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Glycoside Hydrolase Family 121

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Glycoside Hydrolase Family GH121
Clan none
Mechanism retaining
Active site residues not known
CAZy DB link
http://www.cazy.org/GH121.html

Substrate specificities

This family of glycoside hydrolases contains β-L-arabinobiosidases, as demonstrated for HypBA2 from Bifidobacterium longum JCM 1217 [1]. HypBA2 liberates the disaccharide Arafβ1-2Araf (β-Ara2, a substrate of the GH127 β-L-arabinofuranosidase from B. longum JCM 1217 [2]) from unmodified Arafβ1-2Arafβ1-2Arafβ-hydroxyproline (Ara3-Hyp), but not Arafα1-3Arafβ1-2Arafβ1-2Arafβ-Hyp (Ara4-Hyp) or Arafβ1-2Arafβ-Hyp (Ara2-Hyp). HypBA2 directly liberates β-Ara2 from hydroxyproline-rich glycoproteins (HRGPs) such as carrot extensin and potato lectin. The family members are only found from prokaryote genomes, such as bacteria and actinomycetes.

Kinetics and Mechanism

HypBA2 is a retaining enzyme. The stereochemical course of the reaction was shown by transglycosylation activity toward 1-alkanols, such as methanol; the resulting Arafβ1-2Arafβ-Me was identified by 1H-NMR and 13C-NMR analysis [1].

Catalytic Residues

The catalytic residues are not known but three conserved residues (Glu373, Asp515, and Glu713 in B. longum HypBA2) are the candidates based on mutagenesis and structural comparison [3].

Three-dimensional structures

Figure 1: β-L-arabinobiosidase HypBA2 from Bifidobacterium longum. The catalytic (α/α)6 barrel domain is in green.

The first solved 3-D structure was β-L-arabinobiosidase HypBA2 from Bifidobacterium longum (PDB 6M5A) [3]. The catalytic domain adops an (α/α)6 barrel fold similar to GH142, GH63, GH78, GH94, and GH37 (Figure 1).

Family Firsts

First stereochemistry determination
Shown to be retaining for HypBA2 by product analysis of glycosyl transfer reactions to methanol [1].
First catalytic nucleophile identification
Predicted based on structural homology [3], but currently no experimental proof.
First general acid/base residue identification
Predicted based on structural homology [3], but currently no experimental proof.
First 3-D structure
β-L-arabinobiosidase HypBA2 from Bifidobacterium longum [3].

References

Error fetching PMID 21149454:
Error fetching PMID 21914802:
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  1. Error fetching PMID 21149454: [Fujita2011A]
  2. Error fetching PMID 21914802: [Fujita2011B]
  3. Error fetching PMID 32479540: [Saito2020]
All Medline abstracts: PubMed | HubMed