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Difference between revisions of "User:Takayuki Ohnuma"

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[[File:Taka.jpg|right|150px|caption]]Takayuki Ohnuma is an assistant professor at Department of Advanced Bioscience, Faculty of Agriculture, Kinki University located in Nara, Japan.
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[[File:Taka.jpg|200px|right]]
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He received PhD degree in 2002 from Graduate School of Agriculture in Kyushu University. His research program currently focuses on the structures and functions of plant chitinases (GH18 and GH19), including their CBMs (CBM18 and CBM50), which are involved in plant self-defense against fungal pathogens [1-11]. In 2013, He and his colabolater published [2] the structure of a complex of rye family 19 chitinase with two molecules of (GlcNAc)<sub>4</sub>. This is the first report on the complete subsite mapping of GH19 chitinase.
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Takayuki Ohnuma is an assistant professor at Department of Advanced Bioscience, Faculty of Agriculture, Kinki University located in Nara, Japan.
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He received his Ph.D. degree in 2002 from the Graduate School of Agriculture in Kyushu University. His research program currently focuses on the structures and functions of chitinolytic enzymes <cite>Ohnuma2011a Ohnuma2011b Ohnuma2011c Ohnuma2012 Ohnuma2013 Ohnuma2014</cite>, including their CBMs <cite>Ohnuma2008 Shinya2013</cite>. In 2013, He and his collaborators published the structure of a complex of rye [[Glycoside Hydrolase Family 19]] chitinase with two molecules of (GlcNAc)<sub>4</sub> <cite>Ohnuma2013</cite>. This is the first report on the complete subsite mapping of a [[GH19]] chitinase.
  
  
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References
 
References
  
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# Ohnuma T, Umemoto N, Nagata T, Shinya S, Numata T, Taira T, Fukamizo T. Crystal structure of a "loopless" GH19 chitinase in complex with chitin tetrasaccharide spanning the catalytic center. ''Biochim Biophys Acta'', 1844, 793-802 (2014)
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<biblio>
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# Ohnuma T, Umemoto N, Kondo K, Numata T, Fukamizo T. Complete subsite mapping of a "loopful" GH19 chitinase from rye seeds based on its crystal structure. ''FEBS Lett'', 587, 2691-7 (2013)
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#Ohnuma2011a pmid=21240541
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# Ohnuma T, Taira T, Fukamizo T. Antifungal activity of recombinant class V chitinases from ''Nicotiana tabacum'' and ''Arabidopsis thaliana''. ''J Appl Glycosci'', 59, 1, 47-50 (2012)
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#Ohnuma2011b pmid=21390509
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# Umemoto N, Ohnuma T, Mizuhara M, Sato H, Skriver K, Fukamizo T. Introduction of a tryptophan side chain into subsite +1 enhances transglycosylation activity of a GH-18 chitinase from ''Arabidopsis thaliana'', AtChiC. ''Glycobiology'', 23, 81-90 (2013)
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#Ohnuma2011c pmid=21838762
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# Ohnuma T, Numata T, Osawa T, Inanaga H, Okazaki Y, Shinya S, Kondo K, Fukuda T, Fukamizo T. Crystal structure and chitin oligosaccharide-binding mode of a 'loopful' family GH19 chitinase from rye, ''Secale cereale'', seeds. ''FEBS J'', 19, 3639-3651 (2012)
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#Ohnuma2012 pmid=22831795
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# Umemoto N, Ohnuma T, Urpilainen H, Yamamoto T, Numata T, Fukamizo T. Role of tryptophan residues in a class V chitinase from ''Nicotiana tabacum''. ''Biosci Biotechnol Biochem'', 76,778-784 (2012)
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#Ohnuma2013 pmid=23871710
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# Ohnuma T, Fukuda T, Dozen S, Honda Y, Kitaoka M, Fukamizo T. A glycosynthase derived from an inverting GH19 chitinase from the moss ''Bryum coronatum''. ''Biochem J'', 444, 437-443 (2012)
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#Ohnuma2014 pmid=24582745
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# Shinya S, Ohnuma T, Kawamura S, Torikata T, Nishimura S, Katoh E, Fukamizo T. Interaction of a goose-type lysozyme with chitin oligosaccharides as determined by NMR spectroscopy. ''J Biochem'', 150, 569-577 (2011)
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#Ohnuma2008 pmid=18083709
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# Ohnuma T, Sørlie M, Fukuda T, Kawamoto N, Taira T, Fukamizo T. Chitin oligosaccharide binding to a family GH19 chitinase from the moss ''Bryum coronatum''. ''FEBS J'', 278, 3991-4001 (2011)
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#Shinya2013 pmid=23986450
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# Ohnuma T, Numata T, Osawa T, Mizuhara M, Lampela O, Juffer AH, Skriver K, Fukamizo T. A class V chitinase from ''Arabidopsis thaliana'': gene responses, enzymatic properties, and crystallographic analysis. ''Planta'', 234, 123-137 (2011)
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</biblio>
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# Ohnuma T, Numata T, Osawa T, Mizuhara M, Vårum KM, Fukamizo T. Crystal structure and mode of action of a class V chitinase from ''Nicotiana tabacum''. ''Plant Mol Biol'', 75, 291-304 (2011)                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
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[[Category:Contributors|Ohnuma, Takayuki]]
 
[[Category:Contributors|Ohnuma, Takayuki]]

Latest revision as of 09:15, 8 December 2016

Taka.jpg

Takayuki Ohnuma is an assistant professor at Department of Advanced Bioscience, Faculty of Agriculture, Kinki University located in Nara, Japan. He received his Ph.D. degree in 2002 from the Graduate School of Agriculture in Kyushu University. His research program currently focuses on the structures and functions of chitinolytic enzymes [1, 2, 3, 4, 5, 6], including their CBMs [7, 8]. In 2013, He and his collaborators published the structure of a complex of rye Glycoside Hydrolase Family 19 chitinase with two molecules of (GlcNAc)4 [5]. This is the first report on the complete subsite mapping of a GH19 chitinase.


References

Error fetching PMID 21240541:
Error fetching PMID 21390509:
Error fetching PMID 21838762:
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Error fetching PMID 18083709:
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  1. Error fetching PMID 21240541: [Ohnuma2011a]
  2. Error fetching PMID 21390509: [Ohnuma2011b]
  3. Error fetching PMID 21838762: [Ohnuma2011c]
  4. Error fetching PMID 22831795: [Ohnuma2012]
  5. Error fetching PMID 23871710: [Ohnuma2013]
  6. Error fetching PMID 24582745: [Ohnuma2014]
  7. Error fetching PMID 18083709: [Ohnuma2008]
  8. Error fetching PMID 23986450: [Shinya2013]

All Medline abstracts: PubMed

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