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Difference between revisions of "Glycoside Hydrolase Family 121"

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* [[Author]]: [[User:Kiyotaka Fujita|Kiyotaka Fujita]]
* [[Author]]: ^^^Kiyotaka Fujita^^^
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* [[Responsible Curator]]:  [[User:Shinya Fushinobu|Shinya Fushinobu]]
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== Substrate specificities ==
 
== Substrate specificities ==
This family of [[glycoside hydrolases]] contains &beta;-L-arabinobiosidase, which was recently established for HypBA2 from ''Bifidobacterium longum'' JCM 1217<cite>Fujita2011A</cite>.  
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This family of [[glycoside hydrolases]] contains &beta;-L-arabinobiosidases, as demonstrated for HypBA2 from ''Bifidobacterium longum'' JCM 1217 <cite>Fujita2011A</cite>. HypBA2 liberates the disaccharide Ara''f''&beta;1-2Ara''f'' (&beta;-Ara<sub>2</sub>, a substrate of the [[GH127]] &beta;-L-arabinofuranosidase from ''B. longum'' JCM 1217 <cite>Fujita2011B</cite>) from unmodified Ara''f''&beta;1-2Ara''f''&beta;1-2Ara''f''&beta;-hydroxyproline (Ara<sub>3</sub>-Hyp), but not Ara''f''&alpha;1-3Ara''f''&beta;1-2Ara''f''&beta;1-2Ara''f''&beta;-Hyp (Ara<sub>4</sub>-Hyp) or Ara''f''&beta;1-2Ara''f''&beta;-Hyp (Ara<sub>2</sub>-Hyp). HypBA2 directly liberates &beta;-Ara<sub>2</sub> from hydroxyproline-rich glycoproteins (HRGPs) such as carrot extensin and potato lectin. The family members are only found from prokaryote genomes, such as bacteria and actinomycetes.
  
 
== Kinetics and Mechanism ==
 
== Kinetics and Mechanism ==
HypBA2 is a retaining enzyme.The stereochemical course of the reaction was shown by transglycosylation activity toward 1-alkanols, and following <sup>1</sup>H-NMR and <sup>13</sup>C-NMR analysis of the methanol-adducted transglycosylation product.
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HypBA2 is a [[retaining]] enzyme. The stereochemical course of the reaction was shown by transglycosylation activity toward 1-alkanols, such as methanol; the resulting Ara''f''&beta;1-2Ara''f''&beta;-Me was identified by <sup>1</sup>H-NMR and <sup>13</sup>C-NMR analysis <cite>Fujita2011A</cite>.
  
 
== Catalytic Residues ==
 
== Catalytic Residues ==
Not known.
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The catalytic residues are not known but three conserved residues (Glu373, Asp515, and Glu713 in ''B. longum'' HypBA2) are the candidates based on mutagenesis and structural comparison <cite>Saito2020</cite>.
  
 
== Three-dimensional structures ==
 
== Three-dimensional structures ==
Not known.
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[[File:GH121-HypBA2.png|thumb|300px|right|'''Figure 1:''' &beta;-L-arabinobiosidase HypBA2 from ''Bifidobacterium longum''. The catalytic (&alpha;/&alpha;)<sub>6</sub> barrel domain is in green.]]
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The first solved 3-D structure was &beta;-L-arabinobiosidase HypBA2 from ''Bifidobacterium longum'' (PDB [{{PDBlink}}6m5a 6M5A]) <cite>Saito2020</cite>. The catalytic domain adops an (&alpha;/&alpha;)<sub>6</sub> barrel fold similar to [[GH142]], [[GH63]], [[GH78]], [[GH94]], and [[GH37]] ('''Figure 1''').
  
 
== Family Firsts ==
 
== Family Firsts ==
;First stereochemistry determination:  This was determined with HypBA2 enzyme by measurement of glycosyl transfer reactions to methanol and the <sup>1</sup>H-NMR and <sup>13</sup>C-NMR spectra.
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;First stereochemistry determination:  Shown to be [[retaining]] for HypBA2 by product analysis of glycosyl transfer reactions to methanol <cite>Fujita2011A</cite>.
;First catalytic nucleophile identification: No experimental proof.
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;First catalytic nucleophile identification: Predicted based on structural homology <cite>Saito2020</cite>, but currently no experimental proof.
;First general acid/base residue identification: No experimental proof.
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;First general acid/base residue identification: Predicted based on structural homology <cite>Saito2020</cite>, but currently no experimental proof.
;First 3-D structure: Not known.
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;First 3-D structure: &beta;-L-arabinobiosidase HypBA2 from ''Bifidobacterium longum'' <cite>Saito2020</cite>.
  
 
== References ==
 
== References ==
 
<biblio>
 
<biblio>
 
#Fujita2011A pmid=21149454
 
#Fujita2011A pmid=21149454
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#Fujita2011B pmid=21914802
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#Saito2020 pmid=32479540
 
</biblio>
 
</biblio>
  
 
[[Category:Glycoside Hydrolase Families|GH121]]
 
[[Category:Glycoside Hydrolase Families|GH121]]

Latest revision as of 14:18, 18 December 2021

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Glycoside Hydrolase Family GH121
Clan none
Mechanism retaining
Active site residues not known
CAZy DB link
http://www.cazy.org/GH121.html

Substrate specificities

This family of glycoside hydrolases contains β-L-arabinobiosidases, as demonstrated for HypBA2 from Bifidobacterium longum JCM 1217 [1]. HypBA2 liberates the disaccharide Arafβ1-2Araf (β-Ara2, a substrate of the GH127 β-L-arabinofuranosidase from B. longum JCM 1217 [2]) from unmodified Arafβ1-2Arafβ1-2Arafβ-hydroxyproline (Ara3-Hyp), but not Arafα1-3Arafβ1-2Arafβ1-2Arafβ-Hyp (Ara4-Hyp) or Arafβ1-2Arafβ-Hyp (Ara2-Hyp). HypBA2 directly liberates β-Ara2 from hydroxyproline-rich glycoproteins (HRGPs) such as carrot extensin and potato lectin. The family members are only found from prokaryote genomes, such as bacteria and actinomycetes.

Kinetics and Mechanism

HypBA2 is a retaining enzyme. The stereochemical course of the reaction was shown by transglycosylation activity toward 1-alkanols, such as methanol; the resulting Arafβ1-2Arafβ-Me was identified by 1H-NMR and 13C-NMR analysis [1].

Catalytic Residues

The catalytic residues are not known but three conserved residues (Glu373, Asp515, and Glu713 in B. longum HypBA2) are the candidates based on mutagenesis and structural comparison [3].

Three-dimensional structures

Figure 1: β-L-arabinobiosidase HypBA2 from Bifidobacterium longum. The catalytic (α/α)6 barrel domain is in green.

The first solved 3-D structure was β-L-arabinobiosidase HypBA2 from Bifidobacterium longum (PDB 6M5A) [3]. The catalytic domain adops an (α/α)6 barrel fold similar to GH142, GH63, GH78, GH94, and GH37 (Figure 1).

Family Firsts

First stereochemistry determination
Shown to be retaining for HypBA2 by product analysis of glycosyl transfer reactions to methanol [1].
First catalytic nucleophile identification
Predicted based on structural homology [3], but currently no experimental proof.
First general acid/base residue identification
Predicted based on structural homology [3], but currently no experimental proof.
First 3-D structure
β-L-arabinobiosidase HypBA2 from Bifidobacterium longum [3].

References

  1. Fujita K, Sakamoto S, Ono Y, Wakao M, Suda Y, Kitahara K, and Suganuma T. (2011). Molecular cloning and characterization of a beta-L-Arabinobiosidase in Bifidobacterium longum that belongs to a novel glycoside hydrolase family. J Biol Chem. 2011;286(7):5143-50. DOI:10.1074/jbc.M110.190512 | PubMed ID:21149454 [Fujita2011A]
  2. Fujita K, Takashi Y, Obuchi E, Kitahara K, and Suganuma T. (2011). Characterization of a novel β-L-Arabinofuranosidase in Bifidobacterium longum: functional elucidation of A DUF1680 family member. J Biol Chem. 2011;286(44):38079-38085. DOI:10.1074/jbc.M111.248690 | PubMed ID:21914802 [Fujita2011B]
  3. Saito K, Viborg AH, Sakamoto S, Arakawa T, Yamada C, Fujita K, and Fushinobu S. (2020). Crystal structure of β-L-arabinobiosidase belonging to glycoside hydrolase family 121. PLoS One. 2020;15(6):e0231513. DOI:10.1371/journal.pone.0231513 | PubMed ID:32479540 [Saito2020]

All Medline abstracts: PubMed