CB8). Xanthanase – “Bacillus” sp GL1 [3](PDB 1J0M). Chondroitin ABC lyase – “Proteus vulgaris” [4](PDB 1HN0).
References
<biblio>
- Rapport1951 pmid=14917676
- Li2000 pmid=10716923
- Fethiere1999 pmid=10329169
- Sato1994 pmid=7512814
- Huang2003 pmid=12706721
- Hashimoto1998 pmid=9758797
- Hashimoto2003 pmid=16348550