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Glycoside Hydrolase Family 45

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This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.


Glycoside Hydrolase Family GH45
Clan none
Mechanism inverting
Active site residues known (but see discussion)
CAZy DB link
http://www.cazy.org/fam/GH45.html


Substrate specificities

Endoglucanases (EC 3.2.1.4). Mainly the hydrolysis of soluble beta 1,4 glucans.


Kinetics and Mechanism

The enzymes act with inversion of anomeric configuration to generate the alpha-D glucoside as product. Based upon the structure of the Humicola insolens endoglucanase V (now known as Cel45)[1][2] it was concluded that Asp121 acted as the catalytic acid (implied by its hydrogen bonding to the glycosidic oxygen) and that the most likely catalytic base would be Asp10, appropriately positioned "below" the sugar plane. As with many inverting enzymes the base assignment is less secure than that of the acid.


Catalytic Residues

Content is to be added here.


Three-dimensional structures

Content is to be added here.


Family Firsts

First sterochemistry determination
Cite some reference here, with a short (1-2 senetence) explanation [3].
First catalytic nucleophile identification
Cite some reference here, with a short (1-2 senetence) explanation [4].
First general acid/base residue identification
Cite some reference here, with a short (1-2 senetence) explanation [5].
First 3-D structure
The Humicola insolens EGV (now Cel45) by the Davies group [1].

References

  1. Davies GJ, Dodson GG, Hubbard RE, Tolley SP, Dauter Z, Wilson KS, Hjort C, Mikkelsen JM, Rasmussen G, and Schülein M. (1993). Structure and function of endoglucanase V. Nature. 1993;365(6444):362-4. DOI:10.1038/365362a0 | PubMed ID:8377830 [Davies1993]
  2. Davies GJ, Tolley SP, Henrissat B, Hjort C, and Schülein M. (1995). Structures of oligosaccharide-bound forms of the endoglucanase V from Humicola insolens at 1.9 A resolution. Biochemistry. 1995;34(49):16210-20. DOI:10.1021/bi00049a037 | PubMed ID:8519779 [Davies1995]
  3. Sinnott, M.L. (1990) Catalytic mechanisms of enzymic glycosyl transfer. Chem. Rev. 90, 1171-1202. DOI: 10.1021/cr00105a006

    [4]
  4. Robert V. Stick and Spencer J. Williams. (2009) Carbohydrates. Elsevier Science. [3]

All Medline abstracts: PubMed

[[Category:Glycoside Hydrolase Families|GHnnn]]