User:Shinya Fushinobu

I am an Associate Professor at Laboratory of Enzymology in Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo located in Tokyo, Japan. Raised in Kure, Hiroshima, Japan. I obtained Ph.D degree in Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo in 1999. My research interests concern structure and function of enzymes, mainly those of Carbohydrate-Active enZymes. My home page is here. I contributed to three-dimensional structure determination of

• GH1 Phanerochaete chrysosporium β-glucosidase 1B (BGL1B) [1]
• GH3 Kluyveromyces marxianus β-glucosidase (KmBglI) [2]
• GH8 Bacillus halodurans reducing-end xylose-releasing exo-oligoxylanase (Rex) [3]
• GH10 Clostridium stercorarium xylanase B (XynB) [4]
• GH11 Aspergillus kawachii xylanase C (XynC) [5]
• GH42 Thermus thermophilus β-galactosidase (A4-β-Gal) Family First [6]
• GH54 Aspergillus kawachii α-L-arabinofuranosidase B (AkAbfB) Family First plus identification of CBM42 [7]
• GH55 Phanerochaete chrysosporium β-1,3-glucanase (Lam55A) Family First [8]
• GH57 Thermococcus litoralis 4-α-glucanotransferase (TLGT) Family First [9]
• GH94 Vibrio proteolyticus chitobiose phosphorylase (ChBP) Family First [10]
• GH94 Cellvibrio gilvus cellobiose phosphorylase (CBP) [11]
• GH101 Bifidobacterium longum endo-α-N-acetylgalactosaminidase (EngBF) [12]
• GH112 Bifidobacterium longum galacto-N-biose/lacto-N-biose I phosphorylase (GLNBP) Family First [13]
• PL20 Trichoderma reesei endo-β-1,4-glucuronan lyase (TrGL) Family First [14]
• CBM28 in Clostridium josui Cel5A (CjCBM28) [15]

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2. Yoshida E, Hidaka M, Fushinobu S, Koyanagi T, Minami H, Tamaki H, Kitaoka M, Katayama T, Kumagai H. Role of a PA14 domain in determining substrate specificity of a glycoside hydrolase family 3 β-glucosidase from Kluyveromyces marxianus. Biochem J. 2010 Oct 1;431(1):39-49.

   Note: Due to a problem with PubMed data, this reference is not automatically formatted. Please see these links out: DOI:10.1042/BJ20100351 PMID: 20662765

7. Miyanaga A, Koseki T, Matsuzawa H, Wakagi T, Shoun H, and Fushinobu S. (2004). Crystal structure of a family 54 alpha-L-arabinofuranosidase reveals a novel carbohydrate-binding module that can bind arabinose. J Biol Chem. 2004;279(43):44907-14. DOI:10.1074/jbc.M405390200 |
8. Ishida T, Fushinobu S, Kawai R, Kitaoka M, Igarashi K, and Samejima M. (2009). Crystal structure of glycoside hydrolase family 55 {beta}-1,3-glucanase from the basidiomycete Phanerochaete chrysosporium. J Biol Chem. 2009;284(15):10100-9. DOI:10.1074/jbc.M808122200 |
9. Imamura H, Fushinobu S, Yamamoto M, Kumasaka T, Jeon BS, Wakagi T, and Matsuzawa H. (2003). Crystal structures of 4-alpha-glucanotransferase from Thermococcus litoralis and its complex with an inhibitor. J Biol Chem. 2003;278(21):19378-86. DOI:10.1074/jbc.M213134200 |
10. Hidaka M, Honda Y, Kitaoka M, Nirasawa S, Hayashi K, Wakagi T, Shoun H, and Fushinobu S. (2004). Chitobiose phosphorylase from Vibrio proteolyticus, a member of glycosyl transferase family 36, has a clan GH-L-like (alpha/alpha)(6) barrel fold. Structure. 2004;12(6):937-47. DOI:10.1016/j.str.2004.03.027 |