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Difference between revisions of "Carbohydrate Binding Module Family 8"
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== Functionalities == | == Functionalities == | ||
− | CelA and CelB are predicted to be important for amoebae release from spores as transcriptomic experiments show that their mRNA levels are low in dormant spores, they rise during germination and then rapidly disappear after germination <cite>Ramalingam1992</cite>. | + | CelA and CelB are predicted to be important for amoebae release from spores as transcriptomic experiments show that their mRNA levels are low in dormant spores, they rise during germination and then rapidly disappear after germination <cite>Ramalingam1992</cite>. Cellulase activities from differently sized cellulases is also shown to increase during spore germination <cite>Blume1991</cite> |
Revision as of 01:27, 30 April 2024
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CAZy DB link | |
http://www.cazy.org/CBM08.html |
Ligand specificities
DdCBM8, from the slime mold Dictyostelium discoideum CelA enzyme (270-6) binds insoluble forms of cellulose and glucomannan, there is no evidence for binding to oligosaccharides [1]. It appears to be broadly specific with both type A and type B CBM characteristics. The CelB protein (270-11) does not have cellulase activity but does show cellulose binding [2].
Structural Features
DdCBM8 is found C-terminal to the GH9 endo-(1,4)-beta-D-glucanase (cellulase) catalytic module of the CelA enzyme (270–6) and connected via a Thr-Glu-Thr-Pro type repeat linker [2].
Content in this section should include, in paragraph form, a description of:
- Fold: Structural fold (beta trefoil, beta sandwich, etc.)
- Type: Include here Type A, B, or C and properties
- Features of ligand binding: Describe CBM binding pocket location (Side or apex) important residues for binding (W, Y, F, subsites), interact with reducing end, non-reducing end, planar surface or within polysaccharide chains. Include examples pdb codes. Metal ion dependent. Etc.
Functionalities
CelA and CelB are predicted to be important for amoebae release from spores as transcriptomic experiments show that their mRNA levels are low in dormant spores, they rise during germination and then rapidly disappear after germination [2]. Cellulase activities from differently sized cellulases is also shown to increase during spore germination [3]
DdCBM8 from the endo-β-1,4-glucanase (CelA) of Dictyostelium discoideum
Content in this section should include, in paragraph form, a description of:
- Functional role of CBM: Describe common functional roles such as targeting, disruptive, anchoring, proximity/position on substrate.
- Most Common Associated Modules: 1. Glycoside Hydrolase Activity; 2. Additional Associated Modules (other CBM, FNIII, cohesin, dockerins, expansins, etc.)
- Novel Applications: Include here if CBM has been used to modify another enzyme, or if a CBM was used to label plant/mammalian tissues? Etc.
Family Firsts
- First Identified
- Insert archetype here, possibly including very brief synopsis.
- First Structural Characterization
- The first crystal structures are from DdCBM8, from the slime mold Dictyostelium discoideum [1], see 7T7Y and 7T7Z.
References
- Liberato MV, Campos BM, Tomazetto G, Crouch LI, Garcia W, Zeri ACM, Bolam DN, and Squina FM. (2022). Unique properties of a Dictyostelium discoideum carbohydrate-binding module expand our understanding of CBM-ligand interactions. J Biol Chem. 2022;298(5):101891. DOI:10.1016/j.jbc.2022.101891 |
- Ramalingam R, Blume JE, and Ennis HL. (1992). The Dictyostelium discoideum spore germination-specific cellulase is organized into functional domains. J Bacteriol. 1992;174(23):7834-7. DOI:10.1128/jb.174.23.7834-7837.1992 |
- Blume JE and Ennis HL. (1991). A Dictyostelium discoideum cellulase is a member of a spore germination-specific gene family. J Biol Chem. 1991;266(23):15432-7. | Google Books | Open Library